A Gene‐Switch Platform Interfacing with Reactive Oxygen Species Enables Transcription Fine‐Tuning by Soluble and Volatile Pharmacologics and Food Additives
Jinbo Huang,
Shuai Xue,
Ana Palma Teixeira
et al.
Abstract:Synthetic biology aims to engineer transgene switches for precise therapeutic protein control in cell‐based gene therapies. However, off‐the‐shelf trigger‐inducible gene circuits are usually switched on by single or structurally similar molecules. This study presents a mammalian gene‐switch platform that controls therapeutic gene expression by a wide range of molecules generating low, non‐toxic levels of reactive oxygen species (ROS). In this system, KEAP1 (Kelch‐like ECH‐associated protein 1) serves as ROS se… Show more
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