Assay of Cefotaxime by High-Pressure-Liquid Chromatography

Bergan, Tom; Solberg, Rita

doi:10.1159/000237972

Cited by 30 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cefotaxime and its desacetyl metabolite were quantified in serum after trichloroacetic acid deproteinization and separation by C8 RP-HPLC (10). Peak heights at 310 nm were directly proportional to concentration over the range of 0.3 to 40 p.g/ml, and results agreed with those of a bioassay method.…”

Section: Methodssupporting

confidence: 57%

See 1 more Smart Citation

Recent analytical methods for cephalosporins in biological fluids

Toothaker¹,

Wright²,

Pachla³

1987

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Since 1980, RP chromatography has been the principal analytical technique used for cephalosporins. This technology offers selectivity, accuracy, and ease of use. Most of the methods rely on protein precipitation and, to a lesser extent, solid-phase isolation or extraction procedures. The proper selection of a method depends on the analytical constraints imposed by the overall objective of the study. For example, pharmacokinetic datum interpretation mandates that the method be validated and provide specific and accurate results. LC is the preferred technique, since it not only meets these specifications but may also distinguish between the drug and metabolites. Those chromatographic methods which quantify several different cephalosporins are not desirable for pharmacokinetic datum interpretation, since accuracy and precision are usually compromised in order that many different drugs may be quantified in a single analysis. The proper selection of sample preparation method is dependent on the presence of potential interferences and the acceptable lower limit of quantitation. Protein precipitation methods offer ease of sample preparation but may suffer from nonselectivity. Solid-phase isolation and extraction procedures may increase selectivity and improve the limit of quantitation. Although LC provides specific and accurate results, clinical laboratories may prefer to use the less specific methods for therapeutic drug monitoring. In this case, microbiological, enzymatic, and fluorimetric methods offer improved sample throughput but less specificity. However, these methods should not be used for drugs that may have a low margin of safety or if the patient is on multiple-antibiotic therapy. Future methods may involve incorporating solid-phase isolation columns to enhance the specificity of chromatographic, microbiological, enzymatic, and fluorescence methods. Advancements in microbore column technology may allow improvements in the selectivity and sensitivity of LC methods. Many investigators prefer to use simple protein precipitation procedures for sample preparation because of sample throughput constraints. However, advances in robotic sample preparation may allow the more cumbersome solid-phase isolation or extraction techniques to be used to improved sample throughput and specificity.

show abstract

Section: Methodssupporting

confidence: 57%

“…The mobile phase consisted of 35% methanol and 5 mM tetrabutylammonium phosphate, and the A280 was monitored. Calibration curves were rectilinear from 10 to 100 ,gIml, and recoveries were greater than 98%.…”

Section: Methodsmentioning

confidence: 96%

Recent analytical methods for cephalosporins in biological fluids

Toothaker¹,

Wright²,

Pachla³

1987

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…It has been shown that older cephalosporins with a 3~ acetoxymethyl substituent, e. g. cephalothin, are metabolised to the respective desacetyl compounds (2). The degradation of cefotaxime to desacetyl cefotaxime has also been observed in man and was simultaneously reported from other laboratories (3,4). The purpose of this paper is to report in detail on the concentrations of cefotaxime and its antimicrobially active metabolite in human bile, measured both by high performance liquid chromatography (HPLC) and by bioassay with a selective test organism after an i.v.…”

Section: Introductionmentioning

confidence: 83%

Cefotaxime and desacetyl cefotaxime in human bile

et al. 1983

View full text Add to dashboard Cite

“…Microbiological assays have the ad ditional disadvantage that they are usually less accurate than HPLC [3,4], Conse quently, we determined the antibiotic con centrations both by HPLC and by micro biological procedures.…”

Section: Comparison Between Microbiological and Hplc Assaysmentioning

confidence: 99%

Relationship between Pharmacokinetics and Bioavailability of Cefroxadine (CGP 9000) and Renal Function

Bergan¹,

Brodwall

Larsen

1983

Chemotherapy

Self Cite

View full text Add to dashboard Cite

The pharmacokinetics and bioavailability of cefroxadine were studied in 15 patients with different renal functions after administration of 0.5 g as oral capsules and as intravenous infusions. Microbiological assays by agar diffusion and high-pressure liquid chromatography may both be used for this agent since no metabolite can be found. The bioavailability is near 100% of the oral dose regardless of renal function. Urinary recovery varied from about 50% in renal glomerular filtration rates (GFR) of less than 7 ml/min to nearly 100% in normal renal function. The serum concentrations, serum elimination half-life and total body clearance were significantly influenced by reduced renal function. Nonrenal elimination occurred in reduced renal function; the maximum serum elimination half-life was 24.6 h. Dose modifications according to renal function are suggested with from three doses/24 h in normal renal function to one dose/24 h in patients with GFR of 10 ml/min. The relative distribution volume corresponded to approximately 30% of the body weight. Tubular secretion of cefroxadine took place. The concentrations in urine remained above 32 mg/l for 12 h in all subjects regardless of renal function.

show abstract

Assay of Cefotaxime by High-Pressure-Liquid Chromatography

Cited by 30 publications

References 8 publications

Recent analytical methods for cephalosporins in biological fluids

Recent analytical methods for cephalosporins in biological fluids

Cefotaxime and desacetyl cefotaxime in human bile

Relationship between Pharmacokinetics and Bioavailability of Cefroxadine (CGP 9000) and Renal Function

Contact Info

Product

Resources

About