Aspergillus fumigatus Isolated from Blood Samples of a Patient with Pulmonary Aspergilloma after Embolization.

Abstract

Aspergillus DNAwas detected by PCR in the serum sample of a 78-year-old man and galactomannan antigen ofAspergillus by sandwich ELISA was found. However, the infiltrative hyphae were not detected by the histopathologic examination of the lung. He developed hemoptysis, which required embolization of bronchial arteries. Aspergillus fumigatus was isolated from blood samples after embolization by the lysis centrifugation method. To our knowledge, this is probably the first case in which Aspergillus spp. has been isolated from the systemic circulatory blood in a patient with pulmonary aspergilloma after embolization.

Isolation of Neosartorya pseudofischeri from Blood: First Hint of Pulmonary Aspergillosis

“…from blood is usually related to a central venous catheter infection, to cardiac surgery, or, for neutropenic patients, to pulmonary infection. Positive blood cultures have been reported for one case of pulmonary aspergilloma (10). The rarity of the isolation of Aspergillus spp.…”

Polymerase chain reaction in the diagnosis of invasive aspergillosis

“…Fungemia caused by Aspergillus species is worth of publication because there are limited data in the literature. 5,[8][9][10][11][12][13] Blood cultures are rarely positive in disseminated aspergillosis. 8,14 PCR assay is more sensitive for the detection of Aspergillus in blood when compared with culture.…”

“…[24][25][26] Although the PCR assay has lots of superiority to the other methods, increased sensitivity may cause some limitations. Seki et al 5 suggested that extracting of Aspergillus DNA from blood may be the result of the circulation of fungal cell or DNA particles in blood rather than fungemia. Because of this limitation, DNAemia cannot be ruled out in this case.…”

“…Polymerase chain reaction (PCR) technique has also been suggested as a mode of detecting the infection; but the diagnostic value of the PCR remains to be established in prospective studies. 4,5 In this report, an immuncompromised patient with IPA diagnosed and monitored by PCR is presented. The PCR protocol for the isolation of the DNA from the blood samples is also described.…”

Pulmoner Aspergillomada Uzun Dönem Antifungal Tedavi Cerrahiye Alternatif Olabilir mi?

“…Hemoptysis is the major life-threatening acute clinical condition caused by PA. There is also some risk of developing invasive aspergillosis or fungal septicemia, which have even higher morbidity and mortality (5,6). Treatment and follow-up of patients with PA still lack a well-defined evidence-based algorithm.…”