Assay of Chlordiazepoxide and Demoxepam in Chlordiazepoxide Formulations by Difference Spectrophotometry

Davidson, A.G.

doi:10.1002/jps.2600730114

Cited by 14 publications

(5 citation statements)

References 17 publications

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“…The 1,4-benzodiazepines comprise a class of drug in which several sites of protonation and deprotonation exist, and which exhibit marked changes of spectral properties as a result of variation of pH (Barrett et a1 1973). These spectral transformations have been utilized in the simultaneous difference spectrophotometric assay of chlordiazepoxide and its hydrolysis product, demoxepam (Davidson 1984b) and in the assay of diazepam, clonazepam and medazepam (Abdel-Hamid et a1 1984). As part of a continuing research programme, the present study was undertaken to develop a difference spectrophotometric assay for nitrazepam and to evaluate its suitability as s stability-indicating assay for the measurement of the reaction kinetics of the hydrolysis of nitrazepam in alkaline solution.…”

Section: Difference Spectrophotometric Assay Of Nitrazepam In Tablet mentioning

confidence: 99%

Difference spectrophotometric assay of nitrazepam in tablet formulations

Davidson

1989

Journal of Pharmacy and Pharmacology

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A difference spectrophotometric procedure is described for the assay of nitrazepam in tablet formulations. The method is based on the measurement of absorbance at 282 nm of a solution of the tablet extract in 0.1 M hydrochloric acid (pH 1) relative to that of an equimolar solution in 0.1 M sodium hydroxide (pH 13). The method is precise and selective for nitrazepam in the presence of the tablet excipients and 2-amino-5-nitrobenzophenone, the principal hydrolysis product of nitrazepam. The absence of a constant isosbestic point in the difference spectrum of nitrazepam during hydrolysis in alkaline solution indicates the presence of a previously unrecognized intermediate hydrolysis product.

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Section: Difference Spectrophotometric Assay Of Nitrazepam In Tablet mentioning

confidence: 99%

Difference spectrophotometric assay of nitrazepam in tablet formulations

Davidson

1989

Journal of Pharmacy and Pharmacology

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“…There are several assay methods for the determination of CLI (4) and CHLOR (5)(6)(7)(8)(9)(10)(11) individually in formulations, biological fluids, and in combination with other drugs. Also a spectrophoto-metric (1) and a simple high-performance liquid chromatography (HPLC) (12) method have been developed for the combination of the two drugs.…”

Section: Introductionmentioning

confidence: 99%

Stability-Indicating HPLC Method for Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms

Pathak

Rai

Rajput

2010

Journal of Chromatographic Science

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The study describes development and subsequent validation of a stability indicating reverse-phase high-performance liquid chromatography method for the simultaneous estimation of clidinium bromide (CLI) and chlordiazepoxide (CHLOR) from their combination drug product. Chromatographic separations are performed at ambient temperature on a Phenomenex Luna C(18) (250 mm x 4.6 mm, i.d., 5 microm) column using a mobile phase consisting of potassium dihydrogen phosphate buffer (0.05 M, pH 4.0 adjusted with 0.5% orthophosphoric acid)-methanol- acetonitrile (40:40:20, v/v/v). The flow rate is 1.0 mL/min, and the detection wavelength is 220 nm. The method is validated with respect to linearity, precision, accuracy, system suitability, and robustness. The utility of the procedure is verified by its application to marketed formulations that were subjected to accelerated degradation studies. The method distinctly separated the drug and degradation products even in actual samples. The products formed in marketed tablet dosage forms are similar to those formed during stress studies.

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“…Literature survey revealed determination of major impurity of CLR by an UV method[10] and HPLC method with FTIR and UV detection[11] in formulations. Literature survey also reported spectrophotometric[12], difference spectrophotometric[13], micellar liquid chromatography[14] and derivative spectrophotometry[15] methods for CLR with other drugs in pharmaceutical formulations. IMI and CLR are formulated together in the form of a tablet.…”

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confidence: 99%

Simultaneous spectrophotometric estimation of imipramine hydrochloride and chlordiazepoxide in tablets

Patel

2009

Indian J Pharm Sci

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A binary mixture of imipramine HCl and chlordiazepoxide was determined by three different spectrophotometric methods. The first method involved determination of imipramine HCl and chlordiazepoxide using the simultaneous equations and the second method involved absorbance ratio method. Imipramine has absorbance maxima at 251 nm, chlordiazepoxide has absorbance maxima at 264.5 nm and isoabsorptive point is at 220 nm in methanol. Linearity was obtained in the concentration ranges of 1-25 and 1-10 μg/ml for Imipramine HCL and Chlordiazepoxide, respectively. The third method involved determination of these two drugs using the first-derivative spectrophotometric technique at 219 and 231.5 nm over the concentration ranges of 1-20 and 2-24 μg/ml with mean accuracies 99.46±0.78 and 101.43±1.20%, respectively. These methods were successively applied to pharmaceutical formulations because no interferences from the tablet excipients were found. The suitability of these methods for the quantitative determination of the compounds was proved by validation.

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Assay of Chlordiazepoxide and Demoxepam in Chlordiazepoxide Formulations by Difference Spectrophotometry

Cited by 14 publications

References 17 publications

Difference spectrophotometric assay of nitrazepam in tablet formulations

Difference spectrophotometric assay of nitrazepam in tablet formulations

Stability-Indicating HPLC Method for Simultaneous Determination of Clidinium Bromide and Chlordiazepoxide in Combined Dosage Forms

Simultaneous spectrophotometric estimation of imipramine hydrochloride and chlordiazepoxide in tablets

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