During the period 1990-2002 in Himachal Pradesh, India, 103 new patients with cutaneous sporotrichosis were detected. Lymphocutaneous and fixed cutaneous varieties were the most common and seen in 49% and 43% respectively. Chronic non-specific inflammation without granuloma formation was seen in 46% skin biopsy specimens, 29% showed acute or chronic inflammation and tuberculoid granuloma formation was seen in 25%. In 32% of cases Sporothrix schenckii growth was seen on Sabouraud glucose agar. A saturated solution of potassium iodide was used as first-line treatment and in 93% patients healing of lesions occurred in 4-32 weeks (average 8.7 weeks) without significant side-effects. Itraconazole, used in 12 patients, was highly effective in recommended doses. Rapid healing was seen with higher than previously used doses of fluconazole in one patient who also had unusual exacerbation of lesional inflammation with saturated solution of potassium iodide.

No abstract

Nitrogen is one of the key factors for higher grain production of hybrid maize (Zea mays L.). The use of improved varieties and optimal use of nitrogen (N) fertilizer is important for exploiting yield potential of maize. This work was carried out to evaluate the effects of N rates on grain yield and yield attributing traits of hybrid maize. Two hybrids (RML-86/RML-96 and RML-95/RML-96) and five levels of nitrogen (120, 150, 180, 210 and 240 kg N/ha) were evaluated at research field of National Maize Research Program (NMRP), Rampur, Chitwan, Nepal in spring season of 2017 and 2018. The experiments were conducted in two factorial randomized complete block design (RCBD) with three replications under rainfed condition. The pooled analysis (2017 and 2018) showed that the highest grain yield 10.5 and 10.5 t/ha were given by hybrids RML86/RML96 and RML95/RML96, respectively with the use of 150 kg N/ ha in 2017. The hybrids RML-86/RML-96 and RML-95/RML-96 produced the highest grain yield 7.49 and 7.68 t/ha, respectively with the use of 150 kg N/ha in 2018. The mean grain yield (2017 and 2018) showed that both maize hybrids produced the highest grain yield 10.5 t/ ha in 2017 and 7.58 t/ha year in 2018 with the application of 150 kg N/ha. The application of nitrogen fertilizer on grain yield was found significant (P<0.05) in both years. Therefore, the application of 150 kg N/ha has significantly increased grain yield of maize, Therefore, this dose of nitrogen can be recommended for higher maize production in Chitwan, Nepal in spring season under rainfed condition.

CanSupport has shown that a home-based care model can be successful in India and is desired by patients at the end of life or with chronic illness. Their model of care saves the patients the cost of a hospital visit while still providing evaluation by staff with training in palliative care. In addition, the multidisciplinary nature of the teams allows for symptom management and emotional counseling for both the patients and their families. CanSupport has developed a way to provide reliable, cost-effective palliative care to patients that can serve as a model for building palliative care capacity in low- and middle-income countries.

A rotation experiment was conducted on an alluvial soil at Central Potato Research Station, Jalandhar, India between 1994 and 1999 to develop an integrated nutrient management programme for a potato–sunflower–paddy rice rotation. It tested 10 fertilizer treatments that combined application of 50, 100 and 150% of the recommended rates of inorganic nitrogen (N), phosphorus (P) and potassium (K) with farmyard manure (FYM) and in some cases zinc. Potatoes were the most nutrient-responsive crop followed by paddy rice and sunflower. The responses of potatoes to both P and K were greater in frost and late blight years. In these experiments potatoes required NPK at 150% of the currently recommended rate for maximum tuber production (i.e. they required 270 kg N, 52 kg P and 150 kg K/ha). Application of FYM at 30 t/ha with 270 kg of inorganic N/ha (FYM+N) was less effective than the use of NPK at 150% of the currently recommended rate. Application of K to replace that removed in the harvested crop was more effective in potatoes than giving K at 100 or 150% of the currently recommended rate.Sunflowers grown after potatoes that had received NPK at 150% of the currently recommended rate or with the FYM+N treatment required only half of the currently recommended rate of NPK for maximum yield (i.e. they required 23 kg N, 7 kg P and 13 kg K/ha), whereas paddy rice, grown as a third crop, required the full amount of NPK at the currently recommended rate (i.e. 120 kg N, 26 kg P and 67 kg K/ha). Application of K to adjust for its removal did not benefit sunflowers or paddy rice. Zinc had no direct effect on potatoes or any residual effects on the succeeding sunflower and paddy rice crops. The effects of the FYM+N treatment were cumulative, especially on potatoes which were the most responsive crop, whereas those involving the application of NPK at 150% of the currently recommended rate were not. Also, the NPK fertility of the soil was enhanced and more organic carbon was present following the FYM+N treatment than with NPK fertilizers applied at the currently recommended rate. Application of P resulted in a build-up of P but none of the treatments, even the applications of K to adjust for its removal, maintained the K status of the soil at its initial level.

