Ascaridoid nematodes of teleostean fishes from the eastern north Atlantic and seas of the north of Europe

Petter, A. J.; Cabaret, Jacques; Tcheprakoff, R.

doi:10.1051/parasite/199502s2217

Cited by 38 publications

(28 citation statements)

References 8 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The differences among some data obtained in this study and those obtained by other authors (Yoshinaga 44 (Hartwich 1975, Petter & Cabaret 1995. It could also be due to the occurrence of sibling species of H. aduncum, suggested by Martín-Sánchez et al (1998), as occurs in other anisakids.…”

Section: Resultscontrasting

confidence: 82%

“…Hatched, ensheathed larvae lived for over 3 to 4 mo at 13°C, but without apparent development. Other authors reported larval survival at 5°C for over 5 mo (Balbuena et al 1998), or for 1 wk at 20°C (Køie 1993), after hatching and remaining ensheathed.The differences among some data obtained in this study and those obtained by other authors (Yoshinaga 44 (Hartwich 1975, Petter & Cabaret 1995. It could also be due to the occurrence of sibling species of H. aduncum, suggested by Martín-Sánchez et al (1998), as occurs in other anisakids.…”

contrasting

confidence: 82%

“…1987, Køie 1993, Balbuena et al 1998, González 1998) may be due to the occurrence of subspecies of Hysterothylacium aduncum (Hartwich 1975, Petter & Cabaret 1995. It could also be due to the occurrence of sibling species of H. aduncum, suggested by Martín-Sánchez et al (1998), as occurs in other anisakids.…”

Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

“…The worms were observed individually under an inverted microscope, and those that showed any kind of internal or external damage were discarded. They were then identified according to morphological features (Hartwich 1975, Petter & Maillard 1988, Petter & Cabaret 1995.Prior to cultivation, each larva was placed in an antibiotic-antifungal solution (80 mg gentamicin sulfate, 0.625 mg amphotericin B, 10 000 IU sodium penicillin G, 10 mg streptomycin sulfate and 4.5 ml Hanks' solution, for a final solution volume of 10 ml) and axenized as described elsewhere (Iglesias et al 1997, Iglesias et al 2001. Worms were cultured in sterile polystyrene 30 ml flasks.…”

mentioning

confidence: 99%

See 4 more Smart Citations

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

Adroher¹,

Malagón²,

Valero³

et al. 2004

Dis. Aquat. Org.

View full text Add to dashboard Cite

Anisakids are parasitic nematodes of fish worldwide, producing economic and human health concerns. It is thus important to ascertain their in vitro life cycle in laboratory studies. Here we describe the in vitro development of third-stage larvae (L3) of Hysterothylacium aduncum isolated from blue whiting Micromesistius poutassou, to the hatching of L3 from eggs obtained from H. aduncum worms grown in GLIT medium (a modified mixture of Yaeger's LIT [Liver Infusion Tryptose] and Grace's media) at pH 4.0, 13°C and with 5% CO 2 in air. Under these conditions, L3 recovered from fish developed to mature adults (3.4 to 6.2 cm in length), with oviposition starting from Day 26 in culture. Fertilized eggs (mean size 64 × 52 µm) had a thick, rugose eggshell and were larger than unfertilized ones (mean size 49 × 42 µm), whose eggshells were thin and smooth. Eggs laid during the first and second week of oviposition, and maintained in 2.8% NaCl solution at 13°C, developed to L3. Under these maintenance conditions, between 20.6 and 52.5% of the eggs laid during the first week developed into larvae. Motile larvae, enclosed in a sheath, hatched from between 2 and 11% of these eggs. The larvae started to hatch 23 d after deposition. These larvae were 144 to 215 µm in length, enclosed in a 237 to 305 µm-long sheath. This GLIT culture medium may help to study the biology of this and other anisakids.KEY WORDS: Fish parasite · Hysterothylacium aduncum · Nematoda · Anisakidae · In vitro culture · Egg development · Hatching Resale or republication not permitted without written consent of the publisherDis Aquat Org 58: [41][42][43][44][45] 2004 mesistius poutassou (blue whiting), family Gadidae, purchased from the fish market of Granada (southern Spain). Blue whiting, on the Atlantic and Mediterranean Spanish coasts, are frequently parasitized by H. aduncum (Ruiz-Valero et al. 1992, Valero et al. 2000. The worms, found free in the host body cavity, were collected with the help of a needle with a blunt tip, placed on a Petri dish and washed in 0.9% NaCl solution several times. The worms were observed individually under an inverted microscope, and those that showed any kind of internal or external damage were discarded. They were then identified according to morphological features (Hartwich 1975, Petter & Maillard 1988, Petter & Cabaret 1995.Prior to cultivation, each larva was placed in an antibiotic-antifungal solution (80 mg gentamicin sulfate, 0.625 mg amphotericin B, 10 000 IU sodium penicillin G, 10 mg streptomycin sulfate and 4.5 ml Hanks' solution, for a final solution volume of 10 ml) and axenized as described elsewhere (Iglesias et al. 1997, Iglesias et al. 2001. Worms were cultured in sterile polystyrene 30 ml flasks. The culture medium (10 ml) was placed into each flask with 8 to 10 parasites. The culture flasks were then placed in an incubator at 13°C and 5% CO 2 in humid air, and the culture medium was renewed once a week. The worms were observed daily for motility, moulting and survival. The culture medium (GLI...

