Arsenic metabolites; selenium; and AS3MT, MTHFR, AQP4, AQP9, SELENOP, INMT, and MT2A polymorphisms in Croatian-Slovenian population from PHIME-CROME study

Stajnko, Anja; Šlejkovec, Zdenka; Mazej, Darja; France-Štiglic, Alenka; Briški, Alenka Sešek; Prpić, Igor; Špirić, Zdravko; Horvat, Milena; Falnoga, Ingrid

doi:10.1016/j.envres.2018.11.045

Cited by 35 publications

(12 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effects of AS3MT on As metabolism have been widely studied (Kuo et al, 2017;Li et al, 2017). AS3MT polymorphisms (rs11191439, rs1046778, rs3740400, rs11191438, rs3740393, rs9527, rs7085104, rs10748835, and rs3740390) are associated with %uDMA and %uMMA (de la Rosa et al, 2017;De Loma et al, 2018;Gao et al, 2019;García-Alvarado et al, 2018;Huang et al, 2018;Li et al, 2017;Lu et al, 2019;Stajnko et al, 2019;Xu et al, 2016). Epidemiological evidence suggested that SNPs of AS3MT (rs11191439 and rs9527) can significantly alter the level of genetic damage (Das et al, 2016;Sampayo-Reyes et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Most As compounds are excreted in urine (Lu et al, 2019). iAs and its two metabolites are commonly used as biomarkers of exposure (Grau-Perez et al, 2017;Stajnko et al, 2019). The proportion of trivalent As species in the urine reflects the individual's metabolic efficiency (Lu et al, 2019), which is related to the toxic effect of As the individual's metabolic efficiency (Kuo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Several single nucleotide polymorphisms (SNPs) in the AS3MT gene may affect this enzyme activity and gene transcription (Li et al, 2016(Li et al, , 2017Wood et al, 2006). AS3MT polymorphisms, such as rs1046778, rs9527, rs7085104, and rs3740390, are associated with the percentage of DMA in urine (%uDMA) and %uMMA (de la Rosa et al, 2017;Gao et al, 2019;Huang et al, 2018;Lu et al, 2019;Stajnko et al, 2019;Xu et al, 2016), which reflect the capacity to methylate As. Genetic variants of the AS3MT gene are also associated with skin lesions (Das et al, 2016) and the incidence of cancer (de la Rosa et al, 2017;Huang et al, 2018;Lin et al, 2018;Yao et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Polymorphisms of the AS3MT gene are associated with arsenic methylation capacity and damage to the P21 gene in arsenic trioxide plant workers

Tan

Wang

et al. 2021

Toxicol Ind Health

View full text Add to dashboard Cite

Epidemiological evidence suggests that the metabolic profiles of each individual exposed to arsenic (As) are related to the risk of cancer, coronary heart disease, and diabetes. The arsenite methyltransferase ( AS3MT) gene plays a key role in As metabolism. Several single nucleotide polymorphisms in the AS3MT gene may affect both enzyme activity and gene transcription. AS3MT polymorphisms are associated with the proportions of monomethylarsenic acid (MMA) and dimethylarsenic acid (DMA) in urine as well as the incidence of cancer. P21 protein is a cyclin-dependent kinase inhibitor. Mutations of the P21 gene have been found in cancer patients. In our study, we investigate whether polymorphisms of the AS3MT gene alter As methylation capacity and adversely affect the P21 gene in arsenic trioxide plant workers. The DNA damage was examined by the quantitative polymerase chain reaction. Restriction fragment length polymorphism was used to analyze the genotype of the AS3MT gene. The results showed that DNA damage in P21 gene fragments was greater in those individuals exposed to high levels of As. There was a strong positive correlation between the DNA damage to P21 gene fragments and the percentage of MMA in urine. However, DNA damage in P21 gene fragments was negatively associated with the percentage of DMA in urine (%uDMA), primary methylation index (PMI), and secondary methylation index. We found that subjects with the rs7085104 GG or GA allele were associated with higher %uDMA and PMI and less DNA damage. The subjects with the rs11191454 GG+GA or GA allele were also associated with higher %uDMA and PMI and less DNA damage. Our results suggest that rs1191454 and rs7085104 in the AS3MT gene affect the As-induced DNA damage by altering individual metabolic efficiency.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphisms of the AS3MT gene are associated with arsenic methylation capacity and damage to the P21 gene in arsenic trioxide plant workers

Tan

Wang

et al. 2021

Toxicol Ind Health

View full text Add to dashboard Cite

show abstract

“…Table 3 summarises association between rs28366003 SNP and element levels in various healthy population groups or subjects with defined disease. These findings are controversial, as a number of studies found correlations with element concentrations in the human organism ( 84 , 85 , 86 , 89 , 90 , 91 , 104 ) and others did not ( 95 , 99 , 102 , 105 , 106 ).…”

