ASSAY OF POLY-β-HYDROXYBUTYRIC ACID

Law, John H.; Slepecky, Ralph A.

doi:10.1128/jb.82.1.33-36.1961

Cited by 729 publications

(268 citation statements)

References 13 publications

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“…PHB was obtained from freeze-dried cells using a hypochlorite treatment followed by extraction in hot chloroform (Williamson and Wilkinson, 1958). The concentration of PHB was measured according to the assay of Law and Slepecky (1961).…”

Section: Morphological Observationsmentioning

confidence: 99%

Microaerobic and anaerobic metabolism of a Methylocystis parvus strain isolated from a denitrifying bioreactor

Vecherskaya

Dijkema

Ramírez-Saad

et al. 2009

Environ Microbiol Rep

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An obligate methanotrophic bacterium, strain MTS, was isolated from a methane-fed microaerobic denitrifying bioreactor. 16S rRNA and DNA-DNA hybridization analysis revealed that this organism was most closely related to Methylocystis parvus, a Type II methanotroph, belonging to the α-subclass of the Proteobacteria. The metabolism of the bacterium under microaerobic and anaerobic conditions was studied by (13) C-NMR. (13) C-labelled poly-β-hydroxybutyrate (PHB) formation occurred in cell suspensions incubated with (13) C-labelled methane at low (5-10%) oxygen concentration. Under these conditions low levels of succinate, acetate and 2,3-butanediol were formed and excreted into the culture medium. Intracellular PHB degradation was observed in intact cells under anaerobic conditions in the absence of an exogenous carbon source during a long-term incubation of 90 days. Multiple (13) C-labelled β-hydroxybutyrate, butyrate, acetate, acetone, isopropanol, 2,3-butanediol and succinate were identified as products in in vivo(13) C-NMR spectra and in the spectra of culture medium during the dynamic PHB degradation. The isolated obligate methanotroph clearly shows a fermentative metabolism of PHB under anaerobic conditions. The excreted products may serve as substrates for denitrifying bacteria.

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Section: Morphological Observationsmentioning

confidence: 99%

Microaerobic and anaerobic metabolism of a Methylocystis parvus strain isolated from a denitrifying bioreactor

Vecherskaya

Dijkema

Ramírez-Saad

et al. 2009

Environ Microbiol Rep

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“…This makes it impossible to design a general protocol for the isolation of cPHB. In bacteria, PHB is usually isolated by the method of Law and Slepecky [8] which is based on the relative stability of the polymer to alkaline hypochlorite, a reagent that degrades most other cellular macromolecules, and the insolubility of the polymer in water, alcohol, and acetone. The behavior of cPHB in this procedure cannot be predicted.…”

Section: Isolation Of Cphbmentioning

confidence: 99%

“…cPHB has been identified by chemical assay [6], and in some cases by immunoassay [7] and I H NMR. For quantitative chemical determinations, the dried sample may be hydrolyzed in concentrated sulfuric acid to convert total PHB to crotonic acid [8]. The crotonic acid can then be isolated and quantified [6][7][8][9] with a sensitivity limit of 10 ng PHB.…”

Section: Analysis Of Cphbmentioning

confidence: 99%

“…For quantitative chemical determinations, the dried sample may be hydrolyzed in concentrated sulfuric acid to convert total PHB to crotonic acid [8]. The crotonic acid can then be isolated and quantified [6][7][8][9] with a sensitivity limit of 10 ng PHB. In using this procedure, it is necessary to rule out or evaluate the contribution of the monomer, /3-hydroxybutyrate.…”

Section: Analysis Of Cphbmentioning

confidence: 99%

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Biological complexes of poly-β-hydroxybutyrate

Reusch¹

1992

FEMS Microbiology Letters

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“…Varied acid treatment is usually done to hydrolyze the linkages in hemicellulose and cellulose chains in bagasse to produce xylose, glucose, and other sugars. 16 In this study, we extracted the remaining sugar component of bagasse by subjecting it to boiling water and fed it as a substrate to the bacteria for PHA accumulation under stress conditions. It may therefore be possible to use the lignocellulosic component of the bagasse fibers as a substrate for another production process, thereby molding each production process for a biobased economy.…”

Section: Utilizing Sugarcane Bagasse As a Substrate For Pha Productionmentioning

confidence: 99%

Production of polyhydroxyalkanoates (PHA) from a non‐lignocellulosic component of sugarcane bagasse: fueling a biobased economy

Tyagi

Saxena

Sharma

2018

Biofuels Bioprod Bioref

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The present study examines the use of residual sugar from sugarcane bagasse for the production of polyhydroxyalkanoates (PHA), a class of biodegradable microbial polymers. To achieve this, 26 bacterial isolates were screened for PHA production. From the strains isolated, ART_MKT2E was found to produce PHA and was selected to discover its capability to use a bagasse‐based medium (BGM). To date, the lignocellulosic component of bagasse has been used as a substrate by treating bagasse with acids. This study demonstrates the use of residual sugar from bagasse as a substrate for PHA production. The concentration of bagasse filtrate was found to be optimum at 60%, along with yeast extract and additional salts (pH 7.0 ± 0.5). The maximum PHA production (w/w) was 55%. These results indicate the successful use of non‐lignocellulosic BGM. The remaining lignocellulosic component of bagasse could be fed as a substrate for another production process, promoting a sustainable, biobased economy. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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ASSAY OF POLY-β-HYDROXYBUTYRIC ACID

Cited by 729 publications

References 13 publications

Microaerobic and anaerobic metabolism of a Methylocystis parvus strain isolated from a denitrifying bioreactor

Microaerobic and anaerobic metabolism of a Methylocystis parvus strain isolated from a denitrifying bioreactor

Biological complexes of poly-β-hydroxybutyrate

Production of polyhydroxyalkanoates (PHA) from a non‐lignocellulosic component of sugarcane bagasse: fueling a biobased economy

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