Aspartame Optical Biosensor with Bienzyme-Immobilized Eggshell Membrane and Oxygen-Sensitive Optode Membrane

Abstract

An aspartame optical biosensor has been fabricated by employing a bienzyme system composed of alpha-chymotrypsin and alcohol oxidase immobilized onto an eggshell membrane and an oxygen-sensitive optode membrane as the transducer. The detection schemes involve the enzymatic reactions of aspartame leading to the depletion of the oxygen level of the medium with a concomitant enhancement of the fluorescence intensity of the oxygen-sensitive membrane. The scanning electron and transmission electron micrographs show the microstructure of the eggshell membrane which is successfully immobilized with bienzyme. Using this novel immobilization technique, the aspartame biosensor shows extremely good stability with a shelf life of at least 8 months. The rate change of the fluorescence intensity in 4 min is found to be linearly related to the concentration of aspartame. The useful analytical working range of the biosensor is from 0.056 to 3.07 mM aspartame. The effects of temperature, pH, and ionic strength on the response of the aspartame biosensor are investigated in detail. Citric acid, cyclamic acid, D-fructose, D-galactose, D-glucose, hydrogen peroxide, DL-malic acid, L-phenylalanine, saccharin, sodium benzoate, and sucrose show no interferences but ethanol interferes strongly. The aspartame biosensor has been applied to determine aspartame contents in some commercial products.

Alternative Low-cost Adsorbent for Water and Wastewater Decontamination Derived from Eggshell Waste: An Overview

“…1. Each of these membranes is composed of protein fibers that are arranged to form a semi-permeable membrane, producing an intricate lattice network of stable and water-insoluble fibers and has high surface area resulting in various applications such as adsorbent [111,[117][118][119][120][121][122] and supporting medium for enzymes immobilization [123][124][125].…”

Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

“…Moreover, the amines and amides on its surface present positively charged functional groups can be functionally modified [34]. Because of these properties, ESM has currently been used in several biomedical applications as a membrane for guided bone regeneration [35], a biological dressing to promote the infection-free healing of wounds [36] and a platform for protein immobilisation [37,38].…”

Mechanical properties of modified biofiller‐polypropylene composites

“…ES has a relatively lower density (0.4236 g/cm 3 ) compared to commercial CaCO 3 (0.4670 g/cm 3 ) [12], however the density difference of the composites will be prominent at higher filler loadings only. The generalized eggshell structure, which varies widely among species, is a protein matrix lined with mineral crystals, usually of a calcium compound such as CaCO 3 [13,14].…”