Ascorbic acid-loaded gliadin nanoparticles as a novel nutraceutical formulation

Voci, Silvia; Gagliardi, Agnese; Fresta, Massimo; Cosco, Donato

doi:10.1016/j.foodres.2022.111869

Cited by 16 publications

(14 citation statements)

References 93 publications

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“…Photon Correlation Spectroscopy (PCS) was used to evaluate the mean diameter, size distribution and zeta potential of the systems using a 1:50 dilution and applying the third-order cumulant fitting correlation function (Zetasizer Nano ZS apparatus, Malvern Panalytical Ltd., Spectris plc, Malvern, UK) [ 37 ]. Transmission Electron Microscopy (TEM) was used in order to investigate the morphology of the nanosystems, as previously described [ 35 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, a Turbiscan Lab Expert ® apparatus (Formulaction, Toulouse, France) was employed with the aim of evaluating the stability of the various samples; the results were processed using Turby Soft 2.0 software (Formulaction, Toulouse, France) and expressed as Turbiscan Stability Index (TSI) as a function of time and temperature [ 35 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…Each sample was centrifuged as described in Section 2.2 , and the supernatant analyzed at a λ max of 480 nm. The EE% was calculated as follows: EE (%) = D e /D t × 100 where D e and D t represent the amount of compound that became entrapped within the polymeric matrix and the amount of DOX initially added, respectively [ 35 ]. No interference between DOX and the other components of the GNPs was observed.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the iodine-iodide assay was applied in order to quantify the amount of Brij effectively integrated into the colloidal systems [ 23 , 35 ]. The drug loading capacity (LC) of the various formulations was calculated as the ratio percentage between the amount of entrapped DOX versus the total weight of the nanoparticles, according to the following equation: LC (%) = (Entrapped DOX)/(Total weight of nanoparticles) × 100 …”

Section: Methodsmentioning

confidence: 99%

“…For these reasons, the current study was designed to investigate the suitability of gliadin nanoparticles (GNPs) in antitumor therapy, and to this end DOX was used as a model of a water-soluble drug. Notwithstanding the inherent hydrophobic character of the polymer, our research group has recently demonstrated that polyoxyethylene (2) oleyl ether -stabilized gliadin nanoparticles can efficiently retain hydrophilic compounds [ 34 , 35 ]. The idea was to evaluate the physico-chemical and technological features of the DOX-loaded nanosystems in order to identify the best formulation to be used for in vitro experiments.…”

Section: Introductionmentioning

confidence: 99%

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Gliadin Nanoparticles Containing Doxorubicin Hydrochloride: Characterization and Cytotoxicity

et al. 2023

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Doxorubicin hydrochloride (DOX) is a well-known antitumor drug used as first line treatment for many types of malignancies. Despite its clinical relevance, the administration of the compound is negatively affected by dose-dependent off-target toxicity phenomena. Nanotechnology has helped to overcome these important limitations by improving the therapeutic index of the bioactive and promoting the translation of novel nanomedicines into clinical practice. Herein, nanoparticles made up of wheat gliadin and stabilized by polyoxyethylene (2) oleyl ether were investigated for the first time as carriers of DOX. The encapsulation of the compound did not significantly affect the physico-chemical features of the gliadin nanoparticles (GNPs), which evidenced a mean diameter of ~180 nm, a polydispersity index < 0.2 and a negative surface charge. The nanosystems demonstrated great stability regarding temperature (25–50 °C) and were able to retain high amounts of drug, allowing its prolonged and sustained release for up to a week. In vitro viability assay performed against breast cancer cells demonstrated that the nanoencapsulation of DOX modulated the cytotoxicity of the bioactive as a function of the incubation time with respect to the free form of the drug. The results demonstrate the potential use of GNPs as carriers of hydrophilic antitumor compounds.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gliadin Nanoparticles Containing Doxorubicin Hydrochloride: Characterization and Cytotoxicity

et al. 2023

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Complex bio‐nanoparticles assembled by a pH‐driven method: environmental stress stability and oil–water interfacial behavior

Chen,

Luo

et al. 2023

J Sci Food Agric

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BackgroundProtein‐based nanoparticles have gained considerable interest in recent years due to their biodegradability, biocompatibility, and functional properties. Nevertheless, However, nanoparticles formed from hydrophobic proteins are prone to instability under environmental stress, which restricts their potential applications. Therefore, it is of great importance to develop green approaches for fabricating and improving the physicochemical performance of hydrophobic protein‐based nanoparticles.ResultsGliadin/shellac complex nanoparticles (168.87~403.67 nm) with various gliadin/shellac mass ratios (10:0 ~ 5:5) were prepared using a pH‐driven approach. Compared to gliadin nanoparticles, complex nanoparticles have shown enhanced stability against neutral pH, ions, and boiling. They remained stable under neutral conditions, at NaCl concentrations ranging from 0 to 100 mmol/L, and even when boiled at 100 °C for 90 min. These nanoparticles were capable of effectively reducing the oil‐water interfacial tension (5~11 mN/m), but a higher shellac amount in nanoparticles compromised their ability to lower the interfacial tension. Moreover, the wettability of nanoparticles changed as the gliadin/shellac mass ratio, leading to a range of three‐phase contact angles from 52.41° to 84.85°. Notably, complex nanoparticles with a gliadin/shellac mass ratio of 8:2 (G/S 8:2) exhibited the maximum contact angle (84.85°), along with the highest emulsifying activity (52.42 m2/g) and emulsifying stability (65.33%).ConclusionsThe findings of the study revealed that gliadin/shellac complex nanoparticles exhibited excellent resistance to environmental stress and demonstrated superior oil‐water interfacial behavior, which have great potential for further development as food emulsifiers or as nano‐delivery systems for nutraceuticals.This article is protected by copyright. All rights reserved.

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Protein Nanocarriers for the Delivery of Phytoconstituents

Solanki,

Patel

2024

Nanotechnology Based Delivery of Phytoconstituents and Cosmeceuticals

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Ascorbic acid-loaded gliadin nanoparticles as a novel nutraceutical formulation

Cited by 16 publications

References 93 publications

Gliadin Nanoparticles Containing Doxorubicin Hydrochloride: Characterization and Cytotoxicity

Gliadin Nanoparticles Containing Doxorubicin Hydrochloride: Characterization and Cytotoxicity

Complex bio‐nanoparticles assembled by a pH‐driven method: environmental stress stability and oil–water interfacial behavior

Protein Nanocarriers for the Delivery of Phytoconstituents

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