Arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in Brazil

André, Marcos Rogério; Denardi, Nathani Cristina Baccarim; Sousa, Keyla Carstens Marques de; Gonçalves, Luiz Ricardo; Henrique, Paloma Canedo; Ontivero, Claudia Regina Grosse Rossi; Gonzalez, Irys Hany Lima; Nery, Carolina Vaz Cabral; Chagas, Carolina Romeiro Fernandes; Monticelli, Cauê; Santis, Ana Cláudia Gabriela Alexandre de; Machado, Rosangela Zacarias

doi:10.1016/j.ttbdis.2014.03.011

Cited by 76 publications

(101 citation statements)

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“…Although A. phagocytophilum has already been detected in dogs (SANTOS et al, 2011 and brown brocket deer (SILVEIRA et al, 2014) in Brazil, previous reports also have found new genotypes of Anaplasma spp. phylogenetically similar to A. phagocytophilum in wild carnivores kept in captivity in zoos , wild birds and in cats (ANDRÉ et al, 2014) in Brazil, corroborating with the findings of the present study. Besides that, A. phagocytophilum antibodies have been found among rural workers in the northern region of the Colombia and among horses in Brazil (SALVAGNI et al, 2010), however, the agent has not yet been molecularly characterized (MÁTTAR & PARRA, 2006).…”

Section: Discussionsupporting

confidence: 92%

“…Several reports have been published about the occurrence of these agents in South America (DAGNONE et al, 2009;MACHADO et al, 2012;SACCHI et al, 2012;SALVAGNI et al, 2010;SANTOS et al, 2009SANTOS et al, , 2011SANTOS et al, , 2013SILVEIRA et al, 2014;ANDRÉ et al, 2012ANDRÉ et al, , 2014. In Colombia, although a 20% seroprevalence to A. phagocytophilum has been found among rural workers in the country's northern region, the agent has not yet been characterized molecularly in mammals (MÁTTAR & PARRA, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Anaplasma species in dogs in Colombia

Vargas-Hérnandez

André

Cendales

et al. 2016

Rev. Bras. Parasitol. Vet.

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Anaplasma platys and A. phagocytophilum are tick-borne pathogens that parasitize platelets and neutrophils, respectively, of humans and animals. The former is the etiological agent of canine cyclic thrombocytopenia, while the latter is that of canine granulocytic anaplasmosis. This work involved the detection and identification of Anaplasma species in blood samples from dogs in Colombia, using molecular techniques. Between December 2008 and April 2009, blood samples were drawn from the cephalic vein of 91 dogs in the central-western region of Colombia (cities of Bogota, Villavicencio and Bucaramanga) and stored in tubes containing EDTA. These samples were used in 16S rRNA-Anaplasma spp. nPCR and the preparation of blood smears. One (1.1%) of the 91 sampled dogs showed inclusions suggestive of Anaplasmataceae agents in the cytoplasm of platelets. Based on PCR followed by sequencing and phylogenetic analysis, A. platys and Anaplasma sp. closed related to A. phagocytophilum were detected in two and one dog, respectively. Interestingly, all the samples were negative for specific msp-2-A. phagocytophilum real-time qPCR, suggesting the circulation of an Anaplasma species phylogenetically related to A. phagocytophilum in dogs in the aforementioned region. Hence, Anaplasma spp. circulates among dogs in Colombia, albeit with low frequency. To the best of authors' knowledge, this is the first molecular detection of Anaplasma spp. in dogs in Colombia.Keywords: Dogs, Anaplasma spp., Anaplasma platys, Colombia. ResumoAnaplasma platys e A. phagocytophilum são patógenos transmitidos por carrapatos que parasitam plaquetas e neutrófilos, respectivamente, de humanos e animais. Enquanto o primeiro é agente etiológico da trombocitopenia cíclica canina, o último é o agente responsável pela anaplasmose granulocítica canina. O presente trabalho objetivou detectar e caracterizar, utiizando técnicas moleculares, a presença de espécies de Anaplasma em amostras de sague de cães na Colômbia. Entre Dezembro de 2008 e Abril de 2009, amostras de sangue colhidas em tubos contendo EDTA da veia cefálica de 91 cães da região centro-oeste da Colômbia (cidades de Bogotá, Villavicencio e Bucaramanga). As amostras de sangue foram utilizadas em reações de nPCR para 16S rRNA-Anaplasma spp. e confecção de esfregaços sanguíneos. Um (1,1%) dos 91 cães amostrados mostraram inclusões sugestivas de agentes Anaplasmataceae no citoplasma de plaquetas. Baseada na PCR seguida de sequenciamento e análise filogenética, A. platys and Anaplasma sp. relacionado a A. phagocytophilum foram detectados em dois e um cão, respectivamente. Interessantemente, todas as amostras mostraram-se negativas na PCR em tempo real quantitativa específica para msp-2-A. phagocytophilum, sugerindo a circulação de uma espécie de Anaplasma sp. filogeneticamente relacionado ao A. phagocytophilum em cães na região estudada. Embora em uma baixa freqüência, Anaplasma spp. circula entre cães na Colômbia. O presente trabalho representa a primeira detecção de Anaplasma spp. em cães na Colô...

