Ascorbyl palmitate-loaded chitosan nanoparticles: Characteristic and polyphenol oxidase inhibitory activity

Kim, Mi Kyung; Lee, Ji-Soo; Kim, Kwang Yup; Lee, Hyeon Gyu

doi:10.1016/j.colsurfb.2012.09.038

Cited by 22 publications

(15 citation statements)

References 23 publications

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“…1) were collected. These fractions (peaks [10][11][12][13][14][15][16][17][18][19][20] corresponded to NaCl concentrations between 0.1-0.3 M. The protein content of the elution was determined using Coomassie Brilliant Blue and measured at 595 nm. Enzyme activity was determined using catechol and measured at 420 nm (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…[12,13] Heat treatment and sulfites inhibit enzyme browning; ascorbic acid, cysteine, and glutathione have also been reported to prevent browning in many articles. [14,15] Some reducing agents are oxidized, such as cysteine, β-mercaptoethanol and ascorbic acid. Other reducing agents are phenol scavengers, such as PVPP (polyvinyl polypyrrolidone) and PEG (polyethylene glycol); these compounds are used in PPO homogenate buffer to prevent to o-quinone formation.…”

Section: Introductionmentioning

confidence: 99%

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Purification and Characterization of Polyphenol Oxidase from Hemşin Apple (Malus communisL.)

Aydin

Gülçın

Alwasel

2015

International Journal of Food Properties

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The polyphenol oxidase (PPO) enzyme was purified and characterized from Hemşin Apple (Malus communis L.), which was organically grown in Hemşin, in the Rize province of Turkey. Enzyme (PPO) activation was determined with catechol substrate. Apples were homogenized with homogenate buffer (pH 8.5). This process was followed by precipitation with (20-80%) saturated solid (NH 4 ) 2 SO 4 and dialysis. Finally, purification with DE52-Cellulose ion-exchange and Sephadex G-25 columns was performed. Experiments were performed at an optimum pH (5.5) and optimum temperature (30-40°C). The kinetic and thermal parameters Km (3.40 mM), Vmax (333.3 EU/mL.min), Ea (3.57 kcal), ΔH (2.968 kcal/mol), Q 10 (1.33), k cat (24.57 min −1 ) and V 0 (7.2x10 3 mM −1 .min −1 ) were assessed. Additionally, the effects of Mg 2+ , Pb 2+ , Fe 2+ , Fe 3+ , Cd 2+ , Cu 2+ , Zn 2+ , Co 2+ , Al 3+ , Mn 2+ and Na + on enzyme activity was recorded, and the IC 50 values, K İ values and inhibition types were determined.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of Polyphenol Oxidase from Hemşin Apple (Malus communisL.)

Aydin

Gülçın

Alwasel

2015

International Journal of Food Properties

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“…PA is almost insoluble in water at room temperature, and liposomes are a good choice for PA to deliver into tumor2425. Moreover, the chemical stability of PA was reported to be enhanced when it was encapsulated in solid lipid nanoparticles2627. The aim of present study was to co-deliver PA and docetaxel (DOC) via a liposome-encapsulated nanoparticles system and evaluate its anticancer effect in MCF-7, HepG-2, PC-3 cell lines.…”

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confidence: 99%

Co-delivery of docetaxel and palmitoyl ascorbate by liposome for enhanced synergistic antitumor efficacy

Li¹,

Guo²,

Feng³

et al. 2016

Sci Rep

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Palmitoyl ascorbate (PA) as an antioxidant has the potential for the treatment of cancer. In the present study, a nanocarrier system was developed for co-delivery of docetaxel (DOC) with palmitoyl ascorbate and the therapeutic efficacy of a combination drug regimen was investigated. For this purpose, different ratios of docetaxel and palmitoyl ascorbate were co-encapsulated in a liposome and they all showed high encapsulation efficiency. The average diameters of the liposomes ranged from 140 to 170 nm. Negative zeta potential values were observed for all systems, ranged from −40 mV to −56 mV. Studies on drug release and cellular uptake of the co-delivery system demonstrated that both drugs were effectively taken up by the cells and released slowly. Moreover, the liposome loading drugs with DOC/PA concentration ratio of 1:200 showed the highest anti-tumor activity to three different types of tumor cells. The higher in vivo therapeutic efficacy with lower systemic toxicity of the DOC-PA200-LPs was also verified by the H22 tumor bearing mice model. Our results showed that such co-loaded delivery systems could serve as a promising therapeutic approach to improve clinical outcomes against hepatic carcinoma.

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“…Banana fruit undergoes rapid enzymatic browning as a result of tissue disruption and exposure to oxygen during peeling and slicing operations prior to further processing, due to the mediation of PPO (Kim et al 2013). Sulfites have long been used as a low cost additive in food products for their antbrowning ability (Mogol et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparison of anti-browning ability and characteristics of the fractionated Maillard reaction products with different polarities

Yuan

Wang

et al. 2015

J Food Sci Technol

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The Maillard reaction products (MRPs) prepared from aqueous equimolar glucose with 1 M cysteine model solution when heated at 110°C for 1 h was previously recognized as strong browning inhibitors for banana fruit. This study investigated further the antibrowning ability of the fractionated fractions with different polarity using solvent extraction following a ultraf i l t r a t i o n w i t h 3 . 5 k u MW C O m em b ra ne an d characteristized antioxidant activity including DPPH• scavenging ability and reducing power, chelating copper activity, inhibitory ability and the inhibitory constants of banana PPO. Extracts with different polarity were obtained by methanol, ethanol, n-butanol and isopropanol, which were designated as MRP (MeOH) ,MRP (EtOH) ,MRP (nBuOH) and MRP (IPA) , respectively. Application of the Lineweaver-Burk plotting showed that all MRPs were mixed-type 1 inhibitors whose K is was larger than corresponding K i , with K i values ranging between 0.297 and 0.035 mg/mL MRPs. MRP (EtOH) showed the strongest anti-browning ability from the visual observation, further evidenced by its lowest K i and IC 50 in terms of the inhibition of PPO activity and DPPH• scavenging ability, with a different anti-browning mechanism vs. Na 2 S 2 O 5 . The strong copper chelating ability of the MRPs may account for the antioxidant ability and PPO activity inhibition to an extent. The effective anti-browning components from MRPs in glucose-cysteine model could be enriched by the combination of ultrafitration with ethanol extraction. And the extract may be applicable in food system.

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Ascorbyl palmitate-loaded chitosan nanoparticles: Characteristic and polyphenol oxidase inhibitory activity

Cited by 22 publications

References 23 publications

Purification and Characterization of Polyphenol Oxidase from Hemşin Apple (Malus communisL.)

Purification and Characterization of Polyphenol Oxidase from Hemşin Apple (Malus communisL.)

Co-delivery of docetaxel and palmitoyl ascorbate by liposome for enhanced synergistic antitumor efficacy

Comparison of anti-browning ability and characteristics of the fractionated Maillard reaction products with different polarities

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