Asiatic Acid Fabricated Nanoconstructs to Mitigate Amyloid Beta1-42 Induced Injury in SH-SY5Y Cells In-Vitro and Ameliorates Cognitive Impairment by Dual Cholinesterase Inhibition and Attenuation of Oxidative Stress In-Vivo

Halder, Tripti; Patel, Bharat; Acharya, Niyati

doi:10.1007/s11095-022-03428-2

Cited by 3 publications

(4 citation statements)

References 43 publications

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“…Rats were intraperitoneal (i.p.) injected with CA‐AA‐NLCs and SA‐CA‐AA‐NLCs at a concentration of 75 mg/kg body weight ( n = 3) and plasma and brain kinetic profile were determined according our previous article (Halder et al, 2022b).…”

Section: Methodsmentioning

confidence: 99%

“…In vitro cytotoxicity studies in SH-SY5Y and hCMEC/D3 cells In vitro, cytotoxicity and neuroprotective studies of NLCs were evaluated in SH-SY5Y and hCMEC/D3 cells as per our previous paper (Halder et al, 2022b).…”

Section: Cell Line Studiesmentioning

confidence: 99%

“…But the therapeutic potency of AA is obstructed due to poor solubility, rapid metabolism and short biological half-life leading to poor bioavailability into the brain. In our previous study, we showed, AA loaded NLCs provided better bioavailability of AA in brain cells (Halder et al, 2022b). Cationic AA functionalized NLC (CA-AA-NLCs) and SA surface modified CA-AA-NLCs (SA-CA-AA-NLCs) were employed and evaluated for their treatment potential in AD.…”

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confidence: 99%

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Development and evaluation of multi‐functionalized sialic acid conjugated asiatic acid nanoconstruct to mitigate cognitive deficits in Alzheimer's disease

Halder,

Saha,

Dhas

et al. 2024

Drug Development Research

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Sialic acid (SA) serves a critical role in neuronal repair and cognitive functions. SA is a nine‐carbon carboxylated sugar with a glycoconjugate cap that acts as a ligand and surface decoration with SA facilitates delivery to the target site. The present research aimed to develop SA surface modified AA nanostructured lipid carrier (NLCs) with carbodiimide conjugation method. Sterylamine, poloxamer 188 and tween 80 were used as surfactants and several characterization studies including, differential scanning calorimetry, fourier transform infrared spectroscopy and x‐ray photon spectroscopy were analyzed. Further, in vitro, neuroprotective efficiency was evaluated in SH‐SY5Y cells and hCMEC/D3 cells and found significant potential effects with the treatments of developed NLCs. Pharmacodynamics studies were also assessed in beta‐amyloid‐injected rats following quantification of Alzheimer's disease (AD) hallmarks like, Aβ(1–42), tau‐protein, glycogen synthase kinase‐3β levels, interleukin‐6 and tumor necrosis factor‐α for neuroinflammatory responses. Characterization studies revealed the conjugation on developed NLCs. The in vitro and in vivo results showed significant effects of SA decorated NLCs in reversing the damage by toxicant which was further characterized by the levels of neurotransmitters like acetylcholinesterase, butyrylcholinesterase. The results revealed significant (p < .05) refurbishment of cholinergic functions after 28 days of treatment of developed NLCs. These preclinical findings support the use of SA as a ligand to deliver the AA at targeted site as well as to mitigate the cognitive deficits in AD.

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Section: Methodsmentioning

confidence: 99%

Section: Cell Line Studiesmentioning

confidence: 99%

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confidence: 99%

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Development and evaluation of multi‐functionalized sialic acid conjugated asiatic acid nanoconstruct to mitigate cognitive deficits in Alzheimer's disease

Halder,

Saha,

Dhas

et al. 2024

Drug Development Research

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“…These include solid lipid nanoparticles poly D, L-lactic-co-glycolic acid (PLGA) based NLCsand surface conjugation of albumin nanoparticles with glutathione (161) . The NMDA and AMPA receptors located in the BBB act as endogenous ligands for glutathione, it promotes asiatic acid permeability in the brain and increases the passage of asiatic acid to the brain (162) . Tripty Halder et aldiscovered that AA loaded nanostructured lipid carriers (AAN) protect amyloid Beta 1-42 induced cognitive impairment and oxidative stress in SHSY5Y Cells (163) .…”

Section: Permeability Of Blood Brain Barrier and Asiatic Acidmentioning

confidence: 99%

Preventive Mechanism, Therapeutic Property,Pharmacokinetics And Benefitsof Asiatic Acid - A Triterpenoid Of Centella Asiatica In Alzheimer Disease: An In-Depth Review

Chakraborty,

Sinha

2023

Asian J Pharm Res Dev

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Alzheimer's disease (AD) remains the most prevalent form of age-related dementia worldwide, and it has no cure. Memory loss, difficulty communicating, depression, agitation, mood swings, and psychosis all develop gradually in this disorder. Reduced physical activity, infection, smoking, and the prevalence of diseases like obesity and diabetes all pose a risk for the development of AD. Current synthetic medications only alleviate symptoms by targeting a single molecule, so they can't deal with the complex pathogenesis of AD. The scientific community is actively working to characterise therapeutic agents derived from plants that have shown promise in the literature in treating AD due to their perceived efficacy, safety, and availability. Traditional Chinese medicine includes the use of the plant Centella asiatica for its purported benefits to cognitive performance and memory. Pentacyclic triterpenes are largely responsible for its therapeutic and medicinal effects, which include accelerated enhanced memory. These pentacyclic triterpenoids are asiaticoside and madecassoside as saponins and their aglycones, asiatic acids and madecassic acids. Amongst other triterpenoid Asiatic acid has several therapeutic properties against AD like anti-cholinesterase, neuroprotective and anti-inflammatory. This review focused on the pathophysiology of AD followed by a detailed account on Asiatic acid and the research findings to date related to its mechanism of action on AD, advantage over other two terpenoid, BBB permeability, Pharmacokinetic, molecular docking and different therapeutic activities.

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Targeted Nano-based Drug Delivery in Alzheimer's Disease and Dementia

Payel Mal,

Rath,

Halder

2024

Smart Nanomaterials Technology

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Asiatic Acid Fabricated Nanoconstructs to Mitigate Amyloid Beta1-42 Induced Injury in SH-SY5Y Cells In-Vitro and Ameliorates Cognitive Impairment by Dual Cholinesterase Inhibition and Attenuation of Oxidative Stress In-Vivo

Cited by 3 publications

References 43 publications

Development and evaluation of multi‐functionalized sialic acid conjugated asiatic acid nanoconstruct to mitigate cognitive deficits in Alzheimer's disease

Development and evaluation of multi‐functionalized sialic acid conjugated asiatic acid nanoconstruct to mitigate cognitive deficits in Alzheimer's disease

Preventive Mechanism, Therapeutic Property,Pharmacokinetics And Benefitsof Asiatic Acid - A Triterpenoid Of Centella Asiatica In Alzheimer Disease: An In-Depth Review

Targeted Nano-based Drug Delivery in Alzheimer's Disease and Dementia

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