Aspergillus spp. and Bacillus spp. as Growth Promoters in Cotton Plants Under Greenhouse Conditions

Diaz, Paola Andrea Escobar; Aguirre-Gil, Oniel Jeremías; Barbosa, Carlos Henrique; Desoignies, Nicolas; Rigobelo, Everlon Cid

doi:10.3389/fsufs.2021.709267

Cited by 8 publications

(6 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the B. subtilis bacterium used in the present study has several plant growth-promoting characteristics, such as biological nitrogen fixation, phosphorus solubilization, and indole acetic acid production, as has been demonstrated in previous studies in soybean, corn and cotton crops (Lobo et al, 2019;dos Santos et al, 2021;Escobar Diaz et al, 2021a). Different plant organs have different ways of being colonized by microorganisms (Compant et al, 2021).…”

Section: Discussionmentioning

confidence: 57%

Inoculum Concentration and Mineral Fertilization: Effects on the Endophytic Microbiome of Soybean

Santos

Cueva-Yesquén

Fantinatti-Garboggini

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Soybean crops are of great economic importance worldwide and in Brazil. This crop is a commodity that provides large amounts of financial resources to the country. Soybean productivity is influenced by several biotic and abiotic factors, and most of these factors cannot be controlled by agricultural practices. Due to the soybean cultivars used and their required yields, the soybean crop, similar to other agriculturally important crops, requires large amounts of mineral fertilizers. There are several microorganisms that colonize soybean plant roots without causing symptoms or damage. These microorganisms that colonize plant tissues are called endophytes and can often promote plant growth and development. Little is known about the factors that influence endophyticism. The aim of the present study was to evaluate whether Bacillus subtilis inoculant concentrations and levels of mineral fertilization recommended for the crop have any influence on the endophytic microbiome of soybean plant roots. The results show that B. subtilis inoculations did not affect the endophytic community of the roots; however, the evaluation of the microbial community structure according to the alpha diversity metrics observed richness, Chao1 index, Shannon index and Simpson index showed that microbial diversity of endophytes was higher at fertilization levels of 50 and 100%, with a significant difference (p < 0.05) between 0 and 50% and 0 and 100% fertilization.

show abstract

Section: Discussionmentioning

confidence: 57%

Inoculum Concentration and Mineral Fertilization: Effects on the Endophytic Microbiome of Soybean

Santos

Cueva-Yesquén

Fantinatti-Garboggini

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this regard, A. sydowii is a halophilic fungus commonly found in different marine ecosystems and is considered a model organism for studying molecular adaptations of filamentous fungi to hyperosmolarity [ 37 ]. Recent studies showed that A. sydowii enhances plant growth by stimulating phosphorus solubilization in maize [ 38 ] and promoting higher phosphorus absorption by cotton plants [ 39 ]. The marine A. versicolor is a siderophore producer [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Salinity and Genotype on the Rhizospheric Mycobiomes in Date Palm Seedlings

Yaish,

Al-Busaidi,

Glick

et al. 2024

Biology

View full text Add to dashboard Cite

Salinity severely affects the health and productivity of plants, with root-associated microbes, including fungi, potentially playing a crucial role in mitigating this effect and promoting plant health. This study employed metagenomics to investigate differences in the structures of the epiphyte mycobiomes in the rhizospheres of seedlings of two distinct date palm cultivars with contrasting salinity tolerances, the susceptible cultivar, ‘Zabad’, and the tolerant cultivar, ‘Umsila’. Next-generation sequencing (NGS) of the internal transcribed spacer (ITS) rRNA was utilized as a DNA barcoding tool. The sequencing of 12 mycobiome libraries yielded 905,198 raw sequences of 268,829 high-quality reads that coded for 135 unique and annotatable operational taxonomic units (OTUs). An OTU analysis revealed differences in the rhizofungal community structures between the treatments regardless of genotype, and non-metric dimensional scaling (N-MDS) analyses demonstrated distinct separations between the cultivars under saline stress. However, these differences were not detected under the control environmental conditions, i.e., no salinity. The rhizospheric fungal community included four phyla (Ascomycota, Basidiomycota, Chytridiomycota, and Mucoromycota), with differences in the abundances of Aspergillus, Clonostachys, and Fusarium genera in response to salinity, regardless of the genotype. Differential pairwise comparisons showed that Fusarium falciforme-solani and Aspergillus sydowii-versicolor increased in abundance under saline conditions, providing potential future in vitro isolation guidelines for plant growth-promoting fungi. This study highlights the intricate dynamics of the rhizosphere microbial communities in date palms and their responses to salt stress. Additionally, we found no support for the hypothesis that indigenous epiphytic fungal communities are significantly involved in salinity tolerance in date palms.

