Arsenicicoccus dermatophilus sp. nov., a hypha-forming bacterium isolated from the skin of greater flamingos (Phoenicopterus roseus) with pododermatitis

Abstract

Arsenicicoccus dermatophilus sp. nov., a hypha-forming bacterium isolated from the skin of greater flamingos (Phoenicopterus roseus) with pododermatitis Dermatophilus-like bacteria were observed in histological examinations of samples of diseased foot skin from greater flamingos (Phoenicopterus roseus) living in zoological gardens in Switzerland. When grown on TSA-SB containing polymyxin B, the bacteria isolated from these skin samples formed hyphae, as is typical for Dermatophilus congolensis, but these bacteria were non-haemolytic. The closest relatives based on 16S rRNA gene sequences were the two members of the genus Arsenicicoccus, Arsenicicoccus bolidensis and Arsenicicoccus piscis. A representative of the isolated strains shared 34.3 % DNA-DNA relatedness with the type strain of A. bolidensis, 32.3 % with the type strain of A. piscis and 34.5 % with the type strain of D. congolensis, demonstrating that these strains do not belong to any of these species. The phenotypic characteristics differed from those of members of the genus Arsenicicoccus as well as from those of D. congolensis. The G+C content of strain KM 894/11 T was 71.6 mol%. The most abundant fatty acids were iso-C 15 : 0 , summed feature 3 (including C 16 : 1 v7c and/or iso-C 15 : 0 2-OH) and C 18 : 1 v9c. MK-8(H4) was the predominant menaquinone. Cell-wall structure analysis revealed that the peptidoglycan type was A3c LL-Dpm-Gly (type A41.1). Based on genotypic and chemotaxonomic characteristics, the isolated strains represent a novel species within the genus Arsenicicoccus, for which the name Arsenicicoccus dermatophilus sp. nov. is proposed. The type strain is KM 894/11 T (5DSM 25571 T 5CCUG 62181 T 5CCOS 690 T ), and strain KM 1/12 (5DSM 255725CCUG 621825CCOS 691) is a reference strain.

Pododermatitis in Captive and Free-Ranging Greater Flamingos (Phoenicopterus roseus)

“…However, the bacterium A. dermatophilus was isolated from skin scrapings of all 20 chicks in 2011 at 12 weeks of age in the course of a bacteriologic study. 10 None had macroscopic foot lesions at that time point. These chicks grew up in an enclosure with water ponds filled with fine-granular sand, 42 while in 2010, the same enclosure had no sand cover on the concrete flooring.…”

“…Bacteria were isolated from skin scrapings of several flamingos in the group, identified as Arsenicicoccus dermatophilus, a novel Micrococcus-like bacterium, similar to Dermatophilus sp. 10 The aim of this study was to investigate the histologic lesions of flamingos affected and unaffected with macroscopic pododermatitis using samples from captive and free-ranging birds. Molecular biologic tests for viruses, immunohistochemistry, and transmission electron microscopy were performed on individual samples to identify infectious agents.…”

Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus

“…S1, available in the online Supplementary Material). The polar lipid patterns of strain KIS22-12 T and Oryzihumus leptocrescens KACC 15268 T were differentiated from the closely related members of the family Intrasporangiaceae in that the genera Ornithinicoccus and Ornithinimicrobium contained phosphatidylglycerol and the genus Arsenicicoccus did not have DPG and PI (Gobeli et al , 2013; Groth et al , 1999, 2001). The peptidoglycan of strain KIS22-12 T contained alanine, glutamic acid and 2,6-diaminopimelic acid (A 2 pm) at a molar ratio of 1.8 : 1.0 : 0.4.…”

A Novel Species of the Genus Arsenicicoccus Isolated From Human Blood Using Whole-Genome Sequencing

“…The genus Arsenicicoccus comprises gram-positive, facultatively anaerobic, catalase-positive, and non-spore-forming bacteria that have been isolated from environmental sources and animal specimens [1][2][3]. Arsenicicoccus was first proposed in 2004 by Collins, et al [1], who described Arsenicicoccus bolidensis isolated from mine sediment [4].…”

“…Arsenicicoccus is a member of the family Intrasporangiaceae and initially comprised a single species, A. bolidensis. Subsequently, two additional Arsenicicoccus species have been reported, A. piscis and A. dermatophilus, from the intestinal tract of the Japanese fish (Sillago japonica) and flamingo foot skin, respectively [2,3].…”