Artificial Substrate Bioassay for Testing Oviposition of Southern Green Stink Bug Conditioned by Soybean Plant Chemical Extracts

Oviposition preference of the neotropical brown stink bug Euschistus heros on artificial substrates of different colors

“…Furthermore, it can be tested the synergistic effect of the color of the artifi cial substrate with specifi c chemical attractive (aggregation pheromones, for example) to E. heros (RENWICK, 1989;MORAES, et al, 2008;BORGES et al, 2010) as well as utilize soybean plants extracts (PANIZZI et al, 2004) on artifi cial substrates to verify whether these compounds can further optimize the eggs production.…”

“…However, the major challenge to rear stink bugs in the laboratory is to provide adequate substrates of oviposition to concentrate postures and reduce the time of handling during the maintenance of colonies. Studies using artifi cial oviposition substrates with different species of stink bugs show that these insects can lay eggs on paper towel hanging inside cages (SHEARER & JONES, 1996), in voil (BUNDY & McPHERSON, 2000), on plastic structures that mimic soybean leaves (PANIZZI et al, 2000b) on cheesecloth stretched treated with chemical extract of soybean pods (PANIZZI et al, 2004), on wool yarn, (SILVA & PANIZZI, 2008), and also on cotton balls (SILVA & PANIZZI, 2007). All these artifi cial substrates have proved to be appropriate for oviposition of several pentatomids species, but not evaluated the color of the substrates as attractive characteristics to stink bugs.…”

The role of signals of different modalities in initiating vibratory communication in Nezara viridula

“…The role of plantoriginating chemical signals in inducing vibratory signals described as spontaneous singing can not be excluded as it has been shown to affect insect behaviour [19]. In Nezara viridula plant chemicals affect oviposition [20] but their direct triggering of vibrational communication has not yet been studied. Spontaneous singing has been overlooked until now, especially in males, but our experiments show it to occur in a high proportion of males and approximately half as much in females in daylight.…”

Use of artificial substrates of different colors for oviposition by the brown stink bug Euschistus heros (Hemiptera, Pentatomidae)

“…Among earlier experiments, Panizzi et al (2000b), studying the southern green stink bug Nezara viridula (L.), demonstrated for the first time that a model simulating a natural plant can be used as substrate for oviposition along with an artificial diet. Later, Panizzi et al (2004) successfully used cheesecloth treated with soybean plant extracts for oviposition of this stink bug. Silva & Panizzi (2007) expanded the dataset on the use of artificial substrates by evaluating the oviposition of seven species of pentatomids on cotton balls.…”