Arsenic toxicity in the water weed Wolffia arrhiza measured using Pulse Amplitude Modulation Fluorometry (PAM) measurements of photosynthesis

Ritchie, Raymond J.; Mekjinda, Nutsara

doi:10.1016/j.ecoenv.2016.06.004

Cited by 14 publications

(10 citation statements)

References 40 publications

(54 reference statements)

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“…HMs impact on this basic process may be also shown in decrease of photosynthetic pigment content and/or chlorophyll a / b ratio due to the inhibition of enzymes involved in chlorophyll biosynthesis as well as disorganization of organelle ultrastructure (Gill et al 2012; Kumar and Prasad 2018). Therefore, the analysis of photosynthetic pigment content, chlorophyll a fluorescence, and chloroplast ultrastructure allows the comprehensive assessment of plant physiological status under stress conditions (Ritchie and Mekjinda 2016; Muszyńska et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of heavy metal-induced responses in Silene vulgaris ecotypes

et al. 2019

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Silene vulgaris is a pseudometallophyte that spontaneously occurs in various ecological niches. Therefore, three ecotypes of this species representing calamine (CAL), serpentine (SER), and non-metallicolous (NM) populations were investigated in this study. Owing to the presence of Pb or Ni ions in natural habitats from metallicolous populations originated, we used these metals as model stressors to determine the survival strategy of tested ecotypes and analyze metal distribution at various levels of organism organization. We focused on growth tolerance, non-enzymatic antioxidants, and photosynthetic apparatus efficiency as well as anatomical and ultrastructural changes occurred in contrasting ecotypes exposed in vitro to excess amounts of Pb 2+ and Ni 2+ . Although Ni application contributed to shoot culture death, the study revealed that the mechanisms of Pb detoxification differed between ecotypes. The unspecific reaction of both metallicolous specimens relied on the formation of effective mechanical barrier against toxic ion penetration, while the Pb appearance in the protoplasts led to the activation of ecotype-specific intracellular defense mechanisms. Hence, the response of CAL and SER ecotypes was almost unchanged under Pb treatment, whereas the reaction of NM one resulted in growth disturbances and physiological alternations. Moreover, both metallicolous ecotypes exhibited increase generation of reactive oxygen species (ROS) in leaves, even before the harmful ions got into these parts of plants. It may implicate the potential role of ROS in CAL and SER adaptation to heavy metals and, for the first time, indicate on integral function of ROS as signaling molecules in metal-tolerant species.

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Section: Introductionmentioning

confidence: 99%

Evaluation of heavy metal-induced responses in Silene vulgaris ecotypes

et al. 2019

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“…Our absorptance values for N. aquatica are shown in Table 1B. As found here, experimentally measured absorptance values for blue light (Abt 465nm ) are typically found to be substantially different to the default value (in various aquatic plants [5,61] , Nymphaea caerulea [62] and Wolffia arrhiza [48] .…”

Section: Absorptance Measurements Using the Reflectance-absorptance-t...mentioning

confidence: 61%

“…It is found experimentally that if fluorescence yield (Y) is plotted against irradiance (E) it follows a simple exponential decay function of the form y = e -kx [18,[39][40][41][42]48,49,63,64] . The WinControl software calculates relative ETR (rETR) based on a default leaf absorptance factor (Abt F = 0.84).…”

Section: Calculation Of Photosynthetic Electron Transport Rates and O...mentioning

confidence: 99%

“…Non-Photochemical Quenching is related to the magnitude of the Proton Motive Force (PMF) that exists across the thylakoids in chloroplasts and the loss of absorbed energy as waste heat [37,[44][45][46][47] . The high variability of NPQ and poorly fitting kinetics makes it less useful than is sometimes supposed as a measure of photosynthetic stress [18,40,41,45,48,49] .…”

Section: Introductionmentioning

confidence: 99%

“…PAM fluorometers can perform measurements of the light reactions of photosynthesis very quickly [18,[35][36][37][38][39]42,48,50,51] and measure the light reactions directly and are not limited by O 2 and CO 2 diffusion problems [18,40,41] . The air ventilation system makes attempts to estimate photosynthesis and respiration rates in Nymphoid plants based on gas exchange using an oxygen electrode or an IRGA problematic because of difficulties in identifying the pool of gas being used as a source of CO 2 .…”

Section: Introductionmentioning

confidence: 99%

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Photosynthesis of Submerged and Surface Leaves of the Dwarf Water Lily (Nymphoides aquatica) Using PAM Fluorometry

Ritchie

2022

j. of bot. res.

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Dwarf Water Lilies (Nymphoides aquatica (J.F. Gmel) Kuntze have floating and submerged leaves. Some submerged aquatic vascular plants have a form of CAM (Crassulacean Acid Metabolism) called Submerged Aquatic Macrophyte (SAM) metabolism. Blue-diode based PAM technology was used to measure the Photosynthetic Oxygen Evolution Rate (POER: 1O2 ≡ 4e– ). Optimum Irradiance (Eopt), maximum POER (POERmax) and quantum efficiency (α0) all vary on a diurnal cycle. The shape of the POER vs. E curves is different in seedling, submerged and surface leaves. Both Eopt and POERmax are very low in seedling leaves (Eopt ≈ 104 μmol photon m–2 s–1, PPFD; POERmax ≈ 4.95 µmol O2 g–1 Chl a s–1), intermediate in mature submerged leaves (Eopt ≈ 419 µmol photon m–2 s–1 PPFD, POERmax ≈ 38.1 µmol O2 g–1 Chl a s–1) and very high in surface leaves (Eopt ≈ 923 µmol photon m–2 s–1 PPFD, POERmax ≈ 76.1 µmol O2 g–1 Chl a s–1). Leaf titratable acid (C4 acid pool) is too small (≈20 to 50 mol H+ m–3) to support substantial SAM metabolism. Gross daily photosynthesis of surface leaves is ≈3.71 g C m–2 d–1 in full sun and as much as 1.4 gC m–2 d–1 in shaded submerged leaves. There is midday inhibition of photosynthesis.

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Duckweed: a potential phytosensor for heavy metals

Sharma

Lenaghan

2022

Plant Cell Rep

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Arsenic toxicity in the water weed Wolffia arrhiza measured using Pulse Amplitude Modulation Fluorometry (PAM) measurements of photosynthesis

Cited by 14 publications

References 40 publications

Evaluation of heavy metal-induced responses in Silene vulgaris ecotypes

Evaluation of heavy metal-induced responses in Silene vulgaris ecotypes

Photosynthesis of Submerged and Surface Leaves of the Dwarf Water Lily (Nymphoides aquatica) Using PAM Fluorometry

Duckweed: a potential phytosensor for heavy metals

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