Articular cartilage of dwarf mice: Light and electronmicroscopic studies

Silberberg, Ruth; Hasler, Mary; Silberberg, Martin

doi:10.1159/000142876

Cited by 14 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Retarded development of the epiphy seal cartilage, manifested itself even in aged dwarfs in the presence of re sidual epiphyseal cartilage in the vertebral metaphyses or diaphyses -a finding similar to that observed in the long bones [13]. With respect to their location, these cartilaginous inclusions resemble the well-known 'Schmorl nodules' of human pathology; however, the latter lesions are at tributed to rupture of the cartilaginous end-plates and subsequent hernia tion of the chondrified nucleus pulposus into the vertebral body rather than to a developmental defect.…”

Section: Discussionsupporting

confidence: 69%

Vertebral Aging in Hypopituitary Dwarf Mice

Silberberg¹

1973

Gerontology

Self Cite

View full text Add to dashboard Cite

Changes occurring in lumbar and lower thoracic vertebrae of aging dwarf mice (dwdw) were compared with those found in non-dwarfs of the same strain (Dwdw or DwDw) and of strain C₅₇B1. Dwarf vertebrae, besides remaining smaller, develop less completely and more slowly than those of non-dwarfs. Retarded development is indicated by the presence of non-resorbed epiphyseal cartilage, by diminished removal of cancellous bone from the vertebral bodies and possibly also by the failure of osseous replacement of the cartilaginous end-plates. The slowing of the aging process manifests itself in decreased degeneration of the growth zones, delayed loss of mucopolysaccharide from the matrix of the articular cartilage and by an attenuated tendency to develop spondylosis.

show abstract

Section: Discussionsupporting

confidence: 69%

Vertebral Aging in Hypopituitary Dwarf Mice

Silberberg¹

1973

Gerontology

Self Cite

View full text Add to dashboard Cite

show abstract

“…While many authors confirm the parallel alignment of fibres in the superficial zone of AC (Cameron and Robinson, 1958;McCall, 1962;Anderson, 1964;Silberberg et al, 1966;Clarke, 1971;Teshima et al, 1995), the situation in the intermediate and deep zones is more controversial. The arching of collagen fibres in the intermediate zone is described in a few papers subsequent to Benninghoffs (Clarke, 1971;Clark and Simonian, 1997) as well as suggestions that a more isotropic arrangement is present (Little et al, 1958;Hunziker, 1992).…”

Section: The Ultrastructure Of Mouse Articular Cartilage: Collagen Ormentioning

confidence: 96%

“…Mouse AC has not been studied extensively with high-resolution imaging methods except with transmission electron microscopy (TEM), (Silberberg et al, 1961;1966). Mouse AC appears to be an ideal subject for electron microscopic investigations due to the small size of the tissue, enabling the AC to be fixed whole while preserving its structural integrity as much as possible.…”

Section: The Ultrastructure Of Mouse Articular Cartilage: Collagen Ormentioning

confidence: 99%

The ultrastructure of mouse articular cartilage: Collagen orientation and implications for tissue functionality. A polarised light and scanning electron microscope study and review.

Hughes

Archer²,

Gwynn³

2005

eCM

126

120

View full text Add to dashboard Cite

Abstract.Adult mouse articular cartilage (AC) has not been thoroughly described using high resolution imaging techniques, despite the fact that the availability of knockout mice with specific extracellular matrix (ECM) mutations have renewed interest in using the mouse as a model for a variety of different human conditions. With osteoarthritis affecting millions of people worldwide, investigations into the structure and, therefore, the ability of AC to act as a load-bearing tissue, are crucial for developing treatments and prevention techniques to limit the degree of severity in this condition. Cryofixation and formaldehyde fixation as well as chemical digestion of the uncalcified regions of AC were used in combination with bright field light, polarised light and scanning electron microscopy to image the structure of adult mouse AC. Chemical digestion of the tissue revealed unique insights into the structure of mouse AC and the high cellular density of the tissue. Tightly packed sheets of collagen fibrils formed the territorial matrix (TM) of the deep zone. These were observed closely surrounding the chondrons, after applying both chemical and cryofixation techniques. The interterritorial matrix (IM), in contrast, was more isotropically arranged. The results of the study have implications for the interpretation of biomechanical functionality of mouse AC with probable applications to other species.

show abstract

“…The mature flattened chondrocytes resemble the chon-drocytes described a t the light microscopical level in the flattened cell zone in epiphyseal cartilages of fish (Haines, 1934(Haines, , 1938(Haines, , 1942Meunier, 1979) and of warm-blooded vertebrates (e.g., Howlett, 1979). The chondrocytes in Meckel's cartilage of H. bimaculatus however, seem to lack one feature which is often held to be characteristic for such cells, namely deposits of glycogen (e.g., Godman and Porter, 1960;Silberberg et al, 1964Silberberg et al, , 1966Silberberg et al, , 1976Silva and Hart, 1967;Holtrop, 1972;Silbermann and Frommer, 1974;Silbermann and Lewinson, 1978). The presence of glycogen in chondrocytes is indicative of the state of maturation of the cells and related to the synthesis of matrix polysaccharides (Knese, 1979).…”

Section: Chondrogenesk Of Meckel's Carfikgementioning

confidence: 99%

Development of cartilage and bone tissues of the anterior part of the mandible in cichlid fish: A light and TEM study

Huysseune

Sire

1992

Anat. Rec.

View full text Add to dashboard Cite

The present paper presents ultrastructural details of chondrogenesis of Meckel's cartilage and of ossification of its associated peri- and parachondral bones in a teleost fish, the cichlid Hemichromis bimaculatus. We have distinguished four stages during chondrogenesis, each of which is characterized by specific cellular and matrix features: blastema, primordium, differentiated cartilage and cartilage surrounded by perichondral bone. The blastema is characterized by prechondroblasts and the lack of cartilage matrix; the primordium by chondroblasts and the onset of secretion of matrix of fibrillar and granular nature; differentiated cartilage is characterized by chondrocytes and larger amounts of typical hyaline cartilage matrix. Once perichondral bone is laid down, the chondrocytes show degenerative features but not true hypertrophy. Differentiation of the cartilage cells is attended with cytoplasmic changes indicative of an increasing secretory activity. There is a regional calcification of the cartilage matrix by fusion of calcospherites. Chondrogenesis of the symphyseal area is continuous with that of the rami but starts slightly later. Formation of perichondral bone at the cartilage surface is attended with the deposition of a transitional zone apparently containing a mixture of the two matrices. The role of the perichondral cells is discussed and it is proposed that they may contribute to the formation of the two matrices. The transitional zone may then result either from a diffusion process or from the simultaneous deposition of elements of the two matrices. Growth of the cartilage is argued to be largely the result of matrix secretion, except in the symphyseal area where appositional growth probably occurs until the region is completely covered by perichondral bone. This paper provides a basis for further studies on the developmental interactions between cartilage, bone and teeth during mandibular development in cichlids.

show abstract

Articular cartilage of dwarf mice: Light and electronmicroscopic studies

Cited by 14 publications

References 4 publications

Vertebral Aging in Hypopituitary Dwarf Mice

Vertebral Aging in Hypopituitary Dwarf Mice

The ultrastructure of mouse articular cartilage: Collagen orientation and implications for tissue functionality. A polarised light and scanning electron microscope study and review.

Development of cartilage and bone tissues of the anterior part of the mandible in cichlid fish: A light and TEM study

Contact Info

Product

Resources

About