Artificial DNA and surface plasmon resonance

D’Agata, Roberta; Spoto, Giuseppe

doi:10.4161/adna.21383

Cited by 26 publications

(14 citation statements)

References 98 publications

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“…We employed a nanoparticle‐enhanced strategy to develop a sandwich assay able to provide an ultrasensitive detection of aureus strain Mu50 and Listeria m gDNA. The assay exploited the superior performances of PNA probes in targeting complementary DNA sequences, arising from the higher thermal stability of PNA/DNA complexes compared to DNA/DNA duplexes ,…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive Detection of Staphylococcus aureus and Listeria monocytogenes Genomic DNA by Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

2017

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The rapid, sensitive and specific detection of pathogenic bacteria is key to the prevention of a variety of foodborne illnesses representing a significant threat to public health. Conventional methods for the detection and identification of pathogens may take up to a few days to yield an answer, and for this reason, efforts have been made to develop new biosensing platforms for the efficient detection of foodborne pathogens. Here we report the use of a nanoparticle‐enhanced surface plasmon resonance (SPR) imaging biosensor for the rapid and femtomolar‐attomolar detection of Staphylococcus aureus and Listeria monocytogenes genomic DNAs. We performed the nanoparticle enhanced SPR imaging detection of pathogen genomic DNAs with no need for the polymerase chain reaction (PCR) pre‐amplification of the sequences to be detected. Here we show that peptide nucleic acid (PNA) probes can discriminate between two pathogenic genomic DNAs. We tested the selectivity of the detection method against control probe sequences or contaminating bovine genomic DNA and demonstrated, for the first time, the attomolar detection of Staphylococcus aureus genomic DNA in the presence of a 10‐fold higher concentration of bovine genomic DNA.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive Detection of Staphylococcus aureus and Listeria monocytogenes Genomic DNA by Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

2017

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“…Various bioreceptors have been used including antibodies (monoclonal, polyclonal and recombinant), Fab antibody fragments, single-chain antibody fragments, antibody mimmetics such as affibodies, recombinant proteins, peptides, carbohydrates, small organic molecules, DNA, RNA, oligonucleotides and recently even artificial DNA [34]. Typically antibodies offering high affinity and specificity are the most widely used bioreceptors for different types of applications.…”

Section: Biorecognition Elementsmentioning

confidence: 99%

Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

et al. 2014

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Surface plasmon resonance sensors have made vast advancements in the sensing technology and the number of applications achievable. New developments in surface plasmon resonance sensors have gained considerable momentum promoted by the urgent needs of fast, reliable and label-free methods for detection and quantification of analytes in molecular biology, medicine and other life sciences. However, even if enormous improvements in the limits of detections have been achieved, this technology still faces important challenges to be translated to clinical practice or in-field measurements. This paper reviews the important recent advances of this technology for the label-free detection in real biological samples and we discussed the key challenges to be overcome to transit from prototypes to commercial biosensors.

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“…XNAs increase sensor stability by avoiding biodegradation or exploiting improved affinity of XNA versus the target sequence compared with conventional DNA probes. D'Agata et al reviewed works dealing with the great potential of artificial DNA (PNA, LNA, HNA, and MORF) in nucleic acid SPR-based sensing, exhibiting higher selectivity and sensitivity in detecting complementary or mismatching nucleic acid targets [29].…”

Section: Bioreceptorsmentioning

confidence: 99%

Surface plasmon resonance applications in clinical analysis

2014

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In the last 20 years, surface plasmon resonance (SPR) and its advancement with imaging (SPRi) emerged as a suitable and reliable platform in clinical analysis for label-free, sensitive, and real-time monitoring of biomolecular interactions. Thus, we report in this review the state of the art of clinical target detection with SPR-based biosensors in complex matrices (e.g., serum, saliva, blood, and urine) as well as in standard solution when innovative approaches or advanced instrumentations were employed for improved detection. The principles of SPR-based biosensors are summarized first, focusing on the physical properties of the transducer, on the assays design, on the immobilization chemistry, and on new trends for implementing system analytical performances (e.g., coupling with nanoparticles (NPs). Then we critically review the detection of analytes of interest in molecular diagnostics, such as hormones (relevant also for anti-doping control) and biomarkers of interest in inflammatory, cancer, and heart failure diseases. Antibody detection is reported in relation to immune disorder diagnostics. Subsequently, nucleic acid targets are considered for revealing genetic diseases (e.g., point mutation and single nucleotides polymorphism, SNPs) as well as new emerging clinical markers (microRNA) and for pathogen detection. Finally, examples of pathogen detection by immunosensing were also analyzed. A parallel comparison with the reference methods was duly made, indicating the progress brought about by SPR technologies in clinical routine analysis.

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Artificial DNA and surface plasmon resonance

Cited by 26 publications

References 98 publications

Ultrasensitive Detection of Staphylococcus aureus and Listeria monocytogenes Genomic DNA by Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

Ultrasensitive Detection of Staphylococcus aureus and Listeria monocytogenes Genomic DNA by Nanoparticle‐Enhanced Surface Plasmon Resonance Imaging

Surface Plasmon Resonance: Advances of Label-Free Approaches in The Analysis of Biological Samples

Surface plasmon resonance applications in clinical analysis

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