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Molecular detection of Anaplasma species in dogs in Colombia

Vargas-Hérnandez

André

Cendales

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…and Anaplasma spp. may be present in wild carnivores , cats (BRAGA et al, 2012;ANDRÉ et al, 2014), wild birds , and wild deer (MACHADO et al, 2006;SACCHI et al, 2012). These data indicate the possibility of agents showing similar genotypes to E. chaffeensis and E. canis being involved in the positivity of sampled dogs.…”

Section: Discussionmentioning

confidence: 97%

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

Guedes

Oliveira

Carvalho

et al. 2015

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is a zoonotic disease that is caused by bacteria of the genus Ehrlichia. The aims of this study were to detect the presence of Ehrlichia spp. in the blood of dogs in Ituberá, Bahia, and to compare the sensitivities and specificities of blood smear, serological, and molecular examinations. Furthermore, this study identified factors associated with exposure to the agent in dogs in this locality. Blood samples were collected from 379 dogs and submitted for indirect immunofluorescent assay and polymerase chain reaction testing for the detection of Ehrlichia spp. antibodies and DNA, respectively. Additionally, a peripheral blood smear was obtained from the ear tip for parasite identification. Of the 379 animals, 12.4%, 32.7%, and 25.6% were identified as positive on the blood smear, serological, and molecular tests, respectively. The dogs positive in one of the three techniques were considered exposed (46.9%). Younger dogs and rural habitat were protective factors and presence of ticks and contact with other dogs were the risk factors associated with exposure to the agent. It was concluded that dogs of Ituberá have high positivity for Ehrlichia spp. and that the diagnostic methods used for detection are complementary.Keywords: Diagnosis, dog, Ehrlichia canis, Indirect Immunofluorescence, nested PCR. ResumoErliquiose é uma doença zoonótica causada por bactérias do gênero Ehrlichia. O objetivo desse estudo foi detectar a presença de Ehrlichia spp. no sangue de cães em Ituberá-BA, e comparar as sensibilidades e especificidades do esfregaço sanguíneo, e testes sorológico e molecular. Além disso, esse estudo identificou fatores associados com a exposição ao agente em cães desta localidade. Amostras de sangue foram coletadas de 379 cães e submetidas à Reação de Imunofluorescência Indireta e Reação em Cadeia de Polimerase para detecção de anticorpos e DNA de Ehrlichia spp., respectivamente. Adicionalmente, sangue periférico de ponta de orelha foi coletado para identificação do parasita. Dos 379 animais, 12,4%, 32,7% e 25,6% foram identificados como positivos no esfregaço sanguíneo, teste sorológico e molecular, respectivamente. Cães positivos em uma das três técnicas foram considerados expostos (46,9%). Cães mais novos e hábitat rural foram fatores de proteção e presença de carrapatos e contato com outros cães foram os fatores de risco associados à exposição ao agente. Foi concluído que, os cães do município de Ituberá têm alta positividade para Ehrlichia spp. e que os diferentes métodos diagnósticos utilizados para sua detecção são complementares.

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“…The other was found in domestic cats in a Brazilian zoo and may be an incidental infection transmitted from another felid carrier (André et al., 2014). …”

Section: Specificity Of Molecular Assays and The Species Conceptmentioning

confidence: 99%

A review of Theileria diagnostics and epidemiology

Mans

Pienaar

Latif

2015

International Journal for Parasitology: Parasites and Wildlife

226

219

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HighlightsSerological and molecular assays exist for most economic important Theileria species.Molecular assays are constantly being improved with regard to sensitivity and specificity.The concept of what constitute a Theileria species impacts on accurate diagnostics.Analytical specificity of molecular assays are >800 000 parasites/L blood.Parasitemia ranges may determine practical limits of detection.

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Arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in Brazil

Cited by 76 publications

References 50 publications

Molecular detection of Anaplasma species in dogs in Colombia

Molecular detection of Anaplasma species in dogs in Colombia

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

A review of Theileria diagnostics and epidemiology

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