Asparagus cochinchinensis stimulates release of nerve growth factor and abrogates oxidative stress in the Tg2576 model for Alzheimer’s disease

Abstract

BackgroudUse of multifunctional drugs with neurotrophic supporting and oxidative stress suppressing activity may be considered a therapeutic strategy to protect or repair cellular damage caused during the progression of Alzheimer’s disease (AD). In this study, we investigated the therapeutic effects of aqueous extract of A. cochinchinesis root (AEAC), particularly its role as a nerve growth factor (NGF) stimulator and anti-oxidant in Tg2576 mice showing AD phenotypes of human.MethodsTg2576 mice were received 100 mg/kg/day AEAC via oral administration, while mice in the Vehicle treated group received dH2O for 4 weeks. Non-Tg littermates were used as a control group. Following AEAC treatment for 4 weeks, NGF function, anti-oxidantive status, Aβ-42 peptide level, γ-secretase expression and neuronal cell functions were analyzed in the brain of Tg2576 mice.ResultsAEAC containing flavonoids, phenols, saponins and protodioscin induced enhancement of NGF secretion and decreased intracellular ROS in the neuronal and microglial cell line. These effects as well as enhanced SOD levels were also detected in AEAC treated Tg2576 mice. The expression of p-Akt among downstream effectors of the high affinity NGF receptor was dramatically recovered in AEAC treated Tg2576 mice, while the expression of p75NTR was slightly recovered in the same group. Significant recovery on the level of Aβ-42 peptides and the expression of γ-secretase members including PS-2, APH-1 and NCT were detected in AEAC treated Tg2576 mice. Furthermore, AEAC treated Tg2576 mice showed decreased numbers of dead cells and suppressed acetyl choline esterase (AChE) activity.ConclusionsThese results suggest that AEAC contribute to improving the deposition of Aβ-42 peptides and neuronal cell injuries during the pathological progression stage of AD in the brain of Tg2576 mice through increased NGF secretion and suppressed oxidative stress.

Treadmill Exercise Decreases Aβ Deposition and Counteracts Cognitive Decline in APP/PS1 Mice, Possibly via Hippocampal Microglia Modifications

“…Oxidative stress leads to oxidative damage of many cellular components in AD (Lee et al, 2018). Therefore, we examined the effects of treadmill exercise on oxidative damage in the hippocampi of APP/PS1 mice using MDA, SOD, and Mn-SOD.…”

Anti-Oxidant Activity of Gallotannin-Enriched Extract of Galla Rhois Can Associate with the Protection of the Cognitive Impairment through the Regulation of BDNF Signaling Pathway and Neuronal Cell Function in the Scopolamine-Treated ICR Mice

“…The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity level was determined using a previously described method [21,22]. Briefly, the powdered GEGR was dissolved in 50% EtOH (100 μL) to obtain 12 different GEGR concentrations (1 to 2000 μg/mL), which were then mixed with 100 μL of 0.1 mM DPPH (Sigma-Aldrich Co., St. Louis, MO, USA) in a 95% ethanol solution or with 100 μL of 95% ethanol solution (control).…”

Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice

“…Intracellular ROS levels were measured in NHDF cells by staining with 2 ,7 -dichlorofluorescein diacetate (DCF-DA) (Sigma-Aldrich Co.) as previously described [41]. Briefly, NHDF cells seeded at 4.5 × 10 5 cells/3 mL in 6-well plates were cultured to 70-80% confluent, irradiated at 50 mJ, and treated with CPO or GEGR and 25 µM DCFH-DA and incubated for 30 min at 37 • C. After washing twice with 1× PBS, the degree of green fluorescence was observed in DCF-DA stained cell under a fluorescence microscope (200×; Eclipse TX100, Nikon, Tokyo, Japan).…”