Assay of Cholinesterase in Clinical Chemistry

The Effect of Storage at Different Temperatures on Cholinesterase Activity in Human Serum

“…After the start of the prthotopic liver transplantation Programme at The University Hospital Groningen in 1979, the need arose for a rapidly and easily determined parameter for the synthesis function of the liver.-The enzyme cholinesterase (EC 3.1.1.7) appeared to be suitäble for this purpose (1), since it is probably synthesized exclusively in the liver (2). Apart from the liver, cholinesterase is also föund in plasma* serum, pancreas, kidney, heart, intestinal mucosa, skin and brain tissue.…”

“…Apart from the liver, cholinesterase is also föund in plasma* serum, pancreas, kidney, heart, intestinal mucosa, skin and brain tissue. Cholinesterase hydrolyses choline esters according to the following reaction: Besides the value of cholinesterase in testing liver synthesis function, liver cholinesterase is also known to be a sensitive parameter in cases of intoxication with organophosphorus compounds, e. g. parathion, malathion and carbamates (2,3). It is known that the activity of several enzymes is influenced by storage.…”

Comparison of Substrates for Measuring Serum Choline Esterase Activity in Hepato-Biliary Disease

“…The optimal pH for the assay varies with the type and ionic strength of the buffer, with the substrate concentration, and also with the enzyme variâ nts (2,25). The optimal pH for the assay varies with the type and ionic strength of the buffer, with the substrate concentration, and also with the enzyme variâ nts (2,25).…”

“…The optimal pH for the assay varies with the type and ionic strength of the buffer, with the substrate concentration, and also with the enzyme variâ nts (2,25). However, a pH of 7.5, äs used in the present work, is generally preferred (2), because there is an apparent increase in spontaneous hydrolysis of Substrate with increasing pH (2). However, a pH of 7.5, äs used in the present work, is generally preferred (2), because there is an apparent increase in spontaneous hydrolysis of Substrate with increasing pH (2).…”

Short Communication

“…Genetic variants detected by inhibition studies, such as dibucaine-resistant ("atypical") or fluoride-resistant cholinesterases are usually defined under the same reaction conditions (2)(3)(4)(5)(6)(7)(8)(9). Genetic variants detected by inhibition studies, such as dibucaine-resistant ("atypical") or fluoride-resistant cholinesterases are usually defined under the same reaction conditions (2)(3)(4)(5)(6)(7)(8)(9).…”