Artesunate-quercetin/luteolin dual drug nanofacilitated synergistic treatment for malaria: A plausible approach to overcome artemisinin combination therapy resistance

Puttappa, Nethravathi; Sureshkumar, Raman; Yamjala, Karthik

doi:10.1016/j.mehy.2017.10.016

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…Thus, the advantage of a nano-delivery system in malaria treatment was based on the ability to enhance the interaction with RBCs [ 51 ]. The main goal of malaria treatment is to promote higher concentrations of drugs in intracellular parasitic vacuoles (PVs), where the parasite is hosted [ 52 ]. Here, we proposed a dual-targeted nanomimic of PEM-ARM that was capable of simultaneously targeting merozoites and infected erythrocytes in pb ANKA-infected mice for ultimate pathogen clearance.…”

Section: Discussionmentioning

confidence: 99%

Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection

Pan

et al. 2023

J Nanobiotechnol

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Background Malaria remains a serious threat to global public health. With poor efficacies of vaccines and the emergence of drug resistance, novel strategies to control malaria are urgently needed. Results We developed erythrocyte membrane-camouflaged nanoparticles loaded with artemether based on the growth characteristics of Plasmodium. The nanoparticles could capture the merozoites to inhibit them from repeatedly infecting normal erythrocytes, owing to the interactions between merozoites and heparin-like molecules on the erythrocyte membrane. Modification with a phosphatidylserine-targeting peptide (CLIPPKF) improved the drug accumulation in infected red blood cells (iRBCs) from the externalized phosphatidylserine induced by Plasmodium infection. In Plasmodium berghei ANKA strain (pbANKA)-infected C57BL/6 mice, the nanoparticles significantly attenuated Plasmodium-induced inflammation, apoptosis, and anemia. We observed reduced weight variation and prolonged survival time in pbANKA-challenged mice, and the nanoparticles showed good biocompatibility and negligible cytotoxicity. Conclusion Erythrocyte membrane-camouflaged nanoparticles loaded with artemether were shown to provide safe and effective protection against Plasmodium infection. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection

Pan

et al. 2023

J Nanobiotechnol

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“…[94][95][96] Furthermore, docking experiment in this study was motivated by the previous study which reported the anti-parasitic activity of flavonoids. 97,98 The antimalarial drug action/ mechanism can be elucidated through prediction with the binding energies of molecular interactions between plasmodium drug target proteins and chemical ligands of plant extract. Further information from the in silico study will further consolidate and provide a molecular explanation of in vivo experimental findings.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antimalarial Activity of Ethanolic Extract ofAnnona muricata L.: Anin vivoand anin silicoApproach

Nwonuma

Balogun

Gyebi

2023

J Evid Based Complementary Altern Med

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In Nigeria, Annona muricata L. has been used to treat a variety of ailments. The mechanism of the antimalarial activity of ethanolic leaf extract of Annona muricata (EEAML) was investigated using both an in vivo and an in silico approach. The experimental mice were divided into five groups: A-F. The mice in groups B-F were inoculated with Plasmodium berghei NK-65 and treated accordingly. Groups A and B are the negative and positive controls (infected and untreated), respectively. Group C received 10 mg/kg chloroquine (standard drug), whereas groups D-F received 100, 200, and 300 mg/kg body weight of the extract orally respectively. The mice were euthanized eight days after infection, and their liver and blood were collected and used in biochemical tests. Molecular docking was performed using the extract's HPLC compounds and Plasmodium falciparum proteins. In the suppressive, prophylactic, and curative tests, there was a significant decrease (p < 0.05) in parasitemia levels in groups treated with the extract compared to the positive control and standard drug. When compared to the positive control, there was a significant (p < 0.05) reduction in liver MDA, total cholesterol, and total triglyceride levels. The binding energies of luteolin and apigenin- pfprotein complexes were significantly (p < 0.05) higher compared to their respective references. The anti-plasmodial activity of the extract may result from its hypolipidemic effect, which deprives the parasite of essential lipid molecules needed for parasite growth, as well as from the inhibitory effects of apigenin and luteolin on specific proteins required for the Plasmodium metabolic pathway.

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“…Several findings suggest that plant-derived molecules exhibit antimalarial activity ( Mohanty et al, 2013 ; Saxena et al, 2018 ; Gupta et al, 2018 ). Flavonoids are the natural molecules abundantly present in fruits and vegetables, showing beneficial effects against malarial pathogenesis ( Puttappa et al, 2017 ; Amiri et al, 2018 ). Anemia and hypoglycemia are associated with severe malaria pathogenesis, which leads to mortality, especially in children and pregnant women ( Boeuf et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Dietary Ellagic Acid Against Severe Malaria Pathogenesis by Reducing Cytokine Storms and Oxidative Stress

et al. 2021

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Ellagic acid (EA), a fruit- and vegetable-derived flavonoid, has been reported for multiple pharmacological activities, which encouraged us to examine its useful effect in severe malaria pathogenesis, especially malaria-induced cytokine storms and oxidative stress linked to damage in major organs. Malaria was induced by injecting Plasmodium berghei–infected RBCs intraperitoneally into the mice. EA was given orally (5, 10, and 20 mg/kg) following Peter’s 4-day suppression test. EA exhibited the suppression of parasitemia, production of inflammatory cytokine storms and oxidative stress marker level quantified from vital organs significantly and an increase in hemoglobin, blood glucose, and mean survival time compared to the vehicle-treated infected group. EA administration also restored the blood–brain barrier integrity evidenced through Evans blue staining. Furthermore, we demonstrated the protecting effect of EA in LPS-induced inflammatory cytokine storms and oxidative stress in glial cells. The present study conclude that ellagic acid is able to alleviate severe malaria pathogenesis by reducing cytokine storms and oxidative stress–induced by malarial parasites. It also attributed promising antimalarial activity and afforded to improve the blood glucose and hemoglobin levels in treated mice. These research findings suggested the suitability of ellagic acid as a useful bioflavonoid for further study for the management of severe malaria pathogenesis.

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Artesunate-quercetin/luteolin dual drug nanofacilitated synergistic treatment for malaria: A plausible approach to overcome artemisinin combination therapy resistance

Cited by 12 publications

References 38 publications

Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection

Erythrocyte membrane with CLIPPKF as biomimetic nanodecoy traps merozoites and attaches to infected red blood cells to prevent Plasmodium infection

Evaluation of Antimalarial Activity of Ethanolic Extract ofAnnona muricata L.: Anin vivoand anin silicoApproach

Ameliorative Effects of Dietary Ellagic Acid Against Severe Malaria Pathogenesis by Reducing Cytokine Storms and Oxidative Stress

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