Morphological and Immunohistochemical Studies on the Pineal Gland of the Donkey (Equus asinus).

Ebada, Safwat

doi:10.21608/jva.2012.44883

Cited by 7 publications

(2 citation statements)

References 47 publications

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“…Brain sand of calcium concretion was observed in the gland parenchyma between their cells and appeared as a small area of hyalinization which also found in the pineal gland of donkey described after Ebada (2012) . It was thought that these structures were increased in size and number with aging.…”

Section: Discussionmentioning

confidence: 51%

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Cytodifferentiation of pinealocytes (I and II) and astrocyte types of mature male sheep epiphysis cerebri with special emphasis on the presence of neuronal and pigmented like cells

Dahran¹,

Ghonimi²

2023

Open Vet J

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Background: The epiphysis cerebri (pineal gland) is a small-sized, photo neuroendocrine organ in the brain of most vertebrates. Their effect is through secretion of melatonin, a serotonin-derived hormone which is stimulated by darkness and inhibited by light and modulates the circadian rhythm; light and dark cycle like a biological clock, sleep patterns (sleep-wake cycle) and sexual development. Aim: This study aimed to identify and differentiate the different cell types filling the pineal gland parenchyma of mature male sheep. Methods: Pineal glands were collected and sliced parasagitally then processed histologically for light and electron microscopic examinations. Results: Two main cell types; pinealocytes and astrocytes were recognized within the gland parenchyma. Pinealocytes were the chief parenchymatus cells occupied the largest volume of the gland and were classified according to the nuclear pictures (activity status) into two subtypes; pinealocytes I (pale subtype, active) and II (dark subtype, in active). Astrocyte neuroglial cells had cytoplasmic processes which forms a huge supportive framework between the pinealocytes and clarified two types; type I were elongated cells with elongated snake shaped nucleus and type II were smaller in size, with oval nuclei. Another marginal cell type was identified as a neuron-like cell which appeared larger in size than others and distributed sporadically, has eccentric oval nucleus with prominent nucleoli and single, long cytoplasmic process that branched at its terminal forming T-shaped process looks like pseudo unipolar neuron. Moreover, aggregations of pigment granules were markedly observed in the intercellular spaces and also near the blood capillaries. With TEM a special characteristic feature of pinealocytes; synaptic ribbons were recognized that appeared as bands of electron-dense material with several synaptic spherules; vesicles adjacent to its surface helping in the multivesicular release. Conclusion: The gland parenchyma revealed two main cell types; pinealocytes and astrocytes. Each one was subdivided to two subtypes; I and II. The first one was classified according to their nuclear pictures (activity status) and the second one was according to their shape, size and cytoplasmic processes. Other cell types were also identified as neuronal and pigmented like cells in the pineal matrix.

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Section: Discussionmentioning

confidence: 51%

“…Due to their high chromatin density, they stain darkly and can be easily distinguished from pinealocytes ( Ekström and Meissl, 1997 ). And also, calcium concretions and some cysts of different size are a distinct feature in the pineal gland ( Ebada, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Cytodifferentiation of pinealocytes (I and II) and astrocyte types of mature male sheep epiphysis cerebri with special emphasis on the presence of neuronal and pigmented like cells

Dahran¹,

Ghonimi²

2023

Open Vet J

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A Seasonal and Age‐Related Study of Interstitial Cells in the Pineal Gland of Male Viscacha (Lagostomus maximus maximus)

Busolini

Rosales

Filippa

et al. 2017

The Anatomical Record

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The pineal gland of viscacha exhibits histophysiological variations throughout the year, with periods of maximal activity in winter and minimal activity in summer. The aim of this work is to analyze the interstitial cells (IC) in the pineal gland of male viscachas in relation to season and age. The S-100 protein, glio-fibrillary acidic protein (GFAP), and vimentin were detected in adult and immature animals by immunohistochemistry (IHC). Double-IHC was also performed. The S-100 protein was localized within both, IC nucleus and cytoplasm. GFAP was present only in the cytoplasm. Vimentin was expressed in some IC, besides endothelial cells, and perivascular spaces. In the adult males, the morphometric parameters analyzed for the S-100 protein and GFAP exhibited seasonal variations with higher values of immunopositive area percentage in winter and lower values in summer, whereas the immature ones showed the lowest values for all the adult animals studied. Colocalization of S-100 protein and GFAP was observed. The IC exhibited differential expression for the proteins studied, supporting the hypothesis of the neuroectodermal origin. The IC generate an intraglandular communication network, suggesting its participation in the glandular activity regulation processes. The results of double-IHC might indicate the presence of IC in different functional stages, probably related to the needs of the cellular microenvironment. The morphometric variations in the proteins analyzed between immature and adult viscachas probed to be more salient in the latter, suggesting a direct relationship between the expression of the S-100 protein and GFAP, and animal age. Anat Rec, 2017. © 2017 Wiley Periodicals Inc. Anat Rec, 300:1847-1857, 2017. © 2017 Wiley Periodicals, Inc.

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Synchronization of the Pineal and Pituitary Morphophysiology by Photoperiodic Environmental Signals

Filippa,

Busolini,

Mohamed

2024

Plains Vizcachas

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Morphological and Immunohistochemical Studies on the Pineal Gland of the Donkey (Equus asinus).

Cited by 7 publications

References 47 publications

Cytodifferentiation of pinealocytes (I and II) and astrocyte types of mature male sheep epiphysis cerebri with special emphasis on the presence of neuronal and pigmented like cells

Cytodifferentiation of pinealocytes (I and II) and astrocyte types of mature male sheep epiphysis cerebri with special emphasis on the presence of neuronal and pigmented like cells

A Seasonal and Age‐Related Study of Interstitial Cells in the Pineal Gland of Male Viscacha (Lagostomus maximus maximus)

Synchronization of the Pineal and Pituitary Morphophysiology by Photoperiodic Environmental Signals

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