Artemisinin conferred ERK mediated neuroprotection to PC12 cells and cortical neurons exposed to sodium nitroprusside-induced oxidative insult

Zheng, Wenhua; Chong, Cheong-Meng; Wang, Haitao; Zhou, Xin; Zhang, Lang; Wang, Rikang; Meng, Qingwei; Lazarovici, Philip; Fang, Jiankang

doi:10.1016/j.freeradbiomed.2016.05.023

Cited by 66 publications

(61 citation statements)

References 61 publications

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“…Beside these effects, previous studies including some from our group, have shown that artemisinin and its derivatives have neuroprotective effects suggesting that artemisinin might be potential candidate for AD therapy [16][17][18][19]. It has been recognized that artemisinin-mediated neuroprotection against neuronal apoptosis from various insults is regulated by ERK1/2 survival/apoptotic signaling pathway rather than the Akt pathway [16,18,19]. In addition, researchers also found that artemisinin was able to stimulate the p38MAPK pathway [16,20].…”

Section: Introductionmentioning

confidence: 91%

“…In clinical relevance, artemisinin is among the most effective therapies for malaria with great safety and the preferred first-line treatment for the disease [15]. Beside these effects, previous studies including some from our group, have shown that artemisinin and its derivatives have neuroprotective effects suggesting that artemisinin might be potential candidate for AD therapy [16][17][18][19]. It has been recognized that artemisinin-mediated neuroprotection against neuronal apoptosis from various insults is regulated by ERK1/2 survival/apoptotic signaling pathway rather than the Akt pathway [16,18,19].…”

Section: Introductionmentioning

confidence: 95%

“…The cell viability was measured by the MTT assay as previously described [19,25]. Briefly, PC12 cells were seeded in 96-well plates at a density of 5 × 10 3 cells/well.…”

Section: Mtt Assaymentioning

confidence: 99%

“…Furthermore, our group have recently reported that artemisinin can successful protect PC12 and primary neurons from β-amyloid insult [18]. Different studies have shown that artemisinin inhibited superoxide anions and had radical scavenging activity against the highly reactive hydroxyl radicals, while the understanding of mechanisms that participated in its antioxidant effects is still very limited [19,21]. PC12 cell line with most of the typical features of primary neuron, originally derived from rat pheochromocytoma, is a useful neuronal model for the study of AD as well as other neurodegenerative disease, and was also widely used to investigate free ROS biochemical pathways related to cell apoptosis and neuroprotection [18,22,23].…”

Section: Introductionmentioning

confidence: 99%

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The p38-MAPK Pathway Is Involved in Neuroprotection of Artemisinin against H2O2-induced Apoptosis in PC12 Cells

Liu¹,

Zeng²,

Gaur³

et al. 2017

JPP

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Oxidative stress, owing to the excessive production of ROS (reactive oxygen species), is one of the leading causes for the progression of AD (Alzheimer's disease). Increasing evidences suggested that oxidative stress insult impaired the physiological functioning of neuronal cells by inducing cell apoptosis. The search for drug candidates that can effectively protect neurons from oxidative stress insult might hold therapeutic potential for AD. In the present study, we tested the neuroprotective effects and the related action mechanisms of artemisinin, a FDA-approved anti-malarial drug, against H 2 O 2 induced oxidative damage in PC12 cells. It was found that artemisinin reduced cell viability loss caused by hydrogen peroxide (H 2 O 2 ) in PC12 cells. In addition, data from Flow cytometry displayed that artemisinin significantly decreased the apoptosis of PC12 cells induced by H 2 O 2 . Furthermore Western blot analysis displayed that artemisinin stimulated the p38MAPK signaling, while treatment of PC12 cells with specific p38MAPK pathway inhibitor SB203580 blocked the neuroprotective effect of artemisinin. These results together indicated that artemisinin is a potential protectant, and it protects PC12 cells against H 2 O 2 injury through activation of the p38MAPK pathway.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 95%

“…The cell viability was measured by the MTT assay as previously described [19,25]. Briefly, PC12 cells were seeded in 96-well plates at a density of 5 × 10 3 cells/well.…”

