The pineal glands of donkey were light beige to dark brown, fusiform structure. It lies at the pineal recess caudal to the splenium of the corpus callosum and caudodorsal to the third ventricle, just in front of the rostral colliculi and in between the para hippocampal gyrus. The pineal gland was supplied from the caudal choroidal artery and branches from artery of corpus callosum. The venous drainage by the pineal veins flowing into the cerebral vein.The glial fibrillary acidic protein (GFAP) and S100 protein immunoreactivity was restricted to glial cells. They showed a heterogeneous pattern of immunostaining for (GFAP) and S100. It was conspicuous around the large pineal cyst and corpora arenacea,
Pineal gland of donkeySafwat Ebada the vicinity of the pineal cyst and corpora arenacea. The intercellular differences in the degrees of synaptophysin immunostaining may, therefore, reflect different states of a specific cellular activity. The presence of synaptophysin in pinealocytes of the normal pineal, highlights the paraneuronal nature of these cells.