Artificial associations between <i>Daucus</i> and nitrogen‐fixing <i>Azotobacter</i> cells in vitro

Varga, S.; Korányi, P.; Preininger, Éva; Gyurján, István

doi:10.1111/j.1399-3054.1994.tb02538.x

Cited by 22 publications

(4 citation statements)

References 11 publications

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“…Due to this ability, we speculated that introduction of Z78 into the plant tissue culture system may benefit the host cells by accelerating and promote better embryoid formation. Several studies were also reported for in vitro Dancus-Azotobacter association (Varga et al, 1994), in vitro association of Strawberry-Azomonas (Preininger et al, 1997), and the most recent study of Crocus sativus Lplant growth promoting rhizobacteria (PGPR) interactions (Parray et al, 2015). However, no observation showed the role of bacteria and the interaction with tissue culture materials of oil palm in the early stage.…”

Section: Resultsmentioning

confidence: 99%

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Lim, S.-L.,

Nabila, H. M. N.,

Atiqah, S.

et al. 2016

MJM

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Aims:The purpose of this experiment was to determine the artificial symbiosis interaction of Herbaspirillum seropedicae (Z78) on oil palm embryogenic calli. Methodology and results: For this purpose, symbiotic associations were established between Z78 and embryogenic calli of oil palm tissue cultured. A total of five treatments involved, in particular: i) + 3.0 mg/L 2,4-D + 100% N MS medium (control), ii) + Z78 pellet cells (1 mL) + 25% N MS medium, iii) + Z78 supernatant (1 mL) + 25% N MS medium, iv) + Z78 broth culture (1 mL) + 25% N MS medium, and v) + Z78 sonicated cells (1 mL) + 25% N MS medium. All treatments were supplied with minimal N sources (25% N), ammonium nitrate and potassium nitrate, while the control was treated with 100% N sources. Treated samples were harvested on D80 and observed for biomass and diameter increment (%), formation of embryoids, and Z78 colonization. The results showed embryogenic calli in the inoculated treatments that contained depleted N produced similar result to the control treatment which contained 100% N nutrients. Positive interactions occurred between the diazotroph and host plant tissues as viewed under FESEM and EFTEM. Among the treatments, Z78 sonicated cell showed better growth of embryogenic calli compared to others. Conclusion, significance and impact study:The in vitro nitrogen-depleted artificial symbiosis environment allowed the diazotroph (Z78) to be expressed and provide the nitrogen sources and indole-3-acetic acid for cell growth. This study represents beneficial co-culture interaction effects of different inocula of diazotrophic bacterial cells with in vitro embryogenic calli of oil palm.

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Section: Resultsmentioning

confidence: 99%

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Lim, S.-L.,

Nabila, H. M. N.,

Atiqah, S.

et al. 2016

MJM

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“…At an early stage, this relationship allows the host plant to be better adapted to the inoculum for an effective and efficient micropropagation process (Sturz et al, 2000;Azlin et al, 2007). Some early micropropagation stages of plant-microbe interactions have also been reported, such as Rhizobium japonicum associated with tobacco and cowpea cell cultures (Scowcroft and Gibson, 1975), Azotobacter zettuovii and carrot callus (Varga et al, 1994), Azomonas insignis with strawberry callus (Preininger et al, 1997), Azospirillum sp. and Gluconacetobacter sp.…”

Section: Artificial Symbiosis Of Pgpr and In Vitro Plant Culturementioning

confidence: 99%

“…Inoculated bacteria can eventually increase the availability of nutrients and phytohormones and reduce any potential biotic and abiotic plant stresses faced by plant tissue culture material. An artificial symbiosis between selected diazotrophic PGPR and in vitro plant materials is a viable solution for transferring the inoculum to newly selected hosts ( Varga et al., 1994 ). If the cultural conditions are refined, thus, a successful symbiotic relationship between plant cells and PGPR can be created ( Maksimov et al., 2011 ).…”

Section: Artificial Symbiosis Of Pgpr and In Vitro ...mentioning

confidence: 99%

“…Some early micropropagation stages of plant-microbe interactions have also been reported, such as Rhizobium japonicum associated with tobacco and cowpea cell cultures ( Scowcroft and Gibson, 1975 ), Azotobacter zettuovii and carrot callus ( Varga et al., 1994 ), Azomonas insignis with strawberry callus ( Preininger et al., 1997 ), Azospirillum sp. and Gluconacetobacter sp.…”

Section: Artificial Symbiosis Of Pgpr and In Vitro ...mentioning

confidence: 99%

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Biotization of in vitro oil palm (Elaeis guineensis Jacq.) and its plant-microbe interactions

et al. 2023

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Continuous discovery of novel in vitro plant culture practices is always essential to promote better plant growth in the shortest possible cultivation period. An alternative approach to conventional micropropagation practice could be achieved through biotization by inoculating selected Plant Growth Promoting Rhizobacteria (PGPR) into the plant tissue culture materials (e.g., callus, embryogenic callus, and plantlets). Such biotization process often allows the selected PGPR to form a sustaining population with various stages of in vitro plant tissues. During the biotization process, plant tissue culture material imposes developmental and metabolic changes and enhances its tolerance to abiotic and biotic stresses, thereby reducing mortality in the acclimatization and pre-nursery stages. Understanding the mechanisms is, therefore crucial for gaining insights into in vitro plant-microbe interactions. Studies of biochemical activities and compound identifications are always essential to evaluate in vitro plant-microbe interactions. Given the importance of biotization in promoting in vitro plant material growth, this review aims to provide a brief overview of the in vitro oil palm plant-microbe symbiosis system.

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Arbuscular Mycorrhizae Associations and Role in Mitigation of Drought Stress in Plants

2020

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Artificial associations between Daucus and nitrogen‐fixing Azotobacter cells in vitro

Cited by 22 publications

References 11 publications

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Artificial symbiotic association and growth induction of embryogenic calli (Elaeis guineensis Jacq.) inoculated with Herbaspirillum seropedicae (Z78)

Biotization of in vitro oil palm (Elaeis guineensis Jacq.) and its plant-microbe interactions

Arbuscular Mycorrhizae Associations and Role in Mitigation of Drought Stress in Plants

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