Artificial Biomimicking Matrix Modifications of Nanofibrous Scaffolds by hE-Cadherin-Fc Fusion Protein to Promote Human Mesenchymal Stem Cells Adhesion and Proliferation

Abstract

Extracellular matrix (ECM) plays a fundamental role in regulating cell attachment, proliferation, migration and differentiation. Both synthetic and biologically derived materials have been explored as an ECM in regenerative medicine and tissue engineering. To biomimick the extracellular matrix, we combined the advantages of the biological properties of nanofibrous scaffolds and the fusion protein to apply for the culture of human mesenchymal stem cells in vitro. In this study, we fabricated well random-oriented/aligned nanofibrous scaffolds with PCL, modified with hE-cadherin-Fc fusion protein and studied the synergistic effect of the scaffolds. The random-oriented/aligned architecture was observed in the nanofibrous scaffolds by SEM. XPS and WCA measurements evidenced that hE-cadherin-Fc was successfully modified on the PCL nanofibrous scaffolds and hydrophilicity of the scaffolds was well improved after fusion protein coating. The hE-cadherin-Fc modified markedly promoted the adhesion and proliferation of hMSCs and guided hMSCs to a spindlier morphology compared with unmodified nanofibrous scaffolds. Furthermore, hMSCs on the hE-cadherin-Fc-coated nanofibrous scaffolds also had differentiation potential. These results suggested that the combination of PCL nanofibrous scaffolds and hE-cadherin-Fc fusion protein may be a promising artificial ECM for the behavior of hMSCs in vitro.

The Interactions Between Aligned Poly(L-Lactic Acid) Nanofibers and SH-SY5Y Cells In Vitro

“…The protein expression levels of p-ERK1/2, ERK1/2 and GAPDH in the SH-SY5Y cells that were cultured on PLLA films (control group), aligned PLLA nanofibers (AF group) and aligned PLLA nanofibers with the cell culture medium in addition with 0.5 mg/mL pentapeptide GRGDS (GA group). engineering, [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38] it will be important to investigate the interaction mechanism between cells and materials fabricated by nanotechnologies. We believe that the results shown above might be helpful to guide the design and application of aligned nanofibers in regeneration medicine fields such as nerve tissue engineering.…”

The Role of Nanotechnology in Induced Pluripotent and Embryonic Stem Cells Research

“…259 In addition, partially coordinated motility can be gained after implanting scaffolds that contain neural precursors. 260 of CNT-based thin film, different neuron lineages can be supplied to cell sources, which in turn improves neuron injury repair, compared to single lineage cell transplantation. This demonstrates that CNT-based scaffolds supply a friendly platform for transplanted cells in the harsh injury microenvironment.…”

Human VE-Cadherin Fusion Protein as an Artificial Extracellular Matrix Enhancing the Proliferation and Differentiation Functions of Endothelial Cell

“…Several recombinant proteins have been employed to engineer bioartificial ECM, including structural proteins, growth factors and adhesion proteins that are fused with ECM protein binding domain or immunoglobin G (IgG) Fc domain. − Fc-fusion proteins, which comprise an IgG Fc region linked genetically to the target protein, have shown great promise as chimeric proteins with both functional integrity and long-term stability. , In an Fc-fusion protein, the Fc domain can bind directly to the scaffold surface via hydrophobic interactions, allowing the fused target protein to stretch out and interact with a suitable partner . In our previous work, multiple Fc-fusion proteins, including epithelial (E)-cadherin-Fc, neuronal (N)-cadherin-Fc, and cell growth factor-Fc, were established and used as bioartificial ECM for biomaterial surface modification and shown to successfully provide a biofunctional extracellular microenvironment for regulating cell behavior. ,− …”