Aspects of spermatogenesis and microscopic testicular morphology in Greater Rhea, Rhea americana (Linnaeus, 1758)

Carvalho, Saulo Fernandes Mano de; Sabóia‐Morais, Simone Maria Teixeira de; Freneau, Gustavo Eduardo

doi:10.1590/s0100-736x2016001000019

Cited by 6 publications

(6 citation statements)

References 28 publications

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“…One of the most important findings was the presence of leptotene primary spermatocytes in all stages of spermiogenesis and their long life span. These results are in accordance with previous reports in the duck [36], guinea fowl [35], Japanese quail [16] and greater Rhea [38] where leptotene, pachytene and zygotene primary spermatocytes were observed in the seminiferous epithelium of the relevant birds. Secondary spermatocytes are rarely observed in the seminiferous epithelium because their life span is very short [39].…”

Section: Discussionsupporting

confidence: 93%

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Akhtar

Ahmad

Mustafa

et al. 2020

Animals

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The past three decades revolutionized the goose industry in the world. China holds the world’s largest goose breeds stock by 95% of the global total. To optimize the goose industry and cope with ever increasing poultry meat and egg demands, there is a dire need to focus on reproduction, as most geese breeds exhibit poor reproductive performance. The present study was conducted with the aim to add a contribution in the goose industry and research by the histological visualizing step wise development of germ cells during spermatogenesis by microscopy and a histological technique. Yangzhou goose is a synthetic breed developed by using local goose germplasm resources of China. It is popular in the Chinese goose industry due to high productivity and adaptability. This research evaluated the steps of spermiogenesis and stages along with morphological changes in the seminiferous epithelium in Yangzhou goose ganders. For the assessment of various stages of the seminiferous epithelium cycle, testis sections were embedded in molten paraffin wax. The initial steps of spermiogenesis were depicted by changes in acrosomic granules, whereas further stages were identified by nuclear morphological changes. Ten steps of spermiogenesis and nine stages of seminiferous epithelium were identified. Four types of spermatogonia Ad, Ap1, Ap2 and B were recognized. The results depicted a clear variation in the diameter of seminiferous tubules (ST), epithelium height (EH), luminal tubular diameter (LD), number of seminiferous tubules per field and the Johnsen score. Microscopy indicated that the stages of seminiferous epithelium were similar to other birds and mammals and the ST diameter, EH, LD and Johnsen score are positively correlated while the number of seminiferous tubules per field is negatively correlated with the ST diameter, EH, LD and Johnsen score. Fertility in Yangzhou ganders can further be improved by visualizing the histological development of germs cells in testis tissues during spermatogenesis after onset of breeding season and maturity. Our results suggest that Yangzhou ganders reach complete sexual maturity at 227 days of age.

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Section: Discussionsupporting

confidence: 93%

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Akhtar

Ahmad

Mustafa

et al. 2020

Animals

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“…Two defined seasons were observed (dry and cold; and wet and hot). Climate data can be reviewed in more detail in FRENEAU et al (2016). All animals were subjected to equal handling and were fed commercial pelleted rations that contained the following: calcium (max.)…”

Section: Location and Animalsmentioning

confidence: 99%

Morphological aspects of epididymal microscopy and rete testis in greater Rhea Americana

2021

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The purpose of this research was to study the histology and describe the microscopy findings of the epididymis epithelium of greater Rhea americana at three time periods: November 2005 (n=14), December 2006 (n= 20), and May 2007 (n= 20), to observe and compare the differences that occurred. We studied the epididymis from 54 rheas, bred in Santa Maria, Rio Grande do Sul, Brazil. The epididymis were collected during commercial slaughter and fixed in bouin. Optical microscopy was used to measure the cellular structure, types of cells, tubules, and stereological values like the epididymis epithelium diameters, lumen, thickness, and relative volume of the tissue structure. Additionally, electron microscopy was studied. In December 2006 and May 2007, the means of the epididymis tubular diameter were: 79.1 and 58.1 µm, epithelium thickness: 24.0 and 52.2 µm, and lumen diameter: 55.0 and 5.8 µm, respectively. Regarding the volumetric proportion, we reported the following values: epithelium volume 36.2 and 80.4%, lumen without spermatozoon 19.6 and 3.0%, lumen with spermatozoon 5.4 and 0.0%, interstitium 35.4 and 12.0%, blood vessels 3.5 and 4.6%, structures in cellular superficies 1.4 and 0%, lamina 1.4 and 3.2%, and artifacts 0.3 and 1.3%, respectively. The epididymis ducts had a circular form in transverse sections with spermatozoon only in November 2005 and December 2006. The Rhea’s epididymis morphology was found to be similar to ostriches, roosters, and Japanese quail. Here, we present data from stereological microscopy (tubular diameter, epithelium thickness, and lumen diameter), volumetric proportion (epithelium, lumen without spermatozoon, lumen with spermatozoon, interstitium, blood vessels, structures in cellular superficies; cilium, estereocilium, and lamina) in this species during the repose and sexual activity period (reproductive season).

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“…The testicular capsule is composed of two main layers, the outer thin layer tunica serosa, and the inner thick connective tissue layer tunica albuginea (Maekawa et al, 1996; Ozegbe et al, 2008). A poorly differentiated innermost layer, tunica vasculosa, is also found in mammals and birds (Freneau et al, 2016). The tunica serosa contains secretory cells, which are mostly squamous or occasionally cuboidal; those secrete lubricating serous fluid and regulate testicular secretion (Aire & Ozegbe, 2007).…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Role of the testicular capsule in seasonal modulation of the testis

Pandey,

Mohanty

2023

J Exp Zool Pt A

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While the seasonal testicular cycle has been well studied regarding internal components, no attention has been given to the testicular capsule (tunica albuginea and tunica serosa). This study elucidated the structure‐function modulations of intra‐testicular functions by its capsule in the finch red munia (Amandava amandava) during the annual testicular cycle. The birds were studied during breeding (preparatory and breeding) and nonbreeding (regressive and quiescent) reproductive phases using hematoxylin‐eosin and acridine orange‐ethidium bromide capsule staining, hormonal ELISA (LH and testosterone) and immunohistochemical expression of neuropeptides (GnRH, GnIH) and androgen receptor (AR). The thickness of the tunica albuginea was significantly increased with multiple myoid layers during the nonbreeding phases (p < 0.05). The thickness of the tunica serosa was not altered, although characteristics and distribution of squamous cells showed significant seasonal alterations. Immunoreactive (‐ir) AR and GnIH cells were differentially localized on both layers of the capsule. Strong AR‐ir cells on tunica serosa during breeding phases showed increased expression of the receptor; a significant increase in plasma LH and testosterone was also observed during the breeding cycle (p < 0.01). Contrarily, intense GnIH‐ir cells on both the capsular layers peaked during testicular regression. Differential structural alterations of the testicular capsule provide mechanical support and help maintain internal homeostasis in tune with changing seasons. The seasonal expressions and alterations of reproduction‐related receptors, hormones, and neuropeptides provide evidence for the potential regulatory roles of the capsule in the peripheral modulation of intratesticular functions.

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Aspects of spermatogenesis and microscopic testicular morphology in Greater Rhea, Rhea americana (Linnaeus, 1758)

Cited by 6 publications

References 28 publications

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Morphological aspects of epididymal microscopy and rete testis in greater Rhea Americana

Role of the testicular capsule in seasonal modulation of the testis

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