Aspergillus section Flavi and aflatoxins in Algerian wheat and derived products

Riba, Amar; Bouras, Noureddine; Mokrane, Salim; Mathieu, Florence; Lebrihi, Ahmed; Sabaou, Nasserdine

doi:10.1016/j.fct.2010.07.005

Cited by 103 publications

(81 citation statements)

References 32 publications

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“…The molecular identification of the obtained monosporic isolates revealed that they were all A. flavus (Table 1). The occurrence of A. flavus and not of A. parasiticus was also reported in wheat grown and consumed in Lebanon (Joubrane et al, 2011) and Algeria (Riba et al, 2010).…”

Section: Discussionmentioning

confidence: 97%

Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat

et al. 2017

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Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code enzymes of the aflatoxins synthesis pathway. Some of the isolates were confirmed to be aflatoxin producers and several of them presented a genetic profile of aflatoxin synthesis. The obtained results demonstrated that Bulgur wheat A. flavus contamination is concerning. Keywords: Aspergillus flavus; aflatoxins; Bulgur wheat; PCR.Practical Application: Aflatoxicosis associated with consumption of contaminated food is a major concern due to the risk of liver cancer and other diseases. The presence of aflatoxigenic Aspergillus needs to be monitored to evaluate the potential for aflatoxin contamination in food. This study presents methods of screening for aflatoxigenic Aspergillus in commercial Bulgur wheat. In addition, this work offers methods of isolation and identification of the Aspergillus species present in food and methods for testing the aflatoxigenic potential of the isolates.

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Section: Discussionmentioning

confidence: 97%

Occurrence of toxigenic Aspergillus flavus in commercial Bulgur wheat

et al. 2017

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“…The most common genera are Aspergillus, Penicillium, Fusarium, and Alternaria isolated from wheat grain in Saudi Arabia (Al-Hazmi, 2010;Gashgari et al, 2010). The contamination of cereal grains by fungi is a worldwide problem, where there are several reports documenting the presence of these fungi in samples from the United States (Cleveland et al, 2003), Argentina (Roige et al, 2009), Spain (Sardiñas et al, 2011), and Algeria (Riba et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

Alwadai

Al‐Othman

Mahmoud³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened for their ability to produce aflatoxins using HPLC. Thirteen isolates produced aflatoxins ranging from 0.5 to 2.6 µg/kg. Inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) molecular markers were used, with the aim of genetically characterizing strains of A. flavus to discriminate between aflatoxigenic and non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population, useful for genetic characterization. Clustering based on RAPD and ISSR dendograms was unrelated to geographic origin. RAPD and ISSR markers were not suitable to discriminate aflatoxigenic and nonaflatoxigenic isolates, but ISSR primers were better compared to RAPD.

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“…While 12 strains from peanut and 5 from wheat were high producers. Several studies reported the aflatoxigenic potential of different A. flavus strains collected from various seeds and grains all over the world (Riba et al, 2010, Ezekiel et al, 2012, Kana et al, 2013and Fakruddin et al, 2015.…”

Section: Aflatoxin Productionmentioning

confidence: 99%