No abstract

Conventional tillage practices have been regarded as the major reason for the loss of fertile topsoil in the sloping agricultural lands of the middle hills of Nepal. Reports on the effects of no-till and mulch on soil and corn yield in these regions are scarce, although these farming practices have been recommended to reduce soil erosion and increase crop yields. To assess the impacts of tillage (with +T, without −T) and mulch (with +M, without −M) on soil and soil nutrient losses, and corn yield, we conducted an experiment with five treatments: −T+M, −T−M, +T+M, +T−M, and bare fallow (BF), replicated four times each in an unbalanced complete random block design in Salyan district of Nepal. The results showed the presence of corn and no-till significantly lowered the soil losses. Losses of soil organic matter (SOM) and total nitrogen were also significantly reduced by the presence of corn, no-till, and mulch. However, no effects of mulch on soil losses, and no effects of tillage, mulch and corn on soil phosphorus losses were observed. Soil loss was found to be significantly and positively correlated with total seasonal rainfall, monsoon being the most severe season for soil erosion. While no-till and mulch did not affect corn height, cob height, and stover yield, no-till significantly increased the corn yield by 0.52 Mg ha−1 compared to conventional till. We confirm the synergistic interaction of mulch with tillage to reduce the losses of SOM and total nitrogen, and effectiveness of no-till to reduce the soil losses and increase the corn yield in the middle hills of Nepal. As this study is based on the results of two year’s data, long-term studies are required to identify the long-term impacts of no-till and mulch on soil losses and corn yield across the country.

Summary One of the goals of biofortification is to generate iron‐enriched crops to combat growth and developmental defects especially iron (Fe) deficiency anaemia. Fe‐fortification of food is challenging because soluble Fe is unstable and insoluble Fe is nonbioavailable. Genetic engineering is an alternative approach for Fe‐biofortification, but so far strategies to increase Fe content have only encompassed a few genes with limited success. In this study, we demonstrate that the ethyl methanesulfonate (EMS) mutant, iron deficiency tolerant1 (idt1), can accumulate 4–7 times higher amounts of Fe than the wild type in roots, shoots and seeds, and exhibits the metal tolerance and iron accumulation (Metina) phenotype in Arabidopsis. Fe‐regulated protein stability and nuclear localisation of the upstream transcriptional regulator bHLH34 were uncovered. The C to T transition mutation resulting in substitution of alanine to valine at amino acid position 320 of bHLH34 (designated as IDT1A320V) in a conserved motif among mono‐ and dicots was found to be responsible for a dominant phenotype that possesses constitutive activation of the Fe regulatory pathway. Overexpression of IDT1A320V in Arabidopsis and tobacco led to the Metina phenotype; a phenotype that has escalated specificity towards optimising Fe homeostasis and may be useful in Fe‐biofortification. Knowledge of the high tolerance and accumulation of heavy metals of this mutant can aid the development of tools for phytoremediation of contaminants.