Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs

Hu, Wei; Li, Xiao Xia; Diao, Yun Fei; Qi, Jiajia; Wang, Dali; Zhang, Jiabao; Sun, Boxing; Liang, Shuang

doi:10.18632/aging.202184

Cited by 6 publications

(3 citation statements)

References 73 publications

(20 reference statements)

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“…In addition to heart, kidney and liver are negatively affected following DOX administration due to an increase in oxidative stress and inflammation [92,93]. Asiatic acid (AA) is another phytochemical that has been under attention due to its efficacy in preventing ageing, improving wound healing and exerting anti-tumor activity [94][95][96][97]. Recently, it has been shown that AA possesses high antioxidant potential that is of importance in reducing toxic effects of DOX against major organs of body.…”

Section: Natural Compounds In Ameliorating Doxorubicin-mediated Toxicitymentioning

confidence: 99%

Nrf2 Signaling Pathway in Chemoprotection and Doxorubicin Resistance: Potential Application in Drug Discovery

et al. 2021

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Doxorubicin (DOX) is extensively applied in cancer therapy due to its efficacy in suppressing cancer progression and inducing apoptosis. After its discovery, this chemotherapeutic agent has been frequently used for cancer therapy, leading to chemoresistance. Due to dose-dependent toxicity, high concentrations of DOX cannot be administered to cancer patients. Therefore, experiments have been directed towards revealing underlying mechanisms responsible for DOX resistance and ameliorating its adverse effects. Nuclear factor erythroid 2-related factor 2 (Nrf2) signaling is activated to increase levels of reactive oxygen species (ROS) in cells to protect them against oxidative stress. It has been reported that Nrf2 activation is associated with drug resistance. In cells exposed to DOX, stimulation of Nrf2 signaling protects cells against cell death. Various upstream mediators regulate Nrf2 in DOX resistance. Strategies, both pharmacological and genetic interventions, have been applied for reversing DOX resistance. However, Nrf2 induction is of importance for alleviating side effects of DOX. Pharmacological agents with naturally occurring compounds as the most common have been used for inducing Nrf2 signaling in DOX amelioration. Furthermore, signaling networks in which Nrf2 is a key player for protection against DOX adverse effects have been revealed and are discussed in the current review.

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Section: Natural Compounds In Ameliorating Doxorubicin-mediated Toxicitymentioning

confidence: 99%

Nrf2 Signaling Pathway in Chemoprotection and Doxorubicin Resistance: Potential Application in Drug Discovery

et al. 2021

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“…Our results showed that FA alleviated the decrease in MMP and partially restored mitochondrial function in aged oocytes. These results provide evidence that exogenous antioxidant supplementation can improve mitochondrial function in aged oocytes and thus promote oocyte developmental potential [ 42 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 92%

Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis

Yin,

Zhang,

Wang

et al. 2023

Aging

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Ferulic acid (FA) is a well-known natural antioxidant that scavenges oxygen free radicals and alleviates oxidative stress. This study investigated the chemopreventive potential of FA against bovine oocyte quality decline during in vitro aging. The results showed that 5 μM FA supplementation decreased the abnormality rate of in vitro -aged bovine oocytes. In addition, FA supplementation effectively improved antioxidant capacity by removing excessive ROS and maintaining intracellular GSH levels and antioxidant enzyme activity. The mitochondrial activity, mitochondrial membrane potential and intracellular ATP levels in aged bovine oocytes were obviously enhanced by FA supplementation. Furthermore, FA supplementation reduced in vitro aging-induced DNA damage and maintained DNA stability in bovine oocytes. Moreover, sperm binding assay showed the number of sperm that bound to the zona pellucida on aged bovine oocytes was significantly higher in the FA supplemented group than in the Aged group. Therefore, FA is beneficial for maintaining in vitro -aged bovine oocyte quality and could become a potential antioxidant for preventing bovine oocyte in vitro aging during in vitro maturation.

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“…Mitochondria are a site of energy metabolism and are involved in cell apoptosis and death. Several studies have shown that mitochondrial function influences oocyte developmental potential and is associated with subsequent embryonic development, such as that of PA, IVF, and SCNT embryos (Liang et al, 2018;An et al, 2019;Hu et al, 2020;Nie et al, 2020). Recent research has suggested that ferroptosis can lead to mitochondrial dysfunction, including loss of the MitoMP, enhanced mitochondrial fragmentation, and reduced mitochondrial respiration, in neuronal HT22 cells and mouse embryonic fibroblasts (Jelinek et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Iron Overload-Induced Ferroptosis Impairs Porcine Oocyte Maturation and Subsequent Embryonic Developmental Competence in vitro

Zhang

Wang

et al. 2021

Front. Cell Dev. Biol.

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Accumulating evidence indicates that ferroptosis is an iron-dependent form of regulated cell death. This type of iron-dependent programmed cell death is different from traditional forms of regulated cell death, such as apoptosis and autophagy. However, the role of ferroptosis in porcine oocyte maturation and the associated mechanism remain unclear. In the present research, we investigated the effects of ferric ammonium citrate (FAC), a specific ferroptosis inducer, on porcine oocyte meiotic maturation and quality and subsequent embryonic developmental competence. FAC treatment caused obvious accumulation of intracellular ferrous ions in porcine oocytes. At the end of the in vitro maturation (IVM) period, there was a significant decrease in the polar body (PB) extrusion rate and an increase in the percentage of abnormal oocytes in the FAC treatment groups, indicating that iron overload-induced ferroptosis may suppress the meiotic process during porcine oocyte maturation. We also found that after FAC treatment, the subsequent two-cell rate, four-cell rate and blastocyst formation rate were significantly decreased in porcine parthenogenetic activation (PA) embryos, indicating that iron overload-induced ferroptosis decreased porcine oocyte quality. Further analysis revealed that FAC treatment not only enhanced intracellular reactive oxygen species (ROS) generation, decreased intracellular free thiol levels and induced mitochondrial dysfunction but also triggered autophagy in porcine oocytes. Taken together, these findings suggest that iron overload-induced ferroptosis impairs porcine oocyte meiosis and decreases porcine oocyte quality, possibly by increasing oxidative stress, inducing mitochondrial dysfunction and triggering autophagy.

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Asiatic acid protects oocytes against in vitro aging-induced deterioration and improves subsequent embryonic development in pigs

Cited by 6 publications

References 73 publications

Nrf2 Signaling Pathway in Chemoprotection and Doxorubicin Resistance: Potential Application in Drug Discovery

Nrf2 Signaling Pathway in Chemoprotection and Doxorubicin Resistance: Potential Application in Drug Discovery

Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis

Iron Overload-Induced Ferroptosis Impairs Porcine Oocyte Maturation and Subsequent Embryonic Developmental Competence in vitro

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