show abstract

“…Bacillus subtilis used in this study has several abilities to promote plant growth, such as the synthesis of phytohormones, biological nitrogen fixation, promotion of root and shoot development and increased productivity in several crops, such as soybean, maize, cotton and sugarcane ( dos Santos et al, 2018 ; Lobo et al, 2019 ; Shastri et al, 2020 ; Dos Santos et al, 2021 ; Escobar Diaz et al, 2021 ). However, in previous studies, the B. subtilis concentration used was 1 × 10 9 CFU ml −1 , and the fertilization dose was 100% the recommended dose for each crop.…”

Section: Discussionmentioning

confidence: 99%

“…However, these studies did not evaluate the effect of high inoculum concentrations on plant growth. Escobar Diaz et al (2021) assessed the impact of different inoculum concentrations, including B. subtilis , on cotton crops and did not find a difference between yield crops when the plants received the inoculum at 1 × 10 4 and 1 × 10 10 CFU ml −1 . However, these authors evaluated the effect of different inoculum concentrations at 100% of the fertilization dose, only.…”

Section: Discussionmentioning

confidence: 99%

Effects of Chemical Fertilization and Microbial Inoculum on Bacillus subtilis Colonization in Soybean and Maize Plants

Bueno

Santos

Buzo³

et al. 2022

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Plant growth-promoting endophytic microorganisms in agriculture have been expanding in Brazil and are an excellent strategy to face the challenges of current agriculture, such as reducing production costs with fewer environmental impacts, without detriment to productivity. However, little is known about the factors that can affect the colonization of endophytic such as inoculant concentration and mineral fertilization. The present study aimed to evaluate the influence of these factors on soybean and maize crops and found that for soybean crops, the highest Bacillus subtilis concentration of 1 × 104 and 1 × 1010 CFU ml−1 promoted the highest number of recovered bacteria, when there was no mineral fertilization. However, mineral fertilization limited the number of recovered bacteria, suggesting that mineral fertilization interferes with endophytic colonization. For maize crops, the highest number of recovered bacteria occurred from the concentration of 1 × 106 CFU ml−1, not differing from the highest concentrations. A mineral fertilization dose of 25% promoted the greatest B. subtilis recovery compared to the other treatments. Regarding plant development, the highest microbial inoculum concentrations did not necessarily promote greater positive growth promotion effects compared to the concentration of 1 × 104 CFU ml−1 for both crops. The results also suggest that the higher number of endophytic bacteria recovered in the plant does not necessarily affect plant growth in the same proportion. For soybean plants, there is a strong tendency that with the increase in the B. subtilis inoculant concentration, the need for mineral fertilization doses to achieve the same plant development is consequently increased, and inoculations with 1 × 105 and 1 × 106 CFU ml−1 with fertilization doses between 44% and 62% are the ideal combinations for greater plant development. In maize plants, the best growth promotion response (height) was obtained using inoculation concentration of 1 × 102 and 1 × 1010 CFU ml−1, increasing according to the increase in fertilization doses. The findings suggest, for soybean crop, that these high inoculum concentrations required more photosynthetic metabolites from the plants and more nutrients from the soil. Thus, the need for mineral fertilization for plant growth must be increased.

show abstract

Aspergillus spp. and Bacillus spp. as Growth Promoters in Cotton Plants Under Greenhouse Conditions

Cited by 8 publications

References 45 publications

Inoculum Concentration and Mineral Fertilization: Effects on the Endophytic Microbiome of Soybean

Inoculum Concentration and Mineral Fertilization: Effects on the Endophytic Microbiome of Soybean

The Effects of Salinity and Genotype on the Rhizospheric Mycobiomes in Date Palm Seedlings

Effects of Chemical Fertilization and Microbial Inoculum on Bacillus subtilis Colonization in Soybean and Maize Plants

Contact Info

Product

Resources

About