Assay of synovial fluid hyaluronic acid using high-performance liquid chromatography of hyaluronidase digests

Payan, E.; Jouzeau, Jean‐Yves; Lapicque, François; Müller, Nora; Payan, Jean-Paul; Gegout, P.; Bertin, Philippe; Netter, Patrick

doi:10.1016/0378-4347(91)80106-m

Cited by 19 publications

(13 citation statements)

References 21 publications

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“…The inter-subject variability is large: this may partly be explained by the marked influence of physical activity on HA concentrations (Chichibu et al 1989;Engstrom-Laurent 1989;Payan et al 1991). The inter-subject variability is large: this may partly be explained by the marked influence of physical activity on HA concentrations (Chichibu et al 1989;Engstrom-Laurent 1989;Payan et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Sodium naproxen: concentration and effect on inflammatory response mediators in human rheumatoid synovial fluid

Bertin

Lapicque

Payan

et al. 1994

Eur J Clin Pharmacol

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Twelve patients suffering from rheumatoid arthritis and having swollen knees were treated with 1.1 g/day of sodium naproxen administered in one dose, daily for 5 days. The 72-h wash-out period was verified by the absence of any nonsteroidal anti-inflammatory drug using a HPLC screening. Blood and synovial fluid samples were drawn just before treatment and 24 h after the last dose. Eicosanoids (PGE2, 6-keto-PGF1 alpha, TXB2, LTB4, LTC4) in synovial fluid were determined by immunoenzymatic assays. In plasma and synovial fluid, hyaluronic acid was assayed by radiometric assay and sodium naproxen by HPLC. Free drug was determined by equilibrium dialysis. Statistical analysis used nonparametric tests. Pain relief (evaluated on a visual scale), morning stiffness, and scores on the Lee and Ritchie indices all decreased significantly, as did PGE2 and LTB4 concentrations. The decrease in 6-keto-PGF1 alpha and TXB2 was not significant. No significant change was found for LTC4 and hyaluronic acid. Total concentrations of sodium naproxen were equivalent in plasma (16.1 micrograms.ml-1) and synovial fluid (18.9 micrograms.ml-1). Free fractions were significantly higher in synovial fluid (0.14%) than in plasma (0.11%), as shown by binding of the drug to human serum albumin, at various protein concentrations. Interestingly, the clinical efficacy, as shown by decreases in morning stiffness and in the Lee index score, correlated with the free concentration of naproxen in synovial fluid.

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Section: Discussionmentioning

confidence: 99%

Sodium naproxen: concentration and effect on inflammatory response mediators in human rheumatoid synovial fluid

Bertin

Lapicque

Payan

et al. 1994

Eur J Clin Pharmacol

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“…For higher formation times, HA contents were determined by HPLC after enzymatic digestion of the calcium media (Payan et al, 1991). Hyaluronate was therefore assayed from the calcium chloride solution.…”

Section: Ha Contentmentioning

confidence: 99%

Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism

Oerther,

Le Gall,

Payan

et al. 1999

Biotechnol. Bioeng.

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With the aim of producing a biomaterial for surgical applications, the alginate-hyaluronate association has been investigated to combine the gel-forming properties of alginate with the healing properties of hyaluronate. Gels were prepared by diffusion of calcium into alginate-hyaluronate mixtures, with an alginate content of 20 mg/mL. The hyaluronate source was shown to have significant effect on the aspect and the properties of the gels. The gels have viscoelastic behaviour and the transient measurements carried out in creep mode could be interpreted through a Kelvin-Voigt generalised model: experimental data led to the steady state hardness and a characteristic viscosity of the gel. Gels prepared from Na rooster comb hyaluronate with weight ratio up to 0.50 have satisfactory mechanical properties, and fully stable gels are obtained after a few days; on the contrary, use of lower molecular weight hyaluronate led to loose gels for hyaluronate contents over 0.25. Gel formation was investigated by measurements of the exchange fluxes between the calcium chloride solution and the forming gel, which allowed thorough investigations of the occuring diffusion phenomena of water, calcium ion and hyaluronate. Strong interactions of water with hyaluronate reduce significantly the rate of weight loss from the gel beads and allows higher water content in steady-state gels. Calcium content in the gel samples could be correlated to the actual alginate concentration, whatever the nature and the weight ratio of hyaluronate.

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“…is equivalent to 1 turbidity reducing unit). Enzymatic degradation was performed according to the method described by Payan et al15…”

Section: Methodsmentioning

confidence: 99%

“…It is composed of alternating units of glucuronic acid and N ‐acetyglucosamine that is linked by β (1→3) and β (1→4) glycosidic bonds 9–11. In the human body, natural HA is degraded rapidly by enzymes (hyaluronidases) resulting in a short half life ( t 1/2 = 0.5 day) in skin and joints, suggesting that one third of the endogenous HA is turned over each day 15, 16–19…”

Section: Introductionmentioning

confidence: 99%

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Comparative stability of the bioresorbable ferric crosslinked hyaluronic acid adhesion prevention solutions

2013

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The Intergel® ferric crosslinked hyaluronate (FeHA) adhesion prevention solution (APS) (FDA) is associated with serious post-operative complications (Henley, http://www.lawyersandsettlements.com/features/gynecare-intergel/intergel-timeline.html, 2007; FDA, 2003; Roman et al., Fertil Steril 2005, 83 Suppl 1:1113-1118; Tang et al., Ann Surg 2006;243(4):449-455; Wiseman, Fertil Steril 2006;86(3):771; Wiseman, Fertil Steril 2006;85(4):e7). This prompted us to examine the in situ stability of crosslinked HA materials to hyaluronidase lyase degradation. Variables such as ferric ionic crosslink density, HA concentration, gel geometry, and molecular weight (MW) of HA polymer were studied. Various formulations of the crosslinked "in house" [Isayeva et al., J Biomed Mater Res: Part B - Appl Biomater 2010, 95B (1):9-18] FeHA (0.5%, w/v; 30, 50, 90% crosslinked), the Intergel® FeHA (0.5%, w/v; 90%), and the non-crosslinked HA (0.05-0.5%, w/v) were degraded at a fixed activity of hyaluronidase lyase from Streptomyces hyalurolyticus (Hyase) at 37°C over time according to the method [Payan et al., J Chrom B: Biomed Sci Appl 1991;566(1):9-18]. Under our conditions, the data show that the crosslink density affects degradation the most, followed by HA concentration and then gel geometry. We found that MW has no effect. Our results are one possible explanation of the observations that the Intergel® FeHA APS (0.5%, w/v; 90%) material persisted an order of magnitude longer than expected [t1/2 = 500 hrs vs. t1/2 = 50 hrs (FDA; Johns et al., Fertil Steril 1997;68(1):37-42)]. These data also demonstrate the sensitivity of the in vitro hyaluronidase assay to predict the in situ stability of crosslinked HA medical products as previously reported [Sall et al., Polym Degrad Stabil 2007;92(5):915-919].

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Assay of synovial fluid hyaluronic acid using high-performance liquid chromatography of hyaluronidase digests

Cited by 19 publications

References 21 publications

Sodium naproxen: concentration and effect on inflammatory response mediators in human rheumatoid synovial fluid

Sodium naproxen: concentration and effect on inflammatory response mediators in human rheumatoid synovial fluid

Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism

Comparative stability of the bioresorbable ferric crosslinked hyaluronic acid adhesion prevention solutions

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