Asip and MCIR cDNA polymorphism in alpaca

Chandramohan, Bathrachalam; Manna, Vincenzo; Renieri, Carlo; Terza, Antonietta La

doi:10.3920/978-90-8686-727-1_11

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Mutations in this gene have shown to cause the black coat color phenotype in different species, such as guinea pigs (Lai et al, 2019), black-bone chicken (Yu et al, 2019), sheep (Norris and Whan, 2008; Royo et al, 2008), Iranian Markhoz goats (Nazari-Ghadikolaei et al, 2018), donkeys (Abitbol et al, 2015), horses (Rieder et al, 2001), dogs (Kerns et al, 2004), cats (Eizirik et al, 2003), and impala antelope (Miller et al, 2016). In camelids the agouti signaling protein gene ( ASIP ) is involved in fiber color development in alpacas (Bathrachalam et al, 2011; Chandramohan et al, 2013), llamas (Daverio et al, 2016) and dromedaries (Almathen et al, 2018; Alshanbari et al, 2019). Sequence variants (SNPs) in other genes from this linkage group have been associated with color phenotypes in several mammalian species.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal Localization of Candidate Genes for Fiber Growth and Color in Alpaca (Vicugna pacos)

et al. 2019

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The alpaca ( Vicugna pacos ) is an economically important and cultural signature species in Peru. Thus, molecular genomic information about the genes underlying the traits of interest, such as fiber properties and color, is critical for improved breeding and management schemes. Current knowledge about the alpaca genome, particularly the chromosomal location of such genes of interest is limited and lags far behind other livestock species. The main objective of this work was to localize alpaca candidate genes for fiber growth and color using fluorescence in situ hybridization (FISH). We report the mapping of candidate genes for fiber growth COL1A1 , CTNNB1 , DAB2IP , KRT15 , KRTAP13-1 , and TNFSF12 to chromosomes 16, 17, 4, 16, 1, and 16, respectively. Likewise, we report the mapping of candidate genes for fiber color ALX3 , NCOA6 , SOX9 , ZIC1 , and ZIC5 to chromosomes 9, 19, 16, 1, and 14, respectively. In addition, since KRT15 clusters with five other keratin genes ( KRT31 , KRT13 , KRT9 , KRT14 , and KRT16 ) in scaffold 450 (Vic.Pac 2.0.2), the entire gene cluster was assigned to chromosome 16. Similarly, mapping NCOA6 to chromosome 19, anchored scaffold 34 with 8 genes, viz., RALY , EIF2S2 , XPOTP1 , ASIP , AHCY , ITCH , PIGU , and GGT7 to chromosome 19. These results are concordant with known conserved synteny blocks between camelids and humans, cattle and pigs.

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Section: Discussionmentioning

confidence: 99%

Chromosomal Localization of Candidate Genes for Fiber Growth and Color in Alpaca (Vicugna pacos)

et al. 2019

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“…Copy number variation of ASIP has also been associated with light and dark coats in goats and sheep ( Norris and Whan, 2008 ; Fontanesi et al, 2011 ; Dong et al, 2015 ). In alpaca and llama, several polymorphic sites have been identified on MC1R and ASIP ( Powell et al, 2008 ; Bathrachalam et al, 2011 ; Feeley et al, 2011 ; Daveiro et al, 2016 ). However, these substitutions have yet to be evaluated in their wild counterparts.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation in Coat Colour Genes MC1R and ASIP Provides Insights Into Domestication and Management of South American Camelids

et al. 2018

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The domestication of wild vicuña and guanaco by early pre-Inca cultures is an iconic example of wildlife management and domestication in the Americas. Although domestic llamas and alpacas were clearly selected for key, yet distinct, phenotypic traits, the relative patterns and direction of selection and domestication have not been confirmed using genetic approaches. However, the detailed archaeological records from the region suggest that domestication was a process carried out under significant control and planning, which would have facilitated coordinated and thus extremely effective selective pressure to achieve and maintain desired phenotypic traits. Here we link patterns of sequence variation in two well-characterised genes coding for colour variation in vertebrates and interpret the results in the context of domestication in guanacos and vicuñas. We hypothesise that colour variation in wild populations of guanacos and vicunas were strongly selected against. In contrast, variation in coat colour variation in alpaca was strongly selected for and became rapidly fixed in alpacas. In contrast, coat colour variants in llamas were of less economic value, and thus were under less selective pressure. We report for the first time the full sequence of MC1R and 3 exons of ASIP in 171 wild specimens from throughout their distribution and which represented a range of commonly observed colour patterns. We found a significant difference in the number of non-synonymous substitutions, but not synonymous substitutions among wild and domestics species. The genetic variation in MC1R and ASIP did not differentiate alpaca from llama due to the high degree of reciprocal introgression, but the combination of 11 substitutions are sufficient to distinguish domestic from wild animals. Although there is gene flow among domestic and wild species, most of the non-synonymous variation in MC1R and ASIP was not observed in wild species, presumably because these substitutions and the associated colour phenotypes are not effectively transmitted back into wild populations. Therefore, this set of substitutions unequivocally differentiates wild from domestic animals, which will have important practical application in forensic cases involving the poaching of wild vicuñas and guanacos. These markers will also assist in identifying and studying archaeological remains pre- and post-domestication.

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“…Candidate polymorphisms at MC1R and ASIP genes which may impair the structure and functionality of both proteins have been already shown to affect coat colour in this species (Powell et al 2008;Feeley and Munyard 2009;Bathrachalam et al 2011;Feeley et al 2011;Guridi et al 2011;Chandramohan et al 2013Chandramohan et al , 2015. In fact, the Agouti locus in white and coloured (black and brown) alpacas has been found to be affected by three different mutations consisting in two independent SNPs and one deletion of 57 bp (Feeley et al 2011;Chandramohan et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Interaction between the melanocortin 1 receptor (MC1R) and agouti signalling protein genes (ASIP), and their association with black and brown coat colour phenotypes in peruvian alpaca

Pallotti

Chandramohan

Pediconi

et al. 2020

Italian Journal of Animal Science

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Asip and MCIR cDNA polymorphism in alpaca

Cited by 3 publications

References 6 publications

Chromosomal Localization of Candidate Genes for Fiber Growth and Color in Alpaca (Vicugna pacos)

Chromosomal Localization of Candidate Genes for Fiber Growth and Color in Alpaca (Vicugna pacos)

Genetic Variation in Coat Colour Genes MC1R and ASIP Provides Insights Into Domestication and Management of South American Camelids

Interaction between the melanocortin 1 receptor (MC1R) and agouti signalling protein genes (ASIP), and their association with black and brown coat colour phenotypes in peruvian alpaca

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