Artificial metalloenzymes as catalysts in non-natural compounds synthesis

Artificial Metalloproteins for Binding and Stabilization of a Semiquinone Radical

“…A 2 mL portion (500× dilutions) of overnight culture was used to inoculate at 37 °C 500 mL of fresh LB medium containing 100 μg/mL of ampicillin and 34 μg/mL of chloramphenicol. When the culture reached an optical density at 600 nm of 0.8–0.9, the expression was induced with isopropyl β- d -1-thiogalactopyranoside (IPTG) (final concentration 1 mM) and l -arabinose (final concentration 0.02%), and 100 mg/L of bpyA (racemic mixture, for synthesis see ref ( 2 )) was added. Expression was done overnight at 30 °C.…”

Recent advances in metallopolymer-based drug delivery systems

“…Their properties can be adjusted by changing the interaction strength of metal centers with the polymer backbone. [44][45][46][47][48] MPs are highly interesting materials with properties combining the technological advantages of polymers with the functionality of metal centers, therefore, these compounds have outstanding potential for use in a broad range of advanced sustainable applications including functional materials, [49][50][51][52][53][54][55] catalysis and biocatalysis, [56][57][58][59][60][61][62][63] electroactive, 64 photoactive, [65][66][67][68][69][70] conducting 71 and magnetic 45 materials, as well as environment 72 and nanomaterials science. [73][74][75][76][77][78][79][80] MPs have been known for a long time but have recently become the subject of large development.…”

Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

“…Enzymes play an essential role in the production of biofuels, and redox‐active metalloenzymes play a particularly vital role in hydrogen generation, methane production, and a recently discovered role in cellulose breakdown . Other redox‐enzyme based applications range from the development of novel biocatalysts for solving challenging synthetic problems, to the sequestering of atmospheric CO 2 . As both redox‐active proteins and enzymes can be used to elicit an electronic response from a biological stimulus, there is also a vast range of literature exploring the use of such molecules for developing new sensor technologies .…”