Identification of anisakid nematodes from the Southern Baltic Sea using PCR-based methods

Szostakowska, Beata; Myjak, P; Kur, J

doi:10.1006/mcpr.2001.0391

Cited by 42 publications

(36 citation statements)

References 15 publications

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“…is frequently recovered from many species of fish and fish-eating birds (e.g. Torres et al 1991;Yang and Liao 1997;Torres et al 2000;Yang et al 2000Yang et al , 2001Abollo et al 2001;Barus et al 2001;Kijewska et al 2002;Dezfuli et al 2002;Szostakowska et al 2002;Barson and Marshall 2004), SSCPbased screening of the ITS-1 and/or ITS-2 rDNA will be a useful approach for elucidating their life cycles and ecology in Europe, and for the specific identification of anisakids considered to represent C. rudolphii s.l.) from other geographical locations, such as China (e.g.…”

Section: Resultsmentioning

confidence: 98%

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Genetic evidence for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) and the validity of Contracaecum septentrionale (Kreis, 1955) (Nematoda: Anisakidae)

D’Amelio

Paggi

et al. 2005

Parasitol Res

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Specimens of Contracaecum rudolphii sensu lato (s.l.) (Nematoda: Anisakidae) from Phalacrocorax carbo sinensis from northeastern and central Italy were characterised genetically and compared with those from Phalacrocorax aristotelis from Galician coasts, Spain (identified as C. rudolphii A by multilocus enzyme electrophoresis) and with specimens of C. septentrionale from Alca torda from the Galician coasts, Spain. The first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of ribosomal DNA (rDNA) were amplified by polymerase chain reaction (PCR) from individual nematodes and the amplicons subjected to single-strand conformation polymorphism (SSCP) analysis and/or sequencing. For each ITS region, C. septentrionale specimens were distinct from those of C. rudolphii (s.l.) and C. rudolphii A based on SSCP profiles and ITS sequences. Some specimens of C. rudolphii (s.l.) had the same SSCP profiles and ITS sequences as C. rudolphii A, whereas the others had distinct SSCP profiles and ITS sequences and were suggested to represent C. rudolphii B based on host and geographical origins and genetic similarity to C. rudolphii A. While no length or nucleotide variation in the ITS-1 and ITS-2 sequences was detected within each taxon, nucleotide differences of 1.8-5.5% (ITS-1) and 5.1-12.2% (ITS-2) were detected among them. The results support the hypothesis that C. rudolphii represents a complex of at least two sibling species and provide support for the validity of C. septentrionale as a separate species. The definition of genetic markers in the ITS rDNA provides opportunities for investigating the life cycles, transmission patterns and ecology of the anisakid nematodes studied herein.

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Section: Resultsmentioning

confidence: 98%

“…Hartwich 1964;Torres et al 1991;Yang and Liao 1997;Torres et al 2000;Yang et al 2000Yang et al , 2001Abollo et al 2001; Barus et al 2001;Kijewska et al 2002;Dezfuli et al 2002;Szostakowska et al 2002; Barson and Marshall 2004). The species was found by Hartwich (1964) as nom.…”

Section: Introductionmentioning

confidence: 88%

Genetic evidence for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) and the validity of Contracaecum septentrionale (Kreis, 1955) (Nematoda: Anisakidae)

D’Amelio

Paggi

et al. 2005

Parasitol Res

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“…in Antarctic fish (Kl€ oser et al 1992;Palm 1999;Palm et al 1994Palm et al , 1998Palm et al , 2007 or the frequent infestation of the Atlantic and Baltic Sea herring (e.g. Szostakowska et al 2002;Levsen and Lunestad 2010) with A. simplex (s.s.) has no visible effect on the host's fitness. Some fish populations are commonly associated with very high anisakid burden, closely related to the abundance of their final hosts in the area of investigation (e.g.…”

Section: Pathogenicity and Zoonotic Potentialmentioning

confidence: 99%

Anisakid Nematode (Ascaridoidea) Life Cycles and Distribution: Increasing Zoonotic Potential in the Time of Climate Change?

Klimpel

Palm

2011

Progress in Parasitology

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“…A. simplex (sensu stricto) is the only sibling species of the A. simplex complex present in the Baltic Sea (Kijewska et al, 2000;Nascetti et al, 1986;Skov et al, 2009;Szostakowska et al, 2002).…”

Section: Anisakis Simplex (Sensu Stricto)mentioning

confidence: 99%

“…In Table 7 data on the distribution of C. osculatum in different fish species in the Baltic Sea are reported. (Szostakowska et al, 2002(Szostakowska et al, , 2005 Crucian carp, Caspian round goby * n.a. (Szostakowska and Fagerholm, 2007)* * C. rudolphii B, can develop both in fresh and brackish water n.a.…”

Section: Contracaecum Osculatum (Sensu Stricto)mentioning

confidence: 99%

Scientific Opinion on assessment of epidemiological data in relation to the health risks resulting from the presence of parasites in wild caught fish from fishing grounds in the Baltic Sea

2011

EFS2

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For fishery products caught from fishing grounds in the Baltic Sea, four groups of viable parasites present possible health risks, Anisakis simplex (sensu stricto), Contracaecum osculatum (sensu stricto), Pseudoterranova decipiens (sensu stricto) and Diphyllobothrium spp. Since A. simplex and Pseudoterranova decipiens have been found in fishery products in International Council for the Exploration of the Sea (ICES) subdivisions 22, 23, 24, 25, 26, public health risks due to the presence of these parasites cannot be excluded in any fishery products caught from these areas. Migrating fish from areas where A. simplex, and to a lesser degree P. decipiens, occur may carry these parasites and reach the northern Baltic, therefore, public health risks due to parasites in all migrating fish (including salmon) in Baltic Sea cannot be excluded. C. osculatum occurs in fish throughout all areas of the Baltic Sea. However, at present it is not possible to assess the public health importance of viable C. osculatum larvae in fishery products. Diphyllobothrium spp. occurs in fish species in brackish waters of Baltic Sea. Hence all freshwater fish as well as migrating fish, including sea trout and whitefish, are of public health importance if consumed raw, since they may carry viable parasites. More research is needed to elucidate the importance of C. osculatum from fish as a source of human infection, including pathogenicity of this parasite and the anatomic distribution of the parasite in edible parts of the fish. In order to definitively identify species of anisakids, genetic/molecular methods should be more widely applied to material from all hosts of the Baltic Sea. Surveillance of anisakiasis and other parasitic infections in the human population in Baltic Sea countries should be improved.

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Identification of anisakid nematodes from the Southern Baltic Sea using PCR-based methods

Cited by 42 publications

References 15 publications

Genetic evidence for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) and the validity of Contracaecum septentrionale (Kreis, 1955) (Nematoda: Anisakidae)

Genetic evidence for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) and the validity of Contracaecum septentrionale (Kreis, 1955) (Nematoda: Anisakidae)

Anisakid Nematode (Ascaridoidea) Life Cycles and Distribution: Increasing Zoonotic Potential in the Time of Climate Change?

Scientific Opinion on assessment of epidemiological data in relation to the health risks resulting from the presence of parasites in wild caught fish from fishing grounds in the Baltic Sea

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