Activity of CuZn‐superoxide dismutase, catalase and glutathione peroxidase in erythrocytes in kidney allografts during reperfusion in patients with and without delayed graft function

Domański, Leszek; Dołęgowska, Barbara; Safranow, Krzysztof; Różański, Jacek; Myślak, Marek; Romanowski, M; Sieńko, Jerzy; Sulikowski, Tadeusz; Ostrowski, Marek; Kędzierska, Karolina; Domański, Maciej; Chlubek, Dariusz; Pawlik, Andrzej; Ciechanowski, Kazimierz

doi:10.1111/j.1399-0012.2005.00442.x

Cited by 15 publications

(7 citation statements)

References 27 publications

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“…Levels of antioxidant enzymes have been reported to increase (23,24), decrease (12,16), or remain unchanged (25,26) after KTx. A wide range of protective substances, such as antioxidant enzymes, might potentially elicit a protective effect by limiting the production of ROS and the damage of oxidative stress after I/R injury of a kidney graft.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress in Kidney Transplantation

Fonseca¹,

Reguengo²,

Almeida³

et al. 2014

Transplantation

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress in Kidney Transplantation

Fonseca¹,

Reguengo²,

Almeida³

et al. 2014

Transplantation

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“…Blood samples were collected according to a previously described protocol (16). To determine prereperfusion levels/ activity of analyzed parameters, a blood sample was taken from the iliac vein before anastomosing kidney vessels with the recipient's iliac vessels (time 0).…”

Section: Methodsmentioning

confidence: 99%

Clinical Analysis of Perioperative Complement Activity during Ischemia/Reperfusion Injury following Renal Transplantation

Błogowski

Dołęgowska²,

Sałata³

et al. 2012

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives The complement cascade seems to be an important mediator modulating renal ischemia/reperfusion injury. This study analyzed whether significant changes occur in the levels of a terminal panel of complement molecules (C3a, C5a, and C5b-9/membrane attack complex) during the early phase of human kidney allograft reperfusion and evaluated the potential association of these changes with clinical post-transplant graft function in kidney transplant recipients.Design, setting, participants, & measurements Seventy-five renal transplant recipients undergoing transplantation between 2004 and 2006 were enrolled in the study and divided into early, slow, and delayed graft function groups. Blood samples were collected perioperatively during consecutive minutes of allograft reperfusion from the renal vein. Levels of complement molecules were measured using ELISA.Results Analysis revealed no significant changes in C3a and C5a levels throughout reperfusion. The main complement molecule that was significantly associated with post-transplant graft function was C5b-9/membrane attack complex; throughout the reperfusion period, perioperative levels of C5b-9/membrane attack complex were around two to three times higher in delayed graft function patients than early and slow graft function individuals (P,0.005). In addition, C5b-9/membrane attack complex levels had a relatively high clinical sensitivity and specificity (70%-87.5%) for the prediction of early and long-term (1 year) posttransplant allograft function.Conclusions This clinical study supports a role for the complement cascade in delayed graft function development. However, additional studies are needed to elucidate the exact mechanisms responsible for this phenomenon. In addition, perioperative measurements of C5b-9/membrane attack complex are highlighted as promising potential clinical markers of post-transplant renal allograft function.

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“…17 In our previous study we showed that catalase activity during kidney graft reperfusion correlates with hypoxanthine concentration in the graft's renal vein during reperfusion. 18 During ischaemia, elevated concentrations of hypoxanthine may occur due to the breakdown of adenosine triphosphate (ATP). When xanthine oxidase converts hypoxanthine to xanthine in the presence of molecular oxygen, superoxide radical (O2 -) is generated.…”

Section: Discussionmentioning

confidence: 99%

The association of –262C/T polymorphism in the catalase gene and delayed graft function of kidney allografts

Dutkiewicz¹,

Domański²,

Bińczak-Kuleta³

et al. 2010

Nephrology

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Activity of CuZn‐superoxide dismutase, catalase and glutathione peroxidase in erythrocytes in kidney allografts during reperfusion in patients with and without delayed graft function

Abstract: Erythrocyte-antioxidative system in graft's vein remain stable during the early phase of reperfusion. The results of the study suggest that further studies on extracellular enzymes are required for the assessment of antioxidant system in the conditions of ischemia/reperfusion.

Cited by 15 publications

References 27 publications

Oxidative Stress in Kidney Transplantation

Oxidative Stress in Kidney Transplantation

Clinical Analysis of Perioperative Complement Activity during Ischemia/Reperfusion Injury following Renal Transplantation

The association of –262C/T polymorphism in the catalase gene and delayed graft function of kidney allografts

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