Arthroscopic autologous chondrocyte implantation for the treatment of a chondral defect in the tibial plateau of the knee

Ronga, Mario; Grassi, Federico Alberto; Bulgheroni, P.

doi:10.1016/j.arthro.2003.11.012

Cited by 96 publications

(76 citation statements)

References 15 publications

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“…Moreover, iron oxide labeling of chondrocytes may have immediate applicability in current tissue repair therapies such as autologous chondrocyte implantation. [39][40][41][42][43] Heymer et al 44 were the first to report on SPIO labeling specifically for the purposes of cartilage tissue engineering. Their studies showed that bone marrow-derived stem cells could still differentiate chondrogenically while retaining intracellular iron oxide, thereby permitting MRI detection within 3D collagen type I hydrogel constructs.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Ramaswamy

Greco

Uluer

et al. 2009

Tissue Engineering Part A

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Section: Discussionmentioning

confidence: 99%

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Ramaswamy

Greco

Uluer

et al. 2009

Tissue Engineering Part A

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“…In the case of autologous chondrocyte implantation (ACI), chondrocytes removed from patients are multiplied in vitro by monolayer culture to reach a minimum density of 10 million cells before they are implanted into the cartilage defect. 2 Although accumulating evidence suggest that the clinical outcome of ACI for cartilage defects is promising and has achieved up to 90% good to excellent functional improvement, [3][4][5] the biology of the chondrocytes, in particular the stability of the chondrocyte phenotype during in vitro propagation, has not been well elucidated.…”

mentioning

confidence: 99%

“…[1][2][3][4][5]20 In this study, the gene expression profiles of normal and OA chondrocytes were compared. Surprisingly, little significant difference was observed between the two groups, suggesting that the in vitro biosynthetic profile of the chondrocyte is influenced more by the culture conditions, than the disease state of the donor cartilage.…”

mentioning

confidence: 99%

Gene expression profiles of human chondrocytes during passaged monolayer cultivation

Lin

Fitzgerald

et al. 2008

Journal Orthopaedic Research

178

149

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Chondrocyte phenotype has been shown to dedifferentiate during passaged monolayer cultivation. Hence, we have investigated the expression profile of 27 chondrocyte-associated genes from both osteoarthritic cartilage tissue and healthy passaged human articular chondrocytes by quantitative real-time PCR. Our results indicate that the gene expression levels of matrix proteins and proteases in chondrocytes from monolayer culture decrease compared with those from cartilage tissue, while monolayer cultured chondrocytes from normal and osteoarthritic cartilage exhibit similar gene expression patterns. However, chondrocytic gene expression profiles were differentially altered at various stages of passage. The expression of the matrix proteins aggrecan, type II collagen, and fibromodulin inversely correlated with increasing passage number, while fibronectin and link protein exhibited a marked increase with passage. The expression of matrix proteinases MMP-3/9/13 and ADAMTS-4/5 decreased with passage, whereas proteinase inhibitors TIMP-2/3 were elevated. The cytokine IL-1 also showed increased expression with monolayer chondrocyte culture, while IGF-1 expression levels were diminished. No significant changes in TGF-b, or the chondrogenic transcription factors Sox-9, c-fos, or c-jun were observed. Our data indicates that cultured chondrocytes undergo dedifferentiation during monolayer culture, although the gene expression level of transcription factors necessary for chondrogenesis remains unchanged. This data may prove important for the future development of more specific and efficacious cultivation techniques for human articular chondrocyte-based therapies. ß

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“…Specifically, autologous chondrocyte implantation (ACI) or matrix-induced autologous chondrocyte implantation (MACI) therapies have been used for the repair of articular, chondral defects. This procedure involves enzymatically isolating and propagating autologous articular chondrocytes (obtained from the low weight bearing regions in the joint) and then implanting these cells into the defect site as a cell suspension (ACI) or in combination with a matrix material (MACI) [14,33,34]. The implanted cells produce their own extracellular matrix to repair the damage site.…”

Section: Cartilage Regenerationmentioning

confidence: 99%

Nanomaterials for cartilage tissue engineering

Chiu¹,

Waldman²

2016

Nanomaterials and Regenerative Medicine

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Arthroscopic autologous chondrocyte implantation for the treatment of a chondral defect in the tibial plateau of the knee

Cited by 96 publications

References 15 publications

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Magnetic Resonance Imaging of Chondrocytes Labeled with Superparamagnetic Iron Oxide Nanoparticles in Tissue-Engineered Cartilage

Gene expression profiles of human chondrocytes during passaged monolayer cultivation

Nanomaterials for cartilage tissue engineering

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