Aspergillus fumigatus conidia stimulate lung epithelial cells (TC-1 JHU-1) to produce IL-12, IFNγ, IL-13 and IL-17 cytokines: Modulatory effect of propolis extract

Khosravi, Ali Reza; Alheidary, S.; Nikaein, Donya; Asghari, Narjes

doi:10.1016/j.mycmed.2018.09.006

Cited by 18 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase of IL-5 and GM-CSF will promote eosinophil recruitment, thus exacerbate airway damage 21 . In addition, Khosravi AR et al have found that airway epithelial cells exposed to aspergillus fumigatus spores produced more IFN-γ, which lead to brief periods of increased IFN-γ within the body to promote aspergillus clearance and play a defensive role 22,23 . In our study, ABPA patients with elevated serum CEA levels have a higher level of Th2 cytokines (IL-4 and IL-5),GM-CSF and IFN-γ,which indicate that a more intensely in ammatory response occurrence in vivo environment of patients.…”

Section: Discussionmentioning

confidence: 99%

Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?

Yin

Jiang

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Allergic bronchopulmonary aspergillus (ABPA) is a complex non-infectious pulmonary benign disease caused by immune response against to aspergillus. Carcinoembryonic antigen (CEA) is a tumor marker but also elevated in some benign diseases. Few studies on ABPA with elevated serum CEA levels have been reported. Objects: This study aims to comb the clinical characters of ABPA with elevated serum CEA. Methods: 20 patients diagnosed as ABPA were divided into two groups (CEA normal and CEA elevated) for retrospective analysis. The eosinophil count and ratio, IgE level in the pretherapy and post-treatment were compared. Serum samples of patients with ABPA (n = 20) and asthma (n = 20), healthy controls (n=20) were collected. Levels of cytokines (IL-4, IL-5, GM-CSF, IFN-γ) were analyzed by enzyme-linked immunosorbent assay. Results: We found that in ABPA patients with normal serum CEA levels, eosinophil counts and IgE levels decreased more obviously after treatment. Besides, we established higher serum levels of IL-4, IL-5, GM-CSF and IFN-γ in ABPA patients with elevated serum CEA levels.Conclusion: For the ABPA patients with elevated serum CEA levels, CEA may serve as a monitoring indicator for severity and treatment efficacy of ABPA.

show abstract

Section: Discussionmentioning

confidence: 99%

Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?

Yin

Jiang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Perhaps the most interesting results from this study were for cytokine IL-17. IL-17 is expressed by Th17 cells, as well as NK cells, and neutrophils, and has been reported in other species to be expressed within pulmonary epithelium [22, 25]. IL-17 is a secreted cytokine that binds pulmonary epithelial cells inducing mucin production and stimulates neutrophil recruitment to the site of infection [26].…”

Section: Discussionmentioning

confidence: 99%

“…For five of the immunomarker antibodies (anti-CD3, anti-CD20, anti-CD79a, anti-CD163, and anti-Iba-1), the ROI included bronchi/bronchioles and the surrounding tissue including associated immune tissue (BALT, immune cell “cuffing”), but excluded bronchial cartilage and surrounding alveoli; these regions are referred to simply as “bronchiolar” ROIs (Image A in S1 Fig). Since IL-17 is produced by both immune cells and respiratory epithelial cells [22], the bronchiolar ROIs were each subdivided into 2 ROIs for quantifying IL-17 immunolabeling: bronchiolar epithelium and bronchiolar ROI’s excluding the epithelium, referred to as “epithelium” and “BALT”, respectively. Macrophage immunomarkers (CD163 and Iba1) and the IL-17 cytokine marker were also evaluated in alveoli and alveolar walls, this “alveolar” ROI was identified by selecting the whole lung tissue section and excluding bronchiolar ROIs (Image B in S1 Fig).…”

Section: Methodsmentioning

confidence: 99%

Differential pulmonary immunopathologic response of domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) to Mycoplasma ovipneumoniae infection: a retrospective study

