Arsenite oxidation in batch reactors with alginate‐immobilized ULPAs1 strain

Simeonova, Diliana D.; Micheva, Kalina; Müller, Daniel; Lagarde, Florence; Lett, Marie‐Claire; Groudeva, V.; Lièvremont, Didier

doi:10.1002/bit.20530

Cited by 45 publications

(22 citation statements)

References 23 publications

(18 reference statements)

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“…This physiologically diverse grouping includes both heterotrophic and chemolithoautotrophic arsenite oxidizers and is consistent with the hypothesis that arsenic species may have played a crucial role in the early stages in the development of life prior to the cyanobacterial evolution and formation of significant free oxygen levels (Lebrun et al 2003). Bacterial oxidation of arsenite to arsenate represents a potential partial detoxification mechanism and has applications in bioremediation (Simeonova et al 2005) because it generates the less toxic and less mobile form of arsenic. Indeed, potentially suitable arsenite (As III)-detoxification bacteria have been suggested (Oremland and Stolz 2003).…”

Section: Introductionsupporting

confidence: 84%

Isolation and characterization of arsenite-oxidizing bacteria from arsenic-contaminated soils in Thailand

Kinegam

Yingprasertchai

Tanasupawat

et al. 2008

World J Microbiol Biotechnol

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Two hundred and eighty-eight arsenic-resistant bacteria were isolated by an enrichment culture method from a total of 69 arsenic-contaminated soil-samples collected from Dantchaeng district in Suphanburi province (47 samples), and from Ron Phiboon district in Nakhon Sri Thammarat province (22 samples), in Central and Southern Thailand, respectively. Twenty-four of the 288 isolated arsenic-resistant bacteria were found to be arsenite-oxidizing bacteria. On the basis of their morphological, cultural, physiological, biochemical and chemotaxonomic characteristics, and supported by phylogenetic analysis based upon their 16S rRNA gene sequences, they were divided into five groups, within the genera Acinetobacter, Flavobacterium, Pseudomonas, Sinorhizobium and Sphingomonas, respectively. Within genera, phylogenetic analysis using the 16S rRNA gene sequences suggested that they were comprised of at least ten species, five isolates being closely related to known bacteria (Acinetobacter calcoaceticus NCCB 22016 T , Pseudomonas plecoglossicida FPC951 T , Ps. knackmussii B13 T , Sinorhizobium morelense Lc04 T , and Sphingomonas subterranea IFO16086 T ). The other five proposed species are likely to be new species closely related to Flavobacterium johnsoniae, Sinorhizobium morelense, Acinetobacter calcoaceticus and Pseudomonas plecoglossicida, but this awaits further characterization for confirmation of the taxonomic status. No overlap in isolated species or strains was observed between the two sites. The strain distribution and characterization are described.

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Section: Introductionsupporting

confidence: 84%

Isolation and characterization of arsenite-oxidizing bacteria from arsenic-contaminated soils in Thailand

Kinegam

Yingprasertchai

Tanasupawat

et al. 2008

World J Microbiol Biotechnol

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“…For some of them, biogeochemical function in the environment has been described (Connon et al, 2008;Drewniak et al, 2010) and/or a practical application in bioremediation has been proposed (e.g. Simeonova et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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Plasmid pSinA of Sinorhizobium sp. M14 (Alphaproteobacteria) is the first described, natural, self-transferable plasmid harboring a complete set of genes for oxidation of arsenite. Removal of this plasmid from cells of the host strain caused the loss of resistance to arsenic and heavy metals (Cd, Co, Zn and Hg) and abolished the ability to grow on minimal salt medium supplemented with sodium arsenite as the sole energy source. Plasmid pSinA was introduced into other representatives of Alphaproteobacteria which resulted in acquisition of new abilities concerning arsenic resistance and oxidation, as well as heavy metals resistance. Microcosm experiments revealed that plasmid pSinA can also be transferred via conjugation into other indigenous bacteria from microbial community of As-contaminated soils, including representatives of Alpha-and Gammaproteobacteria. Analysis of "natural" transconjugants showed that pSinA is functional (expresses arsenite oxidase) and is stably maintained in their cells after approximately 60 generations of growth under nonselective conditions. This work clearly demonstrates that pSinA is a self-transferable, broad-host-range plasmid, which plays an important role in horizontal transfer of arsenic metabolism genes.

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“…2H3AsO3 + O2 → HAsO4 2-+ H2AsO4 -+ 3H + Nevertheless most remediation studies focused on using heterotrophs which requires organic substrate for their growth (Simeonova et al, 2005). Recently several chemolithotrophic oxidizers that are able to fix CO2 coupled to the oxidation of As[III] to As [V] were reported mainly on their ecology, phylogenetic relationship, and physiological characteristics (Rhine et al, 2005;Rhine et al, 2007;Garcia-Dominguez et al, 2008).…”

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confidence: 99%

Arsenite oxidation by a facultative chemolithotrophic bacterium SDB1 isolated from mine tailing

Lugtu

Choi

2009

J Microbiol.

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An arsenite (As[III])-oxidizing bacterium, SDB1, was isolated from mine tailing collected from the Sangdong mine area in Korea and showed chemolithotrophic growth on As[III] and CO(2) as the respective electron and carbon sources. SDB1 is Gram-negative, rod-shaped, and belongs to the Sinorhizobium-Ensifer branch of alpha-Proteobacteria. Growth and As[III] oxidation was enhanced significantly by the presence of yeast extract (0.005%) in minimal salt medium containing 5 mM As[III]; decreasing the doubling time from 9.8 to 2.1 h and increasing the As [III] oxidation rate from 0.014 to 0.349 pmol As [III] oxidized cell(-1) h(-1). As[III] oxidation nearly stopped at pH around 4 and should be performed at pH 7 approximately 8 to be most effective. SDB1 was immobilized in calcium-alginate beads and the oxidation capacity was investigated. Specific As[III] oxidation rates obtained with SDB1 (10.1-33.7 mM As[III] oxidized g(-1) dry cell h(-1)) were 10 approximately 16-times higher than those reported previously with a heterotrophic bacterial strain (Simeonova et al., 2005). The stability and reusability of immobilized SDB1 strongly suggested that the immobilized SDB1 cell System can make the As[III] oxidation process technically and economically feasible in practical applications.

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Arsenite oxidation in batch reactors with alginate‐immobilized ULPAs1 strain

Cited by 45 publications

References 23 publications

Isolation and characterization of arsenite-oxidizing bacteria from arsenic-contaminated soils in Thailand

Isolation and characterization of arsenite-oxidizing bacteria from arsenic-contaminated soils in Thailand

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Arsenite oxidation by a facultative chemolithotrophic bacterium SDB1 isolated from mine tailing

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