Assaying binding capacity of Cu,ZnSOD and MnSOD: Demonstration of their localization in cells and tissues

Emerit, I; Filipe, Paulo; Freitas, João; Fernandes, A. D. Lemos; Garban, Frédéric; Vassy, Jany

doi:10.1016/s0076-6879(02)49347-0

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…Also in agreement with this view is the protective effect of SOD, which is optimal when the enzyme is added 30 min before addition of CF or during the first 24 h. The latter is analogous to the time course of inhibition of CF formation by SOD. Our experiments with fluorescently labelled SOD indicated that the enzyme protects the cells not only by dismutating extracellular superoxide, but that it binds to the cell membrane in particular of monocytes/macrophages (Emerit et al 1996 and 2002). The superoxide generation by these cells diminishes, and the formation of CF is reduced.…”

Section: Mecanisms Of Cf Formation and Cf Actionmentioning

confidence: 80%

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Emerit

2007

Biomark�Insights

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The formation of clastogenic factors (CF) and their damaging effects are mediated by superoxide, since superoxide dismutase is regularly protective. CF are produced via superoxide and stimulate the production of superoxide by monocytes and neutrophils. This results in a selfsustaining and longlasting process of clastogenesis, which may exceed the DNA repair system and ultimately lead to cancer (Emerit, 1994). An increased cancer risk is indeed observed in conditions accompanied by CF formation. These include irradiated persons, patients with chronic inflammatory diseases, HIV-infected persons and the chromosomal breakage syndromes ataxia telangiectasia, Bloom’s syndrome and Fanconi’s anemia. Biochemical analysis has identified lipid peroxidation products, arachidonic acid metabolites, nucleotides of inosine and cytokines, in particular tumor necrosis factor alpha, as the clastogenic and also superoxide stimulating components of CF. Due to their chromosome damaging effects, these oxidants can be detected with classical cytogenetic techniques. Their synergistic action renders the CF-test particularly sensitive for the detection of a pro-oxidant state. Correlations were observed between CF and other biomarkers of oxidative stress such as decreases in total plasma thiols or increases in TBARS or chemiluminescence. Correlations between CF and disease activity, between CF and radiation exposure, suggest the study of CF for monitoring these conditions. CF may also be useful as biochemical markers and intermediate endpoints for the evaluation of promising antioxidant drugs.CF formation represents a link between chronic inflammation and carcinogenesis. Prophylactic use of superoxide scavengers as anticarcinogens is therefore suggested.

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Section: Mecanisms Of Cf Formation and Cf Actionmentioning

confidence: 80%

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Emerit

2007

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“…Extracellular matrix and the cell surface is the main EC SOD (SOD3) localization (28, 29) and structural differences between SOD1 and SOD3 seem to be the main factor that influences their activity and the place of action. Emerit et al, (30) in their experiments with fluorescently labeled SOD clearly showed that the enzyme binds to cellular membranes and the intensity of binding varies according to cell type. The differences observed for binding were concomitant with decreased inhibition of stimulated superoxide production.…”

Section: Discussionmentioning

confidence: 99%

Activity of CuZn‐superoxide dismutase, catalase and glutathione peroxidase in erythrocytes in kidney allografts during reperfusion in patients with and without delayed graft function

Domański¹,

Dołęgowska²,

Safranow³

et al. 2005

Clinical Transplantation

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“…Extracellular matrix and the cell surface is the main EC SOD (SOD3) localization (Marklund, , 1994) and structural differences between SOD1 and SOD3 seem to be the main factor that influences their activity and the place of action. In experiments with fluorescently labeled SOD (Emerit et al, 2002) clearly, it was showed that the enzyme binds to cellular membranes and the intensity of binding varies according to cell type. The differences observed for binding were concomitant with decreased inhibition of stimulated superoxide production.…”

Section: Activity Of Cuzn-superoxide Dismutase Catalase and Glutathimentioning

confidence: 99%

Ischemia-Reperfusion Injury in the Transplanted Kidney Based on Purine Metabolism Markers and Activity of the Antioxidant System

Domański¹,

Kłoda²,

Ciechanowski³

2012

Organ Donation and Transplantation - Public Policy and Clinical Perspectives

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Assaying binding capacity of Cu,ZnSOD and MnSOD: Demonstration of their localization in cells and tissues

Cited by 6 publications

References 5 publications

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Activity of CuZn‐superoxide dismutase, catalase and glutathione peroxidase in erythrocytes in kidney allografts during reperfusion in patients with and without delayed graft function

Ischemia-Reperfusion Injury in the Transplanted Kidney Based on Purine Metabolism Markers and Activity of the Antioxidant System

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