Polyphenol distribution in plant organs of tomato introgression lines

Abstract

The content of total polyphenols, chlorogenic, caffeic (CaA) and ferulic acids, and rutin, was investigated in plant organs of three introgression lines (IL7-3, IL10-1 and IL12-4) of Solanum pennellii in Solanum lycopersicum cv M82 and compared with that of cropped parental. Such study aims to evidence factors associated to the introgressions that can affect polyphenol distribution in plant. Among genotypes few differences in polyphenols were recorded on fresh weight basis. IL7-3 showed higher total polyphenols in fruits and lower rutin in leaves than the other genotypes. IL12-4 showed an increasing trend of total polyphenol concentration in fresh vegetative organs; however, this seems to depend on the lower water content rather than on a higher polyphenol biosynthesis in the genotype. IL10-1 sowed higher CaA and lignin contents in leaves. Such differences agree with the morphological and physiological traits of the genotypes.

Effect of regulated deficit irrigation on quality parameters, carotenoids and phenolics of diverse tomato varieties (Solanum lycopersicum L.)

“…In some cases, the TPC in cherry varieties (ranging from 209 to 350 mg/100 g DW) were higher and in others similar to those reported in common tomatoes (ranging from 210 to 286 mg/100 g DW). These data agree well with those of other authors, for example Vinha et al (2014) (Vinha, Alves, Barreira, Castro, & Costa, 2014), who studied four tomato cultivars and reported TPC values between 54 to 67 mg gallic acid equivalent (GAE)/100 g On the other hand, TPC in common varieties in this study were higher compared with those found in others, which reported variations of concentration from 18 to 31 mg GAE/100 g FW (Gahler, Otto, & Bphm, 2003) or from 26 to 42 mg/100 g FW (Minutolo, Amalfitano, Evidente, Frusciante, & Errico, 2013). This may be due, at least in part, to the fact that in this study acidified methanol was used and this favors the extraction of phenolics according to the findings of some authors (Ju & Howard, 2003;Arranz, 2010).…”

“…For example Pernice et al (2010) studied two tomato cherry varieties in normal, reduced and lack of irrigation and concluded that the varieties had higher contents of phenolics in reduced irrigation, while a non consistent behaviour was observed for the non irrigated samples (Pernice R. , et al, 2010); this behavior was also noticed in other studies (Sánchez-Rodríguez R. , Leyva, Constán-Aguilar, Romero, & Ruiz, 2012; Sánchez-Rodríguez, Ruiz, Ferreres, & Moreno, 2012). Again, the data demonstrate that the genotype has an important effect on the levels of the compounds (Minutolo, Amalfitano, Evidente, Frusciante, & Errico, 2013).…”

“…In cherry varieties, the treatment caused an overall reduction in the total phenolic content, although in ʽByelsaʼ it increased. Phenolic compounds are thought to have a strong link to the cultivar and water content of the fruit (Minutolo, Amalfitano, Evidente, Frusciante, & Errico, 2013;Sánchez-Rodríguez, Ruiz, Ferreres, & Moreno, 2012), which could explain in part these results. On the other hand, these data agree well with those of other authors.…”

“…This could be due to some extent to the fact that phenolics are components of plant tissues, and their distribution is influenced by light and environmental conditions. As an example, some studies suggest that leaves growing under shade have a low concentration of phenolics (Minutolo, Amalfitano, Evidente, Frusciante, & Errico, 2013;Rugna, Ricco, Gurni, & Wagner, 2008), which may help explain, at least in part, the high TPC in the CIII cluster.…”

“…These observations contrasted with those of other authors. For example Minutolo et al (2013) reported that chlorogenic acid levels decreased at the top of the plant (Minutolo, Amalfitano, Evidente, Frusciante, & Errico, 2013). Again these differences could also be related with changes in the environmental conditions and harvest.…”