Assay of UDP-galactose 4-epimerase.

Shin, Young Sook; Rücker, Axel von; Rieth, M.; Endres, W.

doi:10.1093/clinchem/28.11.2332

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Cited by 7 publications

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“…For each stage at least eight animals were killed. Endogenous GALE activity was measured and calculated as described by Shin et al [12, 13].…”

Section: Methodsmentioning

confidence: 99%

Differential UDP‐galactose‐4′‐epimerase (GALE) enzymatic activity and mRNA expression in the rat mammary gland during lactation

Prestoz¹,

Daude²,

Cournu³

et al. 1998

FEBS Letters

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We have investigated the UDP-galactose-4P-epimerase (GALE) enzymatic activity and mRNA expression in the rat mammary gland during lactation. We report a dramatic increase in the GALE enzymatic activity correlated with an increase in the mRNA transcript expression. These results indicate a transcriptional regulation of the enzyme during lactation in the rat mammary gland. Our data are of double interest for further investigation: first, the mammary gland provides a suitable model for the characterisation of the transcriptional regulation elements of GALE which are still unknown in mammals; second, GALE expression could help to compensate UDP-galactose deficiency in classic galactosaemia.z 1998 Federation of European Biochemical Societies.

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“…For each stage at least eight animals were killed. Endogenous GALE activity was measured and calculated as described by Shin et al [12, 13].…”

Section: Methodsmentioning

confidence: 99%