Section: Regulation Of Metallothionein Synthesis and Mt2a Polymorphismsmentioning

confidence: 99%

Metallothionein 2A gene polymorphisms in relation to diseases and trace element levels in humans

Sekovanić

Jurasović

Piasek

2020

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Human metallothioneins are a superfamily of low molecular weight intracellular proteins, whose synthesis can be induced by essential elements (primarily Zn and Cu), toxic elements and chemical agents, and stress-producing conditions. Of the four known isoforms in the human body MT2 is the most common. The expression of metallothioneins is encoded by a multigene family of linked genes and can be influenced by single nucleotide polymorphisms (SNPs) in these genes. To date, 24 SNPs in the MT2A gene have been identified with the incidence of about 1 % in various population groups, and three of them were shown to affect physiological and pathophysiological processes. This review summarises current knowledge about these three SNPs in the MT2A gene and their associations with element concentrations in the body of healthy and diseased persons. The most investigated SNP is rs28366003 (MT2A −5 A/G). Reports associate it with longevity, cancer (breast, prostate, laryngeal, and in paranasal sinuses), and chronic renal disease. The second most investigated SNP, rs10636 (MT2A +838G/C), is associated with breast cancer, cardiovascular disease, and type 2 diabetes. Both are also associated with several metal/metalloid concentrations in the organism. The third SNP, rs1610216 (MT2A −209A/G), has been studied for association with type 2 diabetes, cardiomyopathy, hyperglycaemia, and Zn concentrations. Metallothionein concentrations and MT2A polymorphisms have a potential to be used as biomarkers of metal exposure and clinical markers of a number of chronic diseases. This potential needs to be studied and verified in a large number of well-defined groups of participants (several hundreds and thousands) with a focus on particular physiological or pathological condition and taking into consideration other contributing factors, such as environmental exposure and individual genetic and epigenetic makeup.

show abstract

“…One of the heavy metals that may be present is arsenic (As), which has well-known carcinogenic and toxic effects. [1][2][3][4][5] TiO 2 is a relatively complex matrix with challenging analytics as TiO 2 is usually dissolved in hydrofluoric acid (HF), which can be problematic when glassware equipment is employed (e.g., in inductively coupled plasma mass spectrometry, inductively coupled plasma optical emission spectrometry, electrochemical determination, biosensing, etc.). [6][7][8][9][10][11] The use of hydrogen chloride in the digestion procedure for the determination of As by electrothermal atomic absorption spectrometry (ETAAS) is not recommended because of the formation of volatile metal chlorides.…”

Section: Introductionmentioning

confidence: 99%

Trace Arsenic Determination in a TiO2 Pigment Matrix Using Electrothermal Atomic Absorption Spectrometry

2020

View full text Add to dashboard Cite

This work describes the use of electrothermal atomic absorption spectrometry in combination with a pyrolytic graphite-coated tube with a platform for trace arsenic (As) determination in titanium dioxide (TiO2) pigment. This type of matrix is challenging, as complete digestion in hydrofluoric acid-containing solution is needed. First, closed-vessel microwave digestion was performed for the full-sample decomposition. Next, a temperature program was optimized for drying, pyrolysis, and atomization temperatures. Furthermore, the use of a chemical modifier mixture was proposed that reduced the background contribution and prevented significant analyte loss and therefore improved the analytical procedure. The optimized method was validated for the detection (LOD) and quantification (LOQ) limits, the linear concentration range, accuracy, and precision. Special attention was devoted to the matrix-matching solutions in the calibration procedure. Linearity was confirmed in the 5.0 to 100.0 µg/L concentration range ( R2 = 0.999). The average recovery for 16 different real TiO2 pigment samples was 92.0%, and the relative standard deviation value for six replicate measurements was ≤10.4%. Moreover, the LOD and LOQ in terms of the TiO2 pigment mass was determined to be 0.2 µg/(g TiO2) and 0.7 µg/(g TiO2), respectively. The latter complies with Commission Directive 2008/128/EC, which does not allow more than 3 µg As/(g product) as the specific criteria of purity. Finally, based on scanning electron microscopy analysis of unused and several times used pyrolytic graphite-coated tubes, usage of the tube 250 times before replacement is recommended.

show abstract

Arsenic metabolites; selenium; and AS3MT, MTHFR, AQP4, AQP9, SELENOP, INMT, and MT2A polymorphisms in Croatian-Slovenian population from PHIME-CROME study

Cited by 35 publications

References 77 publications

Polymorphisms of the AS3MT gene are associated with arsenic methylation capacity and damage to the P21 gene in arsenic trioxide plant workers

Polymorphisms of the AS3MT gene are associated with arsenic methylation capacity and damage to the P21 gene in arsenic trioxide plant workers

Metallothionein 2A gene polymorphisms in relation to diseases and trace element levels in humans

Trace Arsenic Determination in a TiO2 Pigment Matrix Using Electrothermal Atomic Absorption Spectrometry

Contact Info

Product

Resources

About