Grossman

Schneider

Knowles

et al. 2019

Preprint

View full text Add to dashboard Cite

Mycoplasma ovipneumoniae is a respiratory pathogen that can impact domestic sheep (Ovis aries; DS) and bighorn sheep (Ovis canadensis; BHS). Experimental and field data have indicated BHS are more susceptible than DS to developing polymicrobial pneumonia associated with Mycoplasma ovipneumoniae infection. We hypothesized that DS and BHS have a differential immunopathologic pulmonary response to M. ovipneumoniae infection. A retrospective study was performed using formalin-fixed, paraffin-embedded (FFPE) lung tissue from DS and BHS without and with M. ovipneumoniae detected in the lung tissue (n=8 per group). While each M. ovipneumoniae positive lung sample had microscopic changes typical of infection, including hyperplasia of intrapulmonary bronchus-associated lymphoid tissue (BALT) and respiratory epithelium, DS exhibited a more robust and well-organized BALT formation as compared to BHS. Immunohistochemistry was performed with antibodies reactive in FFPE tissues and specific for leukocyte and cytokine markers: T cell marker CD3, B cell markers CD20 and CD79a, macrophage markers CD163 and Iba1, and cytokine IL-17. Digital analysis was used to quantitate chromogen deposition in regions of interest (ROIs), including alveolar and bronchiolar areas, and bronchiolar subregions (epithelium and BALT). Main effects and interaction of species and infection status were analyzed by beta regression and Bonferroni corrections were performed on pairwise comparisons (PBon<0.05 significance). Significant species differences were identified for bronchiolar CD3 (PBon=0.0023) and CD163 (PBon=0.0224), alveolar CD163 (PBon=0.0057), and for IL-17 in each of the ROIs (alveolar: PBon=0.0009; BALT: PBon=0.0083; epithelium: PBon=0.0007). Infected BHS had a higher abundance of bronchiolar CD3 (PBon=0.0005) and CD163 (PBon=0.0162), and alveolar CD163 (PBon=0.0073). While IL-17 significantly increased with infection in BHS BALT (PBon=0.0179) and alveolar (0.0006) ROIs, abundance in DS showed an insignificant decrease in these ROIs and a significant decrease in epithelial abundance (PBon=0.0019). These findings support the hypothesis that DS and BHS have a differential immunopathologic response to M. ovipneumoniae infection.

show abstract

“…An in vitro study by Khosravi et al (2018) evaluated the effects of the ethanol extract of propolis collected from the hives of Apis mellifera L. bees of southern Iran on cytokine production in murine lung epithelial cells TC-1 JHU-1 induced with a fungal FIGURE 1 | Different types of propolis extracts have shown anti-allergic effects against allergic inflammation, asthma, allergic rhinitis, and atopic dermatitis. Brazilian green propolis is the most promising remedy for allergic diseases as it has been demonstrated to inhibit several pathophysiological mechanisms in allergy.…”

Section: Allergen-induced Allergic Inflammationmentioning

confidence: 99%

“…IL: Interleukin; TSLP: thymic stromal lymphopoietin; Th2: T helper 2; IgE: Immunoglobulin E; CysLTs: cysteinyl leukotrienes; MCP-1: monocyte chemoattractant protein-1. allergen, Aspergillus fumigatus conidia (Khosravi et al, 2018) (Table 1A). Propolis ethanol extract treatment has been shown to suppress the production of IL-13 and IL-17 pro-inflammatory cytokines and increase IL-12 production.…”

Section: Allergen-induced Allergic Inflammationmentioning

confidence: 99%

Anti-Allergic Properties of Propolis: Evidence From Preclinical and Clinical Studies

Liew

Kamise²,

Ong³

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Allergic diseases are a global health burden with increasing prevalence. Side effects of available medications (antihistamines and steroids), lack of patients’ perceived effectiveness and high cost of biologic therapies (omalizumab) are challenges to the clinical management of allergic diseases. As allergy symptoms persist for a long time, complementary and alternative medicine (CAM) such as propolis may be considered a potential prophylactic or therapeutic option to avoid long-term medication use. Propolis is a natural resinous substance produced by bees. Although propolis is well known to possess antioxidant, antimicrobial, and anticancer properties, its anti-allergic potential is not fully explored. Several preclinical studies demonstrated the therapeutic effects of propolis extracts against allergic inflammation, asthma, allergic rhinitis, atopic dermatitis, and food allergy, which may be partly attributed to their inhibitory effects on the activation of mast cells and basophils. Clinically, the consumption of propolis as a supplement or an adjunct therapy is safe and attenuates various pathological conditions in asthma. Such an approach may be adopted for atopic dermatitis and allergic rhinitis. Although flavonoids (chrysin, kaempferol, galangin, and pinocembrin) and cinnamic acid derivatives (artepillin C and caffeic acid phenethyl ester) can contribute to the anti-allergic activities, they may not be present in all propolis samples due to variations in the chemical composition. Future studies should relate the anti-allergic activity of propolis with its chemical contents. This mini-review summarizes and discusses existing preclinical and clinical studies reporting the anti-allergic activities of propolis to provide insights into its potential applications in allergic diseases.

show abstract

Aspergillus fumigatus conidia stimulate lung epithelial cells (TC-1 JHU-1) to produce IL-12, IFNγ, IL-13 and IL-17 cytokines: Modulatory effect of propolis extract

Cited by 18 publications

References 36 publications

Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?

Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?

Differential pulmonary immunopathologic response of domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) to Mycoplasma ovipneumoniae infection: a retrospective study

Anti-Allergic Properties of Propolis: Evidence From Preclinical and Clinical Studies

Contact Info

Product

Resources

About