“Artificial micro organs”—a microfluidic device for dielectrophoretic assembly of liver sinusoids

Schütte, Julia; Hagmeyer, Britta; Holzner, Felix; Kubon, M.; Werner, S.; Freudigmann, Christian; Benz, Karin; Böttger, J; Gebhardt, Rolf; Becker, Holger; Stelzle, Martin

doi:10.1007/s10544-011-9517-7

Cited by 86 publications

(79 citation statements)

References 20 publications

(19 reference statements)

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“…This line of research has produced notable results as well. [ 17 ] Schütte et al [ 18 ] used this approach, in addition to developing a microfl uidic test system for liver toxicity. This system is an organ-like liver 3D co-culture of hepatocytes and endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Caplin

Granados

James

et al. 2015

Adv Healthcare Materials

178

124

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In recent years, the exploitation of phenomena surrounding microfluidics has seen an increase in popularity, as researchers have found a way to use their unique properties to create superior design alternatives. One such application is representing the properties and functions of different organs on a microscale chip for the purpose of drug testing or tissue engineering. With the introduction of "organ-on-a-chip" systems, researchers have proposed various methods on various organ-on-a-chip systems to mimic their in vivo counterparts. In this article, a systematic approach is taken to review current technologies pertaining to organ-on-a-chip systems. Design processes with attention to the particular instruments, cells, and materials used are presented.

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Section: Introductionmentioning

confidence: 99%

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Caplin

Granados

James

et al. 2015

Adv Healthcare Materials

178

124

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“…This feature of DEP was also demonstrated in the previous studies. 18,19 The cell membranes of dead cells are permeable, and allow the ionic concentrations of the intra-and extracellular solutions to equilibrate. Because dielectrophoretic forces depend on a difference in ionic conductivity, dead cells are not affected by dielectrophoresis.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic chip system for the selection and enrichment of cell binding aptamers

Stoll¹,

Kiessling

Stelzle

et al. 2015

Biomicrofluidics

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Aptamers are promising cell targeting ligands for several applications such as for the diagnosis, therapy, and drug delivery. Especially, in the field of regenerative medicine, stem cell specific aptamers have an enormous potential. Using the combinatorial chemistry process SELEX (Systematic Evolution of Ligands by Exponential enrichment), aptamers are selected from a huge oligonucleotide library consisting of approximately 10 15 different oligonucleotides. Here, we developed a microfluidic chip system that can be used for the selection of cell specific aptamers. The major drawbacks of common cell-SELEX methods are the inefficient elimination of the unspecifically bound oligonucleotides from the cell surface and the unspecific binding/uptake of oligonucleotides by dead cells. To overcome these obstacles, a microfluidic device, which enables the simultaneous performance of dielectrophoresis and electrophoresis in the same device, was designed. Using this system, viable cells can be selectively assembled by dielectrophoresis between the electrodes and then incubated with the oligonucleotides. To reduce the rate of unspecifically bound sequences, electrophoretic fields can be applied in order to draw loosely bound oligonucleotides away from the cells. Furthermore, by increasing the flow rate in the chip during the iterative rounds of SELEX, the selection pressure can be improved and aptamers with higher affinities and specificities can be obtained. This new microfluidic device has a tremendous capability to improve the cell-SELEX procedure and to select highly specific aptamers. V C 2015 AIP Publishing LLC. [http://dx

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“…Additionally, a microfluidic test system capable of 3D co-culturing with DEP was also reported. 79 This microfluidic system featuring cell chambers and interdigitated electrodes was used for the 3D co-culture of hepatocytes and endothelial cells in multiple chambers for liver toxicity assessments.…”

Section: Cell Manipulation Using Dielectrophoresis (Dep)mentioning

confidence: 99%

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

Ino

Şen

Shiku

et al. 2017

Analyst

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Herein, we present an overview of recent research progress in the development of micro/nanoelectrochemical probe and chip devices for the evaluation of three-dimensional (3D) cultured cells. First, we discuss probe devices: a general outline, evaluation of O 2 consumption, enzyme-modified electrodes, evaluation of endogenous enzyme activity, and the collection of cell components from cell aggregates are discussed. The next section is focused on integrated chip devices: a general outline, electrode array devices, smart electrode array devices, droplet detection of 3D cultured cells, cell manipulation using dielectrophoresis (DEP), and electrodeposited hydrogels used for fabrication of 3D cultured cells on chip devices are discussed. Finally, we provide a summary and discussion of future directions of research in this field.

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“Artificial micro organs”—a microfluidic device for dielectrophoretic assembly of liver sinusoids

Cited by 86 publications

References 20 publications

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Microfluidic chip system for the selection and enrichment of cell binding aptamers

Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells

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