Asperyellone pretreatment protects HaCaT cells from UVB irradiation induced oxidative damages: Assessment under in vitro and in vivo conditions and at molecular level

Abstract

The present study explores the UVB protective role of Asperyellone (AY), a secondary metabolite of Aspergillus niger strain AN01. The in vitro UVB protective efficacy of AY was studied using the Human Epidermal keratinocytes cells (HaCaT) cell line. The results suggest the appreciable scavenging of UVBinduced reactive oxygen species in the AY-pretreated cells compared with UVB control. Experimental results on the antioxidant enzymes (Catalase, SOD, LPO, and GPx) profile, histochemical, and molecular analyses support the UVB protective effect of AY in HaCaT cells. Further, the in vivo UVB protective efficacy of AY was studied using animal models and compared with that of commercially available UVB protective agents. Physical, biochemical, and molecular analyses of skin samples emphasized the UVB protective role of AY. Thus, the important beneficial effects of AY have been explored in the present study. K E Y W O R D S apoptotic proteins, Aspergillus niger, asperyellone (AY), epidermal thickening, human epidermal keratinocytes cells (HaCaT) cells, UVB protection J Cell Biochem. 2019;120:10715-10725.wileyonlinelibrary.com/journal/jcb

Epigallocatechin gallate enhances human lens epithelial cell survival after UVB irradiation via the mitochondrial signaling pathway

“…Previous studies have suggested that UVB irradiation can increase the concentration of H 2 O 2 and hydroxyl free radicals. UVB irradiation has also been reported to inhibit the activities of CAT, SOD and GSH-Px, and reduce the concentration of GSH, thus causing oxidative damage to cells ( 24 , 25 , 45 ). Previous studies have revealed that EGCG can protect against light ( 12 , 13 ), and that it can also reduce the oxidative damage caused by UVB irradiation by increasing CAT, SOD, GSH-Px and GSH activities ( 10 , 46 ).…”

Aspergillus niger as a Secondary Metabolite Factory

“…Asperyellone ( 147) was the common product of strains NRRL-3 and CFTRI 1105 (Jefferson, 1967;Chidananda et al, 2008) and exhibited inhibitory effect on lipoxygenase and human platelet aggregation (Rao et al, 2002), UVB protection (Santhakumaran et al, 2019), and antifungal activity (Ayer et al, 1996). In addition to γ-pyridone (86), five highly oxygenated pyranoquinones (86, 149, and 151-154) were detected in SMs of strain T1 by activation of the aza gene cluster (Zabala et al, 2012) (Figure 8).…”