Assay for Urinary Methylmalonic Acid by High-pressure Liquid Chromatography

Toyoshima, Shigeki; Saido, Hisako; Watanabe, Fumio; Miyatake, Kazutaka; Nakano, Yoshihisa

doi:10.1271/bbb.58.1882

Cited by 9 publications

(7 citation statements)

References 2 publications

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“…Vitamin B 12 deficiency causes multiple metabolic disorders (Weidemann et al 1970;Williams & Spray, 1971;Fehling et al 1978;Brass & Stabler, 1988), which appear to lead to severe growth retardation in rats. Toyoshima et al (1994) have demonstrated that an unusual accumulation of methylmalonic acid caused by vitamin B 12 deficiency disrupts normal cellular metabolism (especially metabolic inhibition of the Krebs cycle) in rat liver. To prevent the accumulation of the toxic methylmalonic acid, the vitamin B 12 taken up by hepatic cells of the vitamin B 12 -deficient rats would be immediately converted to ado-B 12 , which functions as the coenzyme of methylmalonyl-CoA mutase, catalysing the isomerization of L-methylmalonyl-CoA to succinyl-CoA.…”

Section: Resultsmentioning

confidence: 99%

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Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status

Takenaka¹,

Sugiyama²,

Ebara

et al. 2001

Br J Nutr

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To clarify the bioavailability of vitamin B 12 in lyophylized purple laver (nori; Porphyra yezoensis), total vitamin B 12 and vitamin B 12 analogue contents in the laver were determined, and the effects of feeding the laver to vitamin B 12 -deficient rats were investigated. The amount of total vitamin B 12 in the dried purple laver was estimated to be 54´5 and 58´6 (SE 5´3 and 7´5 respectively) mg/100 g dry weight by Lactobacillus bioassay and chemiluminescent assay with hog intrinsic factor respectively. The purple laver contained five types of biologically active vitamin B 12 compounds (cyano-, hydroxo-, sulfito-, adenosyl-and methylcobalamin), in which the vitamin B 12 coezymes (adenosyl-and methylcobalamin) comprised about 60 % of the total vitamin B 12 . When 9-week-old vitamin B 12 -deficient rats, which excreted substantial amounts of methylmalonic acid (71´7(SE 20´2) mmol/d) in urine, were fed the diet supplemented with dried purple laver (10 mg/kg diet) for 20 d, urinary methylmalonic acid excretion (as an index of vitamin B 12 deficiency) became undetectable and hepatic vitamin B 12 (especially adenosylcobalamin) levels were significantly increased. These results indicate that vitamin B 12 in dried purple laver is bioavailable to rats.Vitamin B 12 deficiency: Purple laver: Urinary methylmalonate excretion: Hepatic vitamin B 12 content

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Section: Resultsmentioning

confidence: 99%

“…The urine of the vitamin B 12 -deficient, vitamin B 12supplemented and purple laver-supplemented rats was sampled for 24 h in individual metabolism cages at days 0, 10 and 20 during the experiments. Urinary methylmalonic acid was assayed by HPLC, as described previously (Toyoshima et al 1994).…”

Section: Urinary Methylmalonic Acid Assaymentioning

confidence: 99%

Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status

Takenaka¹,

Sugiyama²,

Ebara

et al. 2001

Br J Nutr

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“…The urine of the vitamin B,,-deficient and control rats was sampled for 24 h in individual metabolic cages at the indicated times during the experiments. Urinary methylmalonic acid (MMA) was assayed by high performance liquid chromatography (HPLC) as described previously (Toyoshima et al, 1994). At 12 weeks old, serum was collected from the vitamin B ,,-deficient and B,,-supplemented rats to analyze…”

Section: Biochemical Analysismentioning

confidence: 99%

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Watanabe¹,

Ohkawa²,

Kasai³

et al. 2003

Clinical Genetics

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To evaluate the role of vitamin B,, on spermatogenesis, the effects of dietary vitamin B,, deficiency on sperm maturation in developing rat fetuses and young growing rats were examined. The vitamin B,,-deficient diet was given to all the animals for three different periods: whole period (gestation to mature), gestation period (gestation to weaning), or immature period (3-12 weeks postnatal). Sperm examination revealed that the sperm count was markedly lower in male progeny (Fl) that were vitamin B,,-deficient during the whole period. In addition, a significantly higher number of abnormal sperm, such as tailless and amorphous sperm, was observed. In male rats that were vitamin B,,-deficient during the immature period, the incidence of abnormal sperms was 14.4% and 4.8% for tailless and short tail, respectively. The motion rates, such as path velocity and straight line velocity, were decreased to 20-40% of the control value in rats that were vitamin B,,-deficient both during the whole and gestation periods. However, no effects of vitamin B,, deficiency on sperm motility were observed during the immature and mature periods. From these findings, we suggest that dietary vitamin B,, deficiency during pregnancy may induce irreversible damage in the germ cells of embryos and affect the maturation of spermatozoa.Abbreviations used in this paper: B,,, vitamin BI2 (cobalamin); CH3-BI2 (Me-B,,), mecobalamin; CN-B,,, cyanocobalamin; MMA, methylmalonic acid; HPLC, high performance liquid chromatography

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“…The urine of the B 12 deficient, cyano-B12-supplemented, and tea drink-supplemented rats was sampled for 24 h in individual methabolic cages at weeks 0, 1, 2, and 6 during the experiments. Urinary methylmalonic acid was assayed by HPLC as described previously (6).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Corrinoid Compounds from a Japanese Black Tea (Batabata-cha) Fermented by Bacteria

Kittaka-Katsura

Ebara

Watanabe

et al. 2004

J. Agric. Food Chem.

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A Japanese fermented black tea (Batabata-cha) contained a considerable amount of vitamin B(12) (456 +/- 39 ng per 100 g dry tea leaves and 2.0 +/- 0.3 ng per 100 mL of tea drink). A corrinoid compound was partially purified and characterized from the tea leaves. The patterns of the purified compound by the silica gel 60 thin-layer chromatography and C18 reversed phased high-performance liquid chromatography were identical to those of authentic vitamin B(12). When 20 week old vitamin B(12) deficient rats, which excreted substantial amounts (about 250 mg/day) of methylmalonic acid in urine as an index of vitamin B(12) deficiency, were fed the tea drink (50 mL/day, 1 ng of vitamin B(12)) for 6 weeks, urinary methylmalonic acid excretion (169 +/- 29 mg/day) of the tea drink-supplemented 26 week old rats decreased significantly relative to that (250 +/- 32 mg/day) of the deficient rats. The results indicate that the vitamin B(12) found in the fermented black tea is bioavailable in mammals.

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Assay for Urinary Methylmalonic Acid by High-pressure Liquid Chromatography

Cited by 9 publications

References 2 publications

Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status

Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Characterization of Corrinoid Compounds from a Japanese Black Tea (Batabata-cha) Fermented by Bacteria

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Assay for Urinary Methylmalonic Acid by High-pressure Liquid Chromatography

Cited by 9 publications

References 2 publications

Feeding dried purple laver (nori) to vitamin B12-deficient rats significantly improves vitamin B12 status

Feeding dried purple laver (nori) to vitamin B12-deficient rats significantly improves vitamin B12 status

The effects of dietary vitamin B12 deficiency on sperm maturation in developing and growing male rats

Characterization of Corrinoid Compounds from a Japanese Black Tea (Batabata-cha) Fermented by Bacteria

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Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status

Feeding dried purple laver (nori) to vitamin B₁₂-deficient rats significantly improves vitamin B₁₂ status