Artemisinin Resistance in Cambodia: A Clinical Trial Designed to Address an Emerging Problem in Southeast Asia

Noedl, Harald; Se, Youry; Sriwichai, Sabaithip; Schaecher, Kurt E.; Teja‐Isavadharm, Paktiya; Smith, Bryan; Rutvisuttinunt, Wiriya; Bethell, Delia; Surasri, Sittidech; Fukuda, Mark M.; Socheat, Duong; Thap, Lon Chan

doi:10.1086/657120

Cited by 171 publications

(138 citation statements)

References 30 publications

Supporting

Mentioning

127

Contrasting

Unclassified

Order By: Relevance

“…In recent years, evidence of confirmed emerging resistance to artemisinins has been gathered from clinical studies in western Cambodia as well as the bordering areas between Thailand and Myanmar [27][28][29][30] . Furthermore, the observation of pfmdr1 wild type and increasing of gene copy number may suggest declining of artesunate-mefloquine treatment efficacy in P. falciparum isolates in this border area.…”

Section: Discussionmentioning

confidence: 99%

Polymorphic patterns of pfcrt and pfmdr1 in Plasmodium falciparum isolates along the Thai-Myanmar border

Muhamad

Chai่jaroenkul

Phompradit

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Polymorphic patterns of pfcrt and pfmdr1 in Plasmodium falciparum isolates along the Thai-Myanmar border

Muhamad

Chai่jaroenkul

Phompradit

et al. 2013

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

“…These differences may be related to different sampling times, different pharmacokinetic models, population variations in drug handling, and regional differences in susceptibilities in P. falciparum isolates to artemisinins and the partner drugs 36 in a region where recent data suggest that artemisinin resistance has developed. [40][41][42][43] It would seem that enrolment parasitemia significantly influenced the parasite clearance times. Thus, high parasitemias were associated with a longer parasite clearance times.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Efficacies of Artemisinin-Based Combination Therapies in Nigerian Children with Uncomplicated Falciparum Malaria during Five Years of Adoption as First-Line Treatments

Gbotosho

Sowunmi²,

Happi³

et al. 2011

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The therapeutic efficacies of 3-day regimens of artesunate-amodiaquine and artemether-lumefantrine during 5 years of adoption as first-line treatments were evaluated in 811 ≤ 12-year-old malarious children. Compared with artemether-lumefantrine, amodiaquine-artesunate significantly reduced the proportion of children with fever and parasitemia 1 day after treatment (day 1; P < 0.008 for both). The proportion of parasitemic children on day 2 and gametocytemia on presentation and carriage reduced significantly over the years ( P < 0.000001 and P < 0.03, respectively; test for trend). Overall efficacy was 96.5% (95% confidence interval [CI] = 94.5-98.6) and remained unchanged over the years ( P = 0.87; test for trend). Kinetics of parasitemias after treatments were estimated by a non-compartmental model. Declines of parasitemias were monoexponential, with a mean elimination half-life of 1.09 hours (95% CI = 1.0-1.16). Parasitemia half-lives and efficacy were similar for both regimens and in all ages. Artesunate-amodiaquine and artemether-lumefantrine remain efficacious treatments of uncomplicated falciparum malaria in Nigerian children 5 years after adoption.

show abstract

“…The efficacy and effectiveness of AL as an antimalarial drug has been reported (Falade et al, 2005;Yeka et al 2008;Zurovac et al, 2008). Recent reports from Africa have shown some evidence of clinical and parasitological failure after treatment with AL (Yang et al, 2003;Jambou et al, 2005;Noedl et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Pfmdr 1 N86Y and Y184F Alleles is Associated with Recurrent Parasitemia following Treatment of Uncomplicated Malaria with Artemether-Lumefantrine in Nigerian Patients

Emilia¹,

Victoria²,

Christian³

et al. 2016

J App Pharm Sci

View full text Add to dashboard Cite

We investigated and compared genetic variations in Plasmodium falciparum multidrug resistance 1 gene (Pfmdr 1) in patients showing good therapeutic response (GTR) and artemisinin resistance (AR) following artemetherlumefantrine (AL) treatment of uncomplicated malaria in Nigeria. Some 150 malaria patients were subjected to AL treatment and therapeutic efficacy was monitored for 28 days. Parasite genomic DNA was isolated followed by nested polymerase chain reaction (PCR). Genotyping of Pfmdr 1 gene for specific genetic variants: N86Y, Y184F, S1034C and N1042D were done using PCR-restriction fragment length polymorphism (PCR-RFLP).Out of 121 patients that were P. falciparum positive, 46 % (56) and 54 % (65) showed good therapeutic response and artemisinin resistance respectively, with 5.4 % and 98.3 % being mutated in the GTR and AR group respectively. The most prevalent mutations were Y184F (44.1 %) and N86Y (40.7 %). There was significant increase (p<0.001) in the prevalence of Pfmdr 1 mutation in the post treatment compared to the pretreatment group.Prevalence of Pfmdr1 86Y and 184F alleles is associated with artemisinin resistance and presence of AL drug significantly induced genetic variation in the plasmodial gene.

show abstract

Artemisinin Resistance in Cambodia: A Clinical Trial Designed to Address an Emerging Problem in Southeast Asia

Abstract: ClinicalTrials.gov identifier NCT00479206.

Cited by 171 publications

References 30 publications

Polymorphic patterns of pfcrt and pfmdr1 in Plasmodium falciparum isolates along the Thai-Myanmar border

Polymorphic patterns of pfcrt and pfmdr1 in Plasmodium falciparum isolates along the Thai-Myanmar border

Therapeutic Efficacies of Artemisinin-Based Combination Therapies in Nigerian Children with Uncomplicated Falciparum Malaria during Five Years of Adoption as First-Line Treatments

Prevalence of Pfmdr 1 N86Y and Y184F Alleles is Associated with Recurrent Parasitemia following Treatment of Uncomplicated Malaria with Artemether-Lumefantrine in Nigerian Patients

Contact Info

Product

Resources

About