With the rapid development of Internet technology and artificial intelligence, information technology has shown a trend of exponential growth. To improve the core competitiveness, the organization's boundaries gradually disappear and move towards virtualization. Virtualization is an effective form of organizational development in the information age. Based on the literature review regarding artificial intelligence and the related theories of organizational virtualization, this paper focuses on analysing the phased impact of the application of artificial intelligence on the virtualization of internal operations and the virtualization of interorganizational cooperation.