Assay for Viruses and Mycoplasmas Using Serologically Specific Electron Microscopy

Derrick, K. S.; Brlansky, R. H.

doi:10.1094/phyto-66-815

Cited by 100 publications

(34 citation statements)

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“…Immunosorbent electron microscopy (ISEM) was also one of the methods first used in virology. This technique, first described by Derrick (1972Derrick ( , 1973 involves the coating of electron microscope grids with specific antibodies and incubating these grids on extracts from the test plants. The antibodies specifically trap virus particles onto the grid.…”

Section: Serological Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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Section: Serological Methodsmentioning

confidence: 99%

New Approaches to the Detection of Microbial Plant Pathogens

Chu

Waterhouse

Martín

et al. 1989

Biotechnology and Genetic Engineering Reviews

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“…ZYMV also induces the presence of tubular scroll-like cytoplasmic cylindrical inclusions (CIs) of type 1 according to the classification of Edwardson & Christie (1978) (Edwardson, 1992), but some isolates were found to induce CIs of types 3 and 4 (pinwheels, scrolls, bundles and laminated aggregates) (Petersen et al, 1991). Unambiguous identification of ZYMV particles in infected leaves can be achieved by immunosorbent electron microscopy (ISEM): virus particles are first trapped on a grid activated by antisera (Derrick, 1973) and subsequently decorated specifically by a homologous antiserum. Virus particles appear coated by a 'halo' of antibody molecules (Milne & Luisoni, 1977).…”

Section: Electron Microscopymentioning

confidence: 99%

Zucchini yellow mosaic virus

Desbiez¹,

Lecoq²

1997

Plant Pathology

221

172

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Zucchini yellow mosaic potyvirus (ZYMV), first isolated in Italy in 1973, described in 1981, and then identified in all continents within a decade, is one of the most economically important viruses of cucurbit crops. It is efficiently aphid-transmitted in a nonpersistent manner and it is also seed-borne in zucchini squash, which could have contributed to its rapid spread worldwide. Biological variability has been observed among ZYMV isolates, concerning host range, symptomatology and aphid transmissibility. More recent studies also revealed a serological and molecular variability. The survival of ZYMV in areas where cucurbits are not grown throughout the year remains to be elucidated, because very few natural over-wintering hosts have been identified so far. Partial control of ZYMV can be achieved by limiting transmission of the virus to the crops by aphids, using adapted cultural practices. Cross-protection with a mild strain has been shown to be effective against most ZYMV isolates. Resistance genes found in cucurbit germplasms are currently being introduced into cultivars with good agronomical characteristics. Pathogen-derived resistance strategies using the expression of ZYMV genes in transgenic plants have also been developed and appear promising. Nevertheless, the high biological variability of ZYMV justifies a careful evaluation of the deployment of genetic control strategies in order to increase their durability.

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“…Immunoelectron microscopical methods (e.g. Derrick & Brlansky, 1976;Milne & Lesemann, 1978;Roberts et al, 1980;Conti et al, 1980;Nicolaieff & van Regenmortel, 1980;Lesemann & Paul, 1980) have the great advantage of being able to be applied to tissue homogenates and of requiring very low quantities of virus and antiserum. Relationships can be studied in different ways: (i) by the differential trapping of virus particles on electron microscope grids coated with antisera to different viruses; (ii) by endpoint dilution of an antiserum which effectively coats (decorates) the virus particles; (iii) by the observation of a clumping reaction at different serum concentrations.…”

Section: Serology (A) Precipitin Tests Antigenic Analysis Of Isometrmentioning

confidence: 99%