Assay of Malondialdehyde in Biological Fluids by Gas Chromatography-Mass Spectrometry

Alterations in the diabetic myocardial proteome coupled with increased myocardial oxidative stress underlies diabetic cardiomyopathy

“…malondialdehyde, lipid hydroperoxides) [23,33]. Furthermore, little is known about the relation between oxidative stress and changes at the cardiac proteome level.…”

Inducible Malondialdehyde Pools in Zones of Cell Proliferation and Developing Tissues in Arabidopsis

“…This toxicity of MDA, and its association with pathogenesis and lipid oxidation, has spawned a large literature. But the fact that MDA is damaging to cells is striking in light of the fact that the aldehyde is widespread in the healthy organs of both vertebrates (10,11) and plants (12,13).…”

“…2 H2-MDA (10,11). TBA is a nonspecific chemical reagent for MDA detection, but its specificity is enhanced by using fluorescence detection of MDA-TBA adducts (16,17).…”

“…Gas Chromatography/Mass Spectrometry (GC/MS)-Quantitation based on a specific pentafluorophenylhydrazine (PFPH)-MDA adduct was used with ( 2 H 2 )-MDA as an internal standard (IS) (10). Plant material (about 150 mg) was harvested, frozen in liquid nitrogen, and ground to a fine powder.…”

“…The frozen powder was added to PBS containing 1 mM deferoxamine mesylate, 50 M 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 50 pmol IS and 6.6 N H 2 SO 4 (10 l) and incubated for 10 min on ice, in the dark. Sodium tungstate (0.3 M, 75 l) was then added and the samples centrifuged to precipitate protein (10). The supernatant was combined with buffer (0.21 M citric acid, 0.58 M Na 2 HPO 4 ; pH 4) and 50 l of PFPH reagent (5 mg ml Ϫ1 in water) and incubated for 3 h at 22°C.…”