Ascorbic Acid Distribution in Three Introgression Lines of Tomato

Minutolo, Maria; Matteo, Antonio Di; Amalfitano, Carmine; Evidente, Antonio; Monti, L. M.; Errico, A.

doi:10.5539/jas.v2n3p75

Cited by 2 publications

(3 citation statements)

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“…Tomato fruits represent a relevant source of ascorbic acid in the human diet. Previous studies identified two ILs with QTLs for ascorbic acid [16,[21][22][23]. In this work, we sought to examine possible differences in the ascorbic acid accumulation among these lines in controlled plant tissue culture conditions, in relation to the maturity stage of the fruit used for the explants and the age of the callus.…”

Section: Discussionmentioning

confidence: 99%

“…The ascorbic acid content was estimated on fruit pericarp and after 15, 30, 45 and 60 days of in vitro culture from ten randomly chosen calli for each experimental thesis. Samples were taken during the first hours of the light period, weighted, frozen in liquid nitrogen and stored at −80 • C. Ascorbic acid was extracted as reported by [23] and it was measured as previously described [13,37]. AsA quantities were determined by subtracting the reduced AsA (rAsA) from the total Asa (tAsA).…”

Section: Ascorbic Acid Extraction and Quantificationmentioning

confidence: 99%

“…A widely used IL-population derived from crossing the cultivated variety M82 with S. pennelli [20]. Different studies identified two lines (IL7-3 and IL12-4) that harbour QTLs for AsA accumulation [16,[21][22][23]. Although ILs are an important experimental tool in genetics and breeding, they are not designed to be cultivated [24].…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of Ascorbic Acid in Tomato Cell Culture: Influence of the Genotype, Source Explant and Time of In Vitro Cultivation

Minutolo¹,

Chiaiese²,

Matteo³

et al. 2020

Antioxidants

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The production and commercialization of natural antioxidants is gaining increasing importance due to their wide range of biological effects and applications. In vitro cell culture is a valuable source of plant bioactive compounds, especially those highly dependent on environmental factors. Nonetheless, research on the accumulation in plant cultured cells of water-soluble antioxidant vitamins, such as the ascorbic acid (AsA), is very limited. Tomato fruits are a main dietary source of vitamin C and in this work, we explored the potential of in vitro cultured cells for AsA accumulation. Specifically, using a full factorial design, we examined the effect of the source explant, the time in tissue culture and the genetic difference present in two Introgression Line (IL7-3 and IL12-4) that harbor Quantitative Trait Loci (QTLs) for ascorbic acid in fruits. Moreover, we performed an expression analysis of genes involved in AsA metabolism to highlight the molecular mechanisms that can account for the difference between fruit explants and calli. Our work indicated that cultured tomato cells accumulate AsA well beyond the amount present in fruits and that the three factors under investigation and their interaction significantly influence AsA accumulation. The time in tissue culture is the main single factor and, different from the expectations for secondary metabolites, explants from unripe, mature green fruits provided the highest increase in AsA. Moreover, in controlled conditions the genetic differences between the ILs and the control genotype are less relevant for calli cultivated for longer time. Our work showed the potential of tomato cell culture to produce AsA and prompt further refinements towards its possible large-scale exploitation.

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Section: Discussionmentioning

confidence: 99%

Section: Ascorbic Acid Extraction and Quantificationmentioning

confidence: 99%

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Accumulation of Ascorbic Acid in Tomato Cell Culture: Influence of the Genotype, Source Explant and Time of In Vitro Cultivation

Minutolo¹,

Chiaiese²,

Matteo³

et al. 2020

Antioxidants

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Polyphenol distribution in plant organs of tomato introgression lines

Minutolo¹,

Amalfitano²,

Evidente³

et al. 2013

Natural Product Research

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The content of total polyphenols, chlorogenic, caffeic (CaA) and ferulic acids, and rutin, was investigated in plant organs of three introgression lines (IL7-3, IL10-1 and IL12-4) of Solanum pennellii in Solanum lycopersicum cv M82 and compared with that of cropped parental. Such study aims to evidence factors associated to the introgressions that can affect polyphenol distribution in plant. Among genotypes few differences in polyphenols were recorded on fresh weight basis. IL7-3 showed higher total polyphenols in fruits and lower rutin in leaves than the other genotypes. IL12-4 showed an increasing trend of total polyphenol concentration in fresh vegetative organs; however, this seems to depend on the lower water content rather than on a higher polyphenol biosynthesis in the genotype. IL10-1 sowed higher CaA and lignin contents in leaves. Such differences agree with the morphological and physiological traits of the genotypes.

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Ascorbic Acid Distribution in Three Introgression Lines of Tomato

Cited by 2 publications

References 40 publications

Accumulation of Ascorbic Acid in Tomato Cell Culture: Influence of the Genotype, Source Explant and Time of In Vitro Cultivation

Accumulation of Ascorbic Acid in Tomato Cell Culture: Influence of the Genotype, Source Explant and Time of In Vitro Cultivation

Polyphenol distribution in plant organs of tomato introgression lines

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