show abstract

Section: Resultscontrasting

confidence: 82%

contrasting

confidence: 82%

Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

Adroher¹,

Malagón²,

Valero³

et al. 2004

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

Identification of anisakid nematodes from the Southern Baltic Sea using PCR-based methods

Szostakowska

Myjak

Kur

2002

Molecular and Cellular Probes

View full text Add to dashboard Cite

Accumulation of Some Heavy Metals Seasonally in Hysterotylacium aduncum (Nematoda) and Its Host Red Sea Bream, Pagellus erythrinus (Sparidae) from Gulf of Iskenderun (North-Eastern Mediterranean)

Dural

Genc

Yemenicioglu

et al. 2009

Bull Environ Contam Toxicol

View full text Add to dashboard Cite

The Red Sea Bream's nematode and Sparus aurata, sampled from the Iskenderun Bay, North-eastern Mediterranean in March 2008 were analysed by Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) for their some heavy metal (Cd, Cr, Cu, Fe, Hg, Mn, Mg, Pb and Zn) levels. The metal concentrations of the parasites were compared to different organs (liver, muscle, swimbladder, intestine and skin) of the fish hosts. The highest Cd (0.303 mg/kgg ww) concentrations were found in the muscle, highest Cr (4.932 mg/kg ww), Hg (2.350 mg/kg ww) Pb (22.82 mg/kg ww) concentrations were found in the parasite, highest Cu (7.608 mg/kg ww) and Fe (176.7 mg/kg ww) concentrations were found in the liver, highest Mn (31.24 mg/kg ww) Zn (78.51 mg/kg ww) concentrations were found in the swimbladder for parasitized fish. The highest Cd (0.612 mg/kg ww), Cu (8.261 mg/kg ww) Fe (261.1 mg/kg ww) concentrations were found in the liver, highest Cr (6.123 mg/kg ww) and Pb (9.125 mg/kg ww) concentrations were found in the intestine, highest Hg (2.013 mg/kg ww) Zn (83.30 mg/kg ww) and Mn (41.24 mg/kg ww) concentrations were found in the swimbladder for un-parasitized fish.

show abstract

Ascaridoid nematodes of teleostean fishes from the eastern north Atlantic and seas of the north of Europe

Cited by 38 publications

References 8 publications

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

In vitro development of the fish parasite Hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg

Identification of anisakid nematodes from the Southern Baltic Sea using PCR-based methods

Accumulation of Some Heavy Metals Seasonally in Hysterotylacium aduncum (Nematoda) and Its Host Red Sea Bream, Pagellus erythrinus (Sparidae) from Gulf of Iskenderun (North-Eastern Mediterranean)

Contact Info

Product

Resources

About