Section: Mtt Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The p38-MAPK Pathway Is Involved in Neuroprotection of Artemisinin against H2O2-induced Apoptosis in PC12 Cells

Liu¹,

Zeng²,

Gaur³

et al. 2017

JPP

View full text Add to dashboard Cite

show abstract

“…Mitochondrial activity, a measure of cell death, was measured by the MTT assay as previously described [24]. Briefly, PC12 cells were seeded in 96-well plates at a density of ×10 3 cells/well.…”

Section: Mtt Assaymentioning

confidence: 99%

Berberine Protects against Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells through Activation of ERK1/2 Pathway

Liu¹,

Zeng²,

Gaur³

et al. 2017

Clin Exp Pharmacol

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Oxidative stress is a much-recognized phenomenon linked with the progression of Neurodegenerative Diseases (NDs) due to imbalances in redox homeostasis. Increasing evidence indicates that excessive Reactive Oxygen Species (ROS), impairing the physiological functions of neurons via inducing cell apoptosis, is the main cause of NDs. The drug candidates are required that can effectively protect neurons from oxidative stress insult to slow down the process of neurodegenerative diseases. In present study, we investigated the protective effect and the underlying mechanisms of berberine (BBR, an isoquinoline alkaloid isolated from the herb Rhizoma coptidis, against oxidative damage in PC12 cells. It was found that BBR was able to suppress hydrogen peroxide (H 2 O 2 )-induced cell death in PC12 cells. Flow cytometry revealed that BBR significantly reduced the apoptosis of PC12 cells exposed to H 2 O 2 . Western blot analysis displayed that BBR stimulated the extracellular regulated ERK1/2 survival signaling, while application of PC12 cells with ERK1/2 pathway inhibitor PD98059 blocked the neuroprotective effect of BBR. These results together indicated that BBR is a potential protectant, and it protects PC12 cells against H 2 O 2 toxicity through activated the ERK1/2 pathway.

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A Gene‐Switch Platform Interfacing with Reactive Oxygen Species Enables Transcription Fine‐Tuning by Soluble and Volatile Pharmacologics and Food Additives

Huang,

Xue,

Teixeira

et al. 2024

Advanced Science

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Synthetic biology aims to engineer transgene switches for precise therapeutic protein control in cell‐based gene therapies. However, off‐the‐shelf trigger‐inducible gene circuits are usually switched on by single or structurally similar molecules. This study presents a mammalian gene‐switch platform that controls therapeutic gene expression by a wide range of molecules generating low, non‐toxic levels of reactive oxygen species (ROS). In this system, KEAP1 (Kelch‐like ECH‐associated protein 1) serves as ROS sensor, regulating the translocation of NRF2 (nuclear factor erythroid 2‐related factor 2) to the nucleus, where NRF2 binds to antioxidant response elements (ARE) to activate the expression of a gene of interest. It is found that a promoter containing eight‐tandem ARE repeats is highly sensitive to the low ROS levels generated by the soluble and volatile molecules, which include food preservatives, food additives, pharmaceuticals, and signal transduction inducers. In a proof‐of‐concept study, it is shown that many of these compounds can independently trigger microencapsulated engineered cells to produce sufficient insulin to restore normoglycemia in experimental type‐1 diabetic mice. It is believed that this system greatly extends the variety of small‐molecule inducers available to drive therapeutic gene switches.

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Artemisinin conferred ERK mediated neuroprotection to PC12 cells and cortical neurons exposed to sodium nitroprusside-induced oxidative insult

Cited by 66 publications

References 61 publications

The p38-MAPK Pathway Is Involved in Neuroprotection of Artemisinin against H2O2-induced Apoptosis in PC12 Cells

The p38-MAPK Pathway Is Involved in Neuroprotection of Artemisinin against H2O2-induced Apoptosis in PC12 Cells

Berberine Protects against Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells through Activation of ERK1/2 Pathway

A Gene‐Switch Platform Interfacing with Reactive Oxygen Species Enables Transcription Fine‐Tuning by Soluble and Volatile Pharmacologics and Food Additives

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