Asiatic Acid Protects Dopaminergic Neurons from Neuroinflammation by Suppressing Mitochondrial ROS Production

Chen, Dong; Zhang, Xiaoya; Sun, Jing; Cong, Qijie; Chen, Weixiong; Ahsan, Hafiz Muhammad; Gao, Jing; Qian, Jinjun

doi:10.4062/biomolther.2018.188

Cited by 44 publications

(23 citation statements)

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“…The acidic forms, which occur in the extract at concentrations six-to-seven fold lower than their corresponding glycosides, may be formed by the glycosidases of the microbiota, thus explaining their higher plasmatic concentrations. C. asiatica L. terpenes in rat plasma occur at nanomolar concentrations; however, it has recently been demonstrated that those concentrations of asiatic acid may protect dopaminergic neurons from inflammation by reducing reactive oxygen species (ROS) production [27]. Indeed, a synergic effect among terpenes, which may increase their biological activity in vivo, cannot be excluded.…”

Section: Discussionmentioning

confidence: 99%

“…Blood was collected from the heart for the quantification of the components of the extract in the plasma. After decapitation, the prefrontal cortex, defined as cingulate cortex (Cg) 1-3 and infralimbic sub-regions (plates 6-10), was immediately dissected from 2 mm thick slices, whereas the dorsal and ventral hippocampi (plates [25][26][27][28][29][30][31][32][33] were dissected from the whole brain according to the atlas of Paxinos and Watson [15] and then stored at −80 • C for the subsequent molecular analyses.…”

Section: Treatment and Behavioral Testmentioning

confidence: 99%

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Centella asiatica L. Phytosome Improves Cognitive Performance by Promoting Bdnf Expression in Rat Prefrontal Cortex

et al. 2020

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A wide range of people in the world use natural remedies as primary approaches against illnesses. Accordingly, understanding the mechanisms of action of phytochemicals has become of great interest. In this context, Centella asiatica L. is extensively used, not only as anti-inflammatory or antioxidant agent but also as brain tonic. On this basis, the purpose of this study was to evaluate whether the chronic administration of C. asiatica L. to adult male rats was able to improve the expression of Bdnf, one of the main mediators of brain plasticity. Moreover, we assessed whether the treatment could affect the cognitive performance in the novel object recognition (NOR) test. We confirmed the presence of the main compounds in the plasma. Furthermore, C. asiatica L. administration induced an increase of Bdnf in the prefrontal cortex, and the administration of the higher dose of the extract was able to improve cognitive performance. Finally, the increase in the preference index in the NOR test was paralleled by a further increase in Bdnf expression. Overall, we highlight the ability of C. asiatica L. to affect brain functions by increasing Bdnf expression and by enhancing the cognitive performance.

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Section: Discussionmentioning

confidence: 99%

Section: Treatment and Behavioral Testmentioning

confidence: 99%

Centella asiatica L. Phytosome Improves Cognitive Performance by Promoting Bdnf Expression in Rat Prefrontal Cortex

et al. 2020

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“…Our results demonstrate that AA supplementation clearly ameliorated oxidative stress because it not only decreased intracellular ROS levels but also maintained intracellular GSH levels during the process of IVA. Similarly, AA was reported to reduce intracellular ROS levels in microglial cells [ 52 ], HepG2 cells [ 53 ], and SH-SYS5Y cells [ 54 ] and increase superoxide dismutase activity and intracellular GSH production in spinal cord injury in rats [ 55 ]. Therefore, we hypothesized that AA might reduce oxidative stress by removing intracellular ROS and increasing GSH synthesis, thus delaying the decline in porcine oocyte quality during the process of IVA.…”

Section: Discussionmentioning

confidence: 99%

Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs

Li²,

Diao³

et al. 2020

Aging

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As a pentacyclic triterpene in Centella asiatica , asiatic acid (AA) is a powerful antioxidant with many bioactivities. In the present research, we investigated whether AA has the potential to rescue the decrease in porcine oocyte quality that occurs during in vitro aging (IVA). Mature porcine oocytes were collected and then continuously cultured for an additional 24 h or 48 h with or without AA in maturation medium as an IVA model. The results revealed that AA supplementation reduced the percentage of abnormal aged porcine oocytes during IVA. Furthermore, AA supplementation effectively maintained aged porcine oocyte developmental competence, both parthenogenetic activation and in vitro fertilization. The number of sperm that bound to the zona pellucida on aged porcine oocytes was higher in the AA-supplemented group than in the non-supplemented group. Moreover, AA supplementation not only blocked IVA-induced oxidative stress but also maintained intracellular GSH levels and reduced the percentage of early apoptosis aged porcine oocytes. Mitochondrial functions were disordered during the IVA process. The intracellular ATP levels and mitochondrial membrane potential in aged porcine oocytes were dramatically increased by AA supplementation. Therefore, AA has beneficial effects on porcine oocyte quality and developmental potential maintenance during IVA.

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“…SOD and GSH are the major antioxidant enzymes for scavenging free radicals, and most hepatotoxic drugs impair their functions [35]. GSH is considered to be a key antioxidant involved in mitochondrial dysfunction and apoptosis, and a GSH imbalance may lead to redox imbalance [36,37]. MDA is produced by free-radical-mediated lipid peroxidation and is used as a marker of oxidative stress [38,39].…”

Section: Discussionmentioning

confidence: 99%

Screening of the Hepatotoxic Components in Fructus Gardeniae and Their Effects on Rat Liver BRL-3A Cells

Lan

et al. 2019

Molecules

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Fructus Gardeniae (FG) is a common Chinese medicine and food. However, the toxicity of FG has drawn increasing concern, especially its hepatotoxicity. The purpose of this study was to screen the hepatotoxic components of FG and evaluate their effects on rat liver BRL-3A cells. The chemical composition of FG was determined by HPLC-ESI-MS. CCK-8 assay was used to evaluate the cytotoxicity of ten chemical components from FG, and then the toxic components with significant inhibitory activity were selected for further study. The results showed that geniposide, genipin, genipin-1-gentiobioside, gardenoside, and shanzhiside all suppress cells viability. Apoptosis assays further indicated that geniposide and its metabolite genipin are the main hepatotoxic components of FG. Pretreatment of cells with geniposide or genipin increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The activities of superoxide dismutase (SOD) and glutathione (GSH) were decreased, while the malondialdehyde (MDA) level was increased. The cell contents of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and nitric oxide (NO) were also increased. Molecular docking simulations were used to investigate the mechanism of FG-induced hepatotoxicity, revealing that geniposide and genipin bind strongly to the pro-inflammatory factor TNFR1 receptor of the NF-κB and MAPK signaling pathways. The obtained results strongly indicate that the hepatotoxicity of FG is caused by iridoids compounds. Genipin had the most significant hepatotoxic effect. These toxic substances destroy the cell antioxidant defense system, increasing inflammatory injury to the liver cells and leading to apoptosis and even necrosis. Thus, this study lays a foundation for toxicology research into FG and its rational application.

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Asiatic Acid Protects Dopaminergic Neurons from Neuroinflammation by Suppressing Mitochondrial ROS Production

Cited by 44 publications

References 44 publications

Centella asiatica L. Phytosome Improves Cognitive Performance by Promoting Bdnf Expression in Rat Prefrontal Cortex

Centella asiatica L. Phytosome Improves Cognitive Performance by Promoting Bdnf Expression in Rat Prefrontal Cortex

Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs

Screening of the Hepatotoxic Components in Fructus Gardeniae and Their Effects on Rat Liver BRL-